Effects of ursodeoxycholic acid, glutamine and polyclonal immunoglobulins on bacterial translocation in common bile duct ligated rats

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dc.contributor.authorAldemir, M
dc.contributor.authorGeyik, MF
dc.contributor.authorKökoglu, ÖF
dc.contributor.authorBüyükbayram, H
dc.contributor.authorHosoglu, S
dc.contributor.authorYagmur, Y
dc.date.accessioned2024-04-24T16:24:06Z
dc.date.available2024-04-24T16:24:06Z
dc.date.issued2003
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: The present study was conducted to investigate the effects of ursodeoxycholic acid (UDCA), glutamine and i.v. polyclonal immunoglobulins ( IVIG) on the bacterial translocation (BT) and intestinal integrity of obstructive jaundice (OJ) in an animal model. Methods: Fifty rats were randomized into five groups containing 10 rats each. All procedures were performed aseptically under general anaesthesia using intramuscular ketamine ( 25 mg/kg). The abdomen was opened and the common bile duct was identified, mobilized, doubly ligated using 5-0 silk and divided. In group 1 ( the 'sham' group), the rats had a similar incision followed by mobilization of the common bile duct (CBD), without ligation or division. In group 2 rats, only common bile duct ligation (CBDL) was performed. In group 3, CBDL was performed and UDCA was administered by orogastric intubation once daily. In group 4 rats, CBDL was performed and glutamine was given by orogastric intubation once daily. Therapeutic substances were started orally on the day CBDL was fulfilled and were continued for 7 days. In group 5, IVIG was administrated via a femoral vein catheter just before CBDL. The animals were killed at the end of the 7th day, and serum levels of total bilirubin ( TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (GGT) were measured. Mesenteric lymph nodes (MLN), liver, spleen and blood were cultured. The terminal ileum specimens were examined histopathologically. Results: Bacterial translocation significantly increased in the MLN and spleen of rats in group 2 as compared to groups 3, 4 and 5 (P < 0.05, P = 0.001, P = 0.001, respectively). The BT of the liver in group 2 was significantly higher than that of group 5 (P < 0.05). In the blood, the BT was significantly higher in group 2 than groups 3, 4 and 5 ( P < 0.05). The bacterial counts, colony- forming units per gram tissue (cfu/g), were found significantly higher in MLN, liver and spleen of rats in group 2 than those of groups 3, 4 and 5 ( P = 0.000). The average villus height in the group 4 was significantly higher than that of groups 2, 3 and 5 ( P = 0.000). Conclusion: The present experimental study has demonstrated that the administration of glutamine, UDCA and IVIG reduce the incidence of BT and additionally glutamine preserves intestinal mucosal integrity.en_US
dc.identifier.doi10.1046/j.1445-2197.2003.02749.x
dc.identifier.endpage726en_US
dc.identifier.issn1445-1433
dc.identifier.issue9en_US
dc.identifier.pmid12956789
dc.identifier.scopus2-s2.0-0142075899
dc.identifier.scopusqualityQ2
dc.identifier.startpage722en_US
dc.identifier.urihttps://doi.org/10.1046/j.1445-2197.2003.02749.x
dc.identifier.urihttps://hdl.handle.net/11468/16491
dc.identifier.volume73en_US
dc.identifier.wosWOS:000185196800013
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBlackwell Publishing Asiaen_US
dc.relation.ispartofAnz Journal of Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacterial Translocationen_US
dc.subjectGlutamineen_US
dc.subjectIvigen_US
dc.subjectObstructive Jaundiceen_US
dc.subjectUrsodeoxycholic Aciden_US
dc.titleEffects of ursodeoxycholic acid, glutamine and polyclonal immunoglobulins on bacterial translocation in common bile duct ligated ratsen_US
dc.titleEffects of ursodeoxycholic acid, glutamine and polyclonal immunoglobulins on bacterial translocation in common bile duct ligated rats
dc.typeArticleen_US

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