Increased ROS alters E-/N-cadherin levels and promotes migration in prostate cancer cells

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dc.authorid0000-0002-4337-9327en_US
dc.authorid0000-0003-0172-6957en_US
dc.contributor.authorVarisli, Lokman
dc.contributor.authorTolan, Veysel
dc.date.accessioned2022-09-02T12:12:39Z
dc.date.available2022-09-02T12:12:39Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractBackground: Increased ROS has been reported to cause a change in E- and N-cadherin levels, and consequently promotes migrative behaviors in pancreas and breast cancer cells. In this study, the effect of a sublethal dose of H2O2 on E- and N-cadherin levels, and on migrative behaviors of PCa cells was investigated. Methods: To determine a sublethal concentration of H2O2 on cell proliferation and ROS production were examined using WST-1 and DCFH-DA assays, respectively. E- and N-cadherin protein and mRNA levels were investigated by western blotting and real-time PCR, respectively. The migrative potentials of the cells were examined by Cytoselect 96-well cell migration assay. Results: Treatment of the PCa cells with a sublethal dose of H2O2 results in a decrease in E-cadherin and an increase in N-cadherin levels, at both mRNA and protein levels. However, inhibition of ERK using PD98059 abolishes the effects of H2O2. In addition, the cells that were treated with H2O2 have gained further migrative abilities compared to control cells, and this ability was repressed when PD98059 was used together with H2O2. Conclusion: Increased ROS alters E- and N-cadherin levels in an ERK-dependent manner and thereby promotes the migrative abilities of PCa cells.en_US
dc.identifier.citationVarışli, L. ve Tolan, V. (2022). Increased ROS alters E-/N-cadherin levels and promotes migration in prostate cancer cells. Bratislava Medical Journal- Bratislavske Lekarske Listy, 1-6.en_US
dc.identifier.doi10.4149/BLL_2022_121
dc.identifier.endpage6en_US
dc.identifier.pmid35913012
dc.identifier.scopus2-s2.0-85138144855
dc.identifier.scopusqualityN/A
dc.identifier.startpage1en_US
dc.identifier.urihttp://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7761&category_id=164&option=com_virtuemart&vmcchk=1&Itemid=1
dc.identifier.urihttps://hdl.handle.net/11468/10320
dc.identifier.wosWOS:000858637000011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorVarışli, Lokman
dc.institutionauthorTolan, Veysel
dc.language.isoenen_US
dc.publisherAepressen_US
dc.relation.ispartofBratislava Medical Journal- Bratislavske Lekarske Listy
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US
dc.relation.tubitak114S108
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectE-cadherinen_US
dc.subjectN-cadherin cell migrationen_US
dc.subjectROSen_US
dc.subjectProstate canceren_US
dc.titleIncreased ROS alters E-/N-cadherin levels and promotes migration in prostate cancer cellsen_US
dc.titleIncreased ROS alters E-/N-cadherin levels and promotes migration in prostate cancer cells
dc.typeArticleen_US

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