Heterofunctional magnetic metal-chelate-epoxy supports for the purification and covalent immobilization of benzoylformate decarboxylase from pseudomonas putida and its carboligation reactivit

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dc.contributor.authorTural, Servet
dc.contributor.authorTural, Bilsen
dc.contributor.authorDemir, Ayhan Sıtkı
dc.contributor.orcid0000-0003-2479-9438
dc.contributor.orcid0000-0001-7555-2481
dc.contributor.orcidTural, S., Tural, B. ve Demir, A. S. (2015). Heterofunctional magnetic metal-chelate-epoxy supports for the purification and covalent immobilization of benzoylformate decarboxylase from pseudomonas putida and its carboligation reactivit. Chirality, 27(9), 635-642.
dc.date.accessioned2024-04-24T15:59:22Z
dc.date.available2024-04-24T15:59:22Z
dc.date.issued2015
dc.departmentDicle Üniversitesi, Ziya Gökalp Eğitim Fakültesi, Matematik ve Fen Bilimleri Eğitimi Bölümüen_US
dc.description.abstractIn this study, the combined use of the selectivity of metal chelate affinity chromatography with the capacity of epoxy supports to immobilize poly-His-tagged recombinant benzoylformate decarboxylase from Pseudomonas putida (BFD, E.C. 4.1.1.7) via covalent attachment is shown. This was achieved by designing tailor-made magnetic chelate-epoxy supports. In order to selectively adsorb and then covalently immobilize the poly-His-tagged BFD, the epoxy groups (300 mu mol epoxy groups/g support) and a very small density of Co2+-chelate groups (38 mu mol Co2+/g support) was introduced onto magnetic supports. That is, it was possible to accomplish, in a simple manner, the purification and covalent immobilization of a histidine-tagged recombinant BFD. The magnetically responsive biocatalyst was tested to catalyze the carboligation reactions. The benzoin condensation reactions were performed with this simple and convenient heterogeneous biocatalyst and were comparable to that of a free-enzyme-catalyzed reaction. The enantiomeric excess (ee) of (R)-benzoin was obtained at 99 +/- 2% for the free enzyme and 96 +/- 3% for the immobilized enzyme. To test the stability of the covalently immobilized enzyme, the immobilized enzyme was reused in five reaction cycles for the formation of chiral 2-hydroxypropiophenone (2-HPP) from benzaldehyde and acetaldehyde, and it retained 96% of its original activity after five reaction cycles. Chirality 27:635-642, 2015. (c) 2015 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipMiddle East Technical University, Dicle University Research Fund [13-ZEF-28.13-ZEF-85.109T497]; Scientific and Technological Research Council of Turkeyen_US
dc.description.sponsorshipContract grant sponsor: financial support from Middle East Technical University, Dicle University Research Fund; Contract grant numbers: 13-ZEF-28.13-ZEF-85.109T497.; Contract grant sponsor: Scientific and Technological Research Council of Turkey.en_US
dc.identifier.doi10.1002/chir.22477
dc.identifier.endpage642en_US
dc.identifier.issn0899-0042
dc.identifier.issn1520-636X
dc.identifier.issue9en_US
dc.identifier.pmid26147067
dc.identifier.scopus2-s2.0-84939466593
dc.identifier.scopusqualityQ2
dc.identifier.startpage635en_US
dc.identifier.urihttps://doi.org/10.1002/chir.22477
dc.identifier.urihttps://hdl.handle.net/11468/14015
dc.identifier.volume27en_US
dc.identifier.wosWOS:000360080900011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTural, Servet
dc.institutionauthorTural, Bilsen
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofChirality
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmobilized benzoylformate decarboxylaseen_US
dc.subjectMetal-chelate-epoxy magnetic supporten_US
dc.subjectMetal-affinity magnetic nanoparticlesen_US
dc.subjectSelective immobilizationen_US
dc.subjectCovalent attachmenten_US
dc.subjectOne step purificationen_US
dc.titleHeterofunctional magnetic metal-chelate-epoxy supports for the purification and covalent immobilization of benzoylformate decarboxylase from pseudomonas putida and its carboligation reactiviten_US
dc.titleHeterofunctional magnetic metal-chelate-epoxy supports for the purification and covalent immobilization of benzoylformate decarboxylase from pseudomonas putida and its carboligation reactivit
dc.typeArticleen_US

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