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    Antitumor activity of irinotecan with ellagic acid in C6 glioma cells
    (Assoc Medica Brasileira, 2022) Cetin, Abdurrahman; Biltekin, Burcu; Ozevren, Huseyin
    OBJECTIVE: Irinotecan-based combination chemotherapies in malignant gliomas need to be examined. The aim of this study was to investigate the synergetic effect of ellagic acid, a natural polyphenolic antioxidant compound, with irinotecan, an inhibitor of topoisomerase I enzyme, on the growth, cadherin switch, and angiogenic processes of a glioma cell line. METHODS: A combination of 100 mu M ellagic acid and 100 mu M irinotecan was applied to rat C6 glioma cells for 24th, 48th, and 72nd h. The cell proliferation was evaluated by 5-bromo-2'-deoxyuridine immunocytochemistry. The expression levels of vascular endothelial growth factor, E-cadherin, and N-cadherin were measured using real-time polymerase chain reaction and their immunoreactivities using immunocytochemistry. RESULTS: The treatment of irinotecan with combining ellagic acid enhanced antitumor activity and the synergistic effect of these reduced the cell proliferation of C6 glioma by inhibiting the cadherin switch and promoting the antiangiogenic processes. CONCLUSIONS: Further research is required to prove a negative relationship between C6 glial cell proliferation and irinotecan with ellagic acid application. Our preliminary data suggest that even with the extremely short-term application, irinotecan with ellagic acid may affect glioma cells at the level of gene and protein expression.
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    Comparison of histopathologic findings of initial and recurrent lumbar disc herniation
    (Taylor & Francis Ltd, 2024) Ozevren, Huseyin; Cetin, Abdurrahman; Baloglu, Murat; Deveci, Engin
    ObjectiveRecurrent lumbar disc hernia (RLDH) is a common and challenging complication after an initial discectomy. This study aimed to investigate the relationship between the histopathologic outcomes of the initial and recurrent disc tissues.MethodsThis study investigated 70 patients who underwent a microdiscectomy and subsequently developed same-level same-side lumbar disc herniation (LDH) recurrence. The clinic, western blot, and immunohistochemical evaluations of patients with initial LDH and RLDH were conducted and statistically analyzed.ResultsThe effect of inflammation and apoptosis in the degenerative changes of intervertebral disc hernia and increased histopathologic findings in RLDH was demonstrated. The degeneration of the hernia disc tissue is a major pathological process, which is characterized by cellular apoptosis, inflammation, and reduced synthesis of extracellular matrix. Currently, there is no clinical therapy targeting the reversal of disc degeneration.ConclusionsThis, therefore, stay away from factors that increase inflammation in the intervention of intervertebral disc hernia, applying to reduce inflammation the medicines, could allow reducing disc collagen degeneration, and more successful outcomes. These findings might shed some new lights on the mechanism of disc degeneration and provide new strategies for the treatments of initial and recurrent LDH.
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    Determining the Optimal Length and Safety of Pedicle Screws in the T12 Vertebra: A Morphometric Study
    (Springernature, 2018) Korkmaz, Mehmet F.; Erdem, Mehmet N.; Ozevren, Huseyin; Sevimli, Resit
    Introduction: Despite the developments in implant technology and imaging methods and the advances in surgical techniques, there are still potential problems and complications of transpedicular screw application. This is a morphometric study to examine the proximity of the T12 vertebra to the thoracic aorta. Our aim was to define the appropriate length of the pedicle screw to be used in the 12 th thoracic vertebra, using computed tomography (CT) data. Methods: Randomly selected cases from the same ethnic group in a specific age group were examined in terms of the length from the anterior vertebral body and the screw entry point of the T12 vertebra to the thoracic aorta. In light of these data, a statistical analysis was made for the selection of the most appropriate screw length. Results: A statistically significant difference was detected in the distance from the T12 left screw entry point to the aorta between males and females (p=0.001). No statistically significant correlation was found between age and the distance between the left screw entry point and the aorta (p=0.105). Also, no statistically significant difference was detected between the T12 vertebral body-aorta distance in males and in females (p=0.212). The relationship between the shortest aorta-vertebral body distance and age was not statistically significant (p=0.7). Similarly, there was no statistically significant difference between the left screw entry pointaorta distance and the aorta-vertebral body shortest distance (p=0.731). Conclusions: Significant differences were observed between males and females in terms of the distance between the T12 vertebra left screw entry point and the thoracic aorta (p=0.001). Thus, we can assert the need for the preoperative evaluation of patients with computed tomography in selecting the appropriate screw length and avoiding complications.
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    Effects of Nicotine on Rat Alveolar Bone
    (Sci Printers & Publ Inc, 2016) Eratilla, Veysel; Uysal, Ibrahim; Ozevren, Huseyin; Uysal, Rojdan Ferman Gunes; Aktas, Elif Eratilla; Karabulut, Ozlen; Deveci, Engin
    OBJECTIVE: To investigate the effects of nicotine on periodontal membranes and alveolar bone using histopathological and immunohistochemical methods. STUDY DESIGN: Rats were divided into 2 groups: those systemically treated with nicotine sulfate (2 mg/kg subcutaneously, daily) for 28 days, and untreated controls. We analyzed the distribution of osteonectin and osteopontin immunohistochemically, with antibodies, in the maxillary region. RESULTS: In the treatment group, resorption of alveolar bone and vascular dilation, bleeding, periodontal membrane degeneration, hyalinization, and necrosis were seen in some areas. Negative osteoblastic activity was observed in immunohistochemical analyses of collagen fibers in the periodontal membrane. CONCLUSION: In short- and long-term nicotine consumption, soft tissue and alveolar bone are negatively affected during cell proliferation. This phenomenon may lead to a delay in treatment of implantation.
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    Effects of Xylene on Respiratory Mucosa in Rats
    (Soc Chilena Anatomia, 2016) Eda, Arslan; Baver, Samanci; Seyla, Samanci Bolukbasi; Ozevren, Huseyin; Deveci, E.
    In this study we examined the effects histopathologic and immunohistochemical of xylene inhalation in rats by using light microscopy. Adult wistar albino rats were used in this study. Eight rats were in control group and 8 rats were in the experimental group. The experimental group was exposed to 300 ppm formaldehyde 3-5 min/day, 5 days/week for 8 weeks. The lining epithelium of respiratory mucosa showed a loss of ciliated cells with metaplasia of goblet cells, hyperplasia of squamous cells and edema, inflamation in sub epithelial area). In the group treated xylene. Disruption of cell-cell contact was observed. Weak expression of E-cadherin was observed between cells. The vascular endothelium of capillaries and venoles showed intense immunostaining for VEGF.
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    Evaluation of the association between biochemical and immunohistochemical score of caspase-9 and TNF?, and the grading of lumbar disc herniation
    (Taylor & Francis Ltd, 2021) Ozevren, Huseyin; Cetin, Abdurrahman; Baloglu, Murat; Deveci, Engin
    Objective The aim of our study is to investigate the influence of caspase-9 and tumour necrosis factor alpha (TNF alpha) in the grade of lumbar disc herniation. We determined the strength of different predictors such as age, gender, disc grading, caspase-9 and TNF alpha. Methods We retrospectively reviewed 84 patients who had discectomies. Histological and biochemical evaluations of disc specimens were performed. All patients were scanned by the magnetic resonance imaging (MRI) scanner before the operation. Masson's trichrome stain, biochemical analysis and immunohistochemical staining were performed to measure the expression levels of caspase-9 and TNF alpha. The results were evaluated statistically. Results This study included 84 patients (mean age: 41.59 +/- 12.21 years; range: 19-76): 60 men (age 40.47 +/- 12.63 years) and 24 women (44.42 +/- 10.81 years). No statistically significant age difference was found between the genders (p = 0.182). MRI scans showed 16 patients had protrusion, 44 had extrusion and 24 had sequestration of discs. There was a statistically significant negative correlation between the grading of lumbar disc herniations and age (p < 0.001,r = -0.509). Histological and biochemical analyses of disc materials were done. Inflammation, collagen fibre deterioration, apoptotic process, TNF alpha and caspase-9 were seen to increase with increasing disc grading (p < 0.01). Conclusions Biochemical and immunohistochemical score of caspase-9 and TNF alpha indicate the grading of lumbar disc herniation. As the grading of disc herniation increases, inflammation of cells and collagen fibre disruption increase and accelerate the apoptotic process. Apoptosis in disc nucleus pulposus cells may reduce disc herniation.
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    Immunohistochemical and Histopathological Changes in the Skin of Rats after Administration of Lead Acetate
    (Soc Chilena Anatomia, 2016) Sula, Bilal; Deveci, Engin; Ozevren, Huseyin; Ekinci, Cenap; Elbey, Bilal
    Lead acetate is a chemical compound. Sources of human exposure to this metal include many foods, drinking water and dust. The aim of this study was to determine the immunohistochemical and histopathological changes on the face skin after lead acetate application. Wistar Albino rats (180-200 g body weight) were divided into a controlled and lead acetate-exposed group. Rats received lead acetate at 500 ppm in their drinking water for 60 days. Both groups were fed with the same standard food, but lead acetate was added to the drinking water. During the experimental period, blood samples were drawn from the abdominal aorta of the anesthetised animals. At the end of exposure, body weight and blood lead levels were measured. Sections of rat facial skin were examined histopathological and immunohistochemical. In the group treated with lead acetate, minimal to slight multifocal hydropic degeneration of basal cell layer, depending on the thinning of the epidermis, the cellular degeneration in the dermis and a increase in the number of necrotic cells was observed in sebaceous glands of the hair follicle hemorrhage. The immunohistochemical results of the present work demonstrated an increase in Proliferating cell nuclear antigen (PCNA) immunoreactivity in skin specimens from lead acetate treated animals. Vimentin immunoreactivity was very dense in hair follicle of the subepidermal region. It was also strongly stained around the myoepithelial cells surrounding sebaceous and stromal cells.
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    Immunohistochemical and Ultrastructural Examination of Changes in the Retina in Diabetic Rats
    (Sci Printers & Publ Inc, 2016) Turgut, Fethiye Gulden; Ozevren, Huseyin; Irtegun, Sevgi; Deveci, Engin
    OBJECTIVE: To study diabetic retinopathy by the immunohistochemical and ultrastructural examination of changes in the retina in diabetic rats. STUDY DESIGN: We examined the blood-retinal barrier and cell-to-cell connections in diabetic rats by immunohistochemistry and ultrastructural level. Twenty adult male Wistar rats were used in this study. The animals were divided into 2 groups. The control group was fed standard rat chow and drinking water for 8 weeks. Single-dose streptozotocin (55 mg/kg) was dissolved in sodium citrate buffer and administered via intraperitoneal injection to induce diabetes in the diabetes group. The diabetes and control groups were compared for glucose values. RESULTS: The blood glucose concentration in diabetic rats was significantly increased (p< 0.05). Dilation of blood vessels, hemorrhage, and edema were observed in the nerve fibers and ganglion layer. An increase in vascular endothelial growth factor expression was confirmed by Western blot method. E-cadherin-positive cells in the immune junctions between cells in the diabetic retina were observed, weakly staining. CONCLUSION: Ultrastructural analysis of diabetic retinopathy showed that dysfunctionality of photoreceptor cells and vision loss are thought to occur due to mitochondrial degeneration. (Anal Quant Cytopathol Histpathol 2016; 38: 357-363)
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    Neuroprotective Effects of Lamotrigine on Brain Injury in Rats
    (Sci Printers & Publ Inc, 2017) Ozevren, Huseyin; Deveci, Engin
    OBJECTIVE: To investigate the protective role of lamotrigine (LTG), which has a neuroprotective effect on secondary damage induced in the head trauma model. STUDY DESIGN: Sixty-four rats were divided into 4 groups: group 1 (vehicle-treated control), group 2 (LTG 10 mg/kg/day), group 3 (vehicle-treated trauma), and group 4 (trauma+ LTG 10 mg/kg/day). Distilled water was used as vehicle. All rats were decapitated 48 hours after the induction of trauma, and the protective effects of LTG were evaluated by histological, behavioral, immunohistochemical, and biochemical analyses. RESULTS: Administration of LTG at a dose of 10 mg/kg/day provided significant improvement in all of the histological, biochemical, and behavioral parameters after the induction of traumatic brain injury. Trauma caused a significant alteration in the neurological examination scores at 48 hours (p< 0.001). Although the scores were still higher in the LTG-treated group, it was significantly improved as compared with the trauma group (p< 0.01). CONCLUSION: In this study, histopathological scoring was performed in the control, trauma, and LTG groups. p< 0.0001 values were found to be significant in comparison between groups. Vascular endothelial growth factor expressions were compared between the groups. p< 0.0001 values were found to be significant. Glial fibrillary acidic protein expres-sions were compared between the groups. p=0.0004 values were found. Although further studies are necessary to evaluate the time-and-dose-dependent neuroprotective effects of LTG, it may be a beneficial therapeutic agent for prevention of secondary neuronal damage following diffuse traumatic brain injury.
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    Neuroprotective Effects of Potentilla Fulgens on Traumatic Brain Injury in Rats
    (Sci Printers & Publ Inc, 2017) Ozevren, Huseyin; Irtegun, Sevgi; Deveci, Engin; Ozgur, Mustafa Esref; Asir, Firat; Tekin, Mehmet Ali; Deveci, Senay
    OBJECTIVE: To investigate if Potentilla fulgens (P. fulgens) has any neuroprotective effects on traumatic brain injury in rats. STUDY DESIGN: Sprague-Dawley rats were subjected to traumatic brain injury with a weight-drop device using 300 g(-1) m weight-height impact. Sixty-four rats were divided into 4 groups: group 1 (vehicle-treated control), group 2 (P. fulgens 400 mg/kg/day, intraperitoneally [i.p.]), group 3 (vehicle-treated trauma), and group 4 (trauma+P. fulgens 400 mg/kg/day, i.p.). Distilled water was used as vehicle. All rats were decapitated 5 days after the induction of trauma, and the protective effects of P. fulgens were evaluated by histological, immunohistochemical, and biochemical analyses. RESULTS: Administration of P. fulgens at a dose of 400 mg/kg/day provided significant improvement in all of the histological, biochemical, and immunohistochemical analyses after the induction of traumatic brain injury. CONCLUSION: Although further studies are necessary to evaluate the time-and dose-dependent neuroprotective effects of P. fulgens, it may be a beneficial therapeutic agent for prevention of secondary neuronal damage following diffuse traumatic brain injury.
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    Protective Effect of Potentilla fulgens on Changes in Cerebellum Following Traumatic Head Injury
    (Sci Printers & Publ Inc, 2018) Ozevren, Huseyin; Irtegun, Sevgi; Deveci, Engin
    OBJECTIVE: To examine protective effects of Potentilla fulgens (P. fulgens) extract on cerebellar tissue damage of head-traumatized rats with respect to biochemical, histopathological, immunohistochemical, and ultrastructural analysis. STUDY DESIGN: Sprague-Dawley rats were subjected to traumatic head injury with a weight-drop device using 300 g-1 m weight-height impact. In our study the occipital lobe region was subjected to moderate trauma. Sixty-four rats were divided into 4 groups: group 1 (control group), group 2 (P. fulgens group [400 mg/kg/day i.p.]), group 3 (trauma group), and group 4 (trauma +P. fulgens [400 mg/kg/day i.p.]). Distilled water was used as vehicle. All rats were decapitated 5 days after the induction of trauma, and the protective effects of P. fulgens were evaluated by histological, immunohistochemical, and biochemical analyses. RESULTS: After trauma, degenerative changes and congestion were observed in Purkinje and glial cells. Elevated expression of E-cadherin in synaptic junctions, increased tumor necrosis factor alpha expression in Purkinje and glial cells, and elevated endothelin-1 expression in vessel endothelium were observed. Ultra-structural analysis showed loss of mitochondrial crista, and dilation of endoplasmic reticulum of Purkinje cells was observed. CONCLUSION: Although further studies are necessary to evaluate the time- and dose-dependent neuroprotective effects of P. fulgens, it may be a beneficial therapeutic agent for prevention of cerebellar damage following diffuse traumatic head injury.
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    Protective Effects of Ganoderma lucidum on the Changes Made in the Retinal Damage Induced by Traumatic Head Injury
    (Soc Chilena Anatomia, 2017) Ozevren, Huseyin; Deveci, Engin
    Head trauma affects the optic nerve visual function and visual acuity. As a result of head trauma occurring in the retina of the various biochemical, histological and immunohistochemical effects were investigated. The protective effect of Ganoderma lucidum was evaluated on the damage to the retina of the rats. Sprague-Dawley rats were subjected to traumatic brain injury with a weight-drop device using 300 g-1 m weight-height impact. Thirty rats were divided into three groups as group 1 control, 2 group trauma, 3 group trauma+Gonoderma lucidum (20 mL/kg per day via gastric gavage) Ganoderma lucidum was administered for 7 days after trauma. All rats were decapitated 5 days after the induction of trauma, and the protective effects of Ganoderma lucidum in retina were evaluated by histological, immunohistochemical and biochemical analyses. The antioxidant effect of Ganoderma lucidum on the cellular degeneration extracellular matrix and retinal barrier in retina after head trauma was investigated.