Yazar "Kolak, Ufuk" seçeneğine göre listele

Listeleniyor 1 - 20 / 22
  • [ X ]
    Öğe
    Anticholinesterase and Antioxidant Activities of Natural Abietane Diterpenoids with Molecular Docking Studies
    (Bentham Science Publ Ltd, 2020) Topcu, Gulacti; Akdemir, Atilla; Kolak, Ufuk; Ozturk, Mehmet; Boga, Mehmet; Bahadori, Fatemeh; Cakmar, Seda Damla Hatipoglu
    Background: Alzheimer's Disease (AD) is one of the most prevalent causes of dementia in the world, and no drugs available that can provide a complete cure. Cholinergic neurons of the cerebral cortex of AD patients are lost due to increased activity of cholinesterase enzymes. Objective: Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE) are the two major classes of cholinesterases in the mammalian brain. The involvement of oxidative stress in the progression of AD is known. Thus, the objective of this study is to determine strong ChE inhibitors with anti-oxidant activity. Methods: In this study, 41 abietane diterpenoids have been assayed for antioxidant and anticholinesterase (both for AChE and BuChE) properties in vitro, which were previously isolated from Salvia species, and structurally determined by spectroscopic methods, particularly intensive 1D- and 2D-NMR and mass experiments. Molecular modeling studies were performed to rationalize the in vitro ChE inhibitory activity of several abietane diterpenoids compared with galantamine. Results: Thirteen out of the tested 41 abietane diterpenoids exhibited at least 50% inhibition on either AChE or BuChE. The strongest inhibitory activity was obtained for Bractealine against BuChE (3.43 mu M) and AChE (33.21 mu M) while the most selective ligand was found to be Hypargenin E against BuChE enzyme (6.93 mu M). A full correlation was not found between anticholinesterase and antioxidant activities. The results obtained from molecular modelling studies of Hypargenin E and Bractealine on AChE and BuChE were found to be in accordance with the in vitro anti-cholinesterase activity tests. Conclusion: Abietane diterpenoids are promising molecules for the treatment of mild-moderate AD.
  • [ X ]
    Öğe
    Antioxidant, anticholinesterase, and antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm.
    (Tubitak Scientific & Technological Research Council Turkey, 2014) Ertas, Abdulselam; Boga, Mehmet; Hasimi, Nesrin; Yesil, Yeter; Goren, Ahmet Ceyhan; Topcu, Gulacti; Kolak, Ufuk
    This study was the first fatty acid analysis and biological activity report on an endemic Achillea species, Achillea cappadocica Hausskn. et Bornm. The major compounds in the fatty acid were identified as oleic (34.7%), palmitic (23.1%), and linoleic acids (20.6%). The methanol and water extracts indicated higher ABTS cation radical scavenging activity than that of alpha-tocopherol and BHT at 100 mu g/mL. The acetone extract showed 70.62% inhibitory activity against butyrylcholinesterase at 200 mu g/mL. The acetone and methanol extracts exhibited moderate antimicrobial activity.
  • [ X ]
    Öğe
    Bazı Salvia (Ada Çayı) Türlerinin HPLC-IT-TOF-MS ile Taranması, Sekonder Metabolitlerinin Saflaştırılması ve Antikanser Özelliklerinin Belirlenmesi
    (2018) Yılmaz, Mustafa Abdullah; Yener, İsmail; Ertaş, Abdulselam; Temel, Hamdi; Topçu, Gülaçtı; Kandemir, Sevgi İrtegün; Kolak, Ufuk
    Arazi çalışmaları sonucu toplanan 6 Salvia türünün ön biyolojik aktivite deneylerinde kullanılmak üzere kloroform ve etanol ekstreleri hazırlandı. Bu 12 ekstreden aktif çıkan etanol ekstrelerinden saf madde izolasyonuna gidilmiştir. Özellikle izolasyon çalışmaları için klorofil ve türevleri çok ciddi problemlere yol açmaktadır. En azından kök ksımlarının ekstreleri hazırlanarak izolasyon çalışmalarında klorofil ve türevlerinin neden olacağı problemlerin önüne geçilmesi düşünülmüştür. Toplanan türlerin LC-MS/MS ile fenolik içeriklerinin tespiti için çeşitli kısımlarının etanol ekstreleri hazırlanmıştır. Ayrıca bu türlerin yağ asidi bileşimini GC-MS ile belirlemek için petrol eteri ekstreleri hazırlandı. Bunlara ilaveten 3 yıl boyunca toplanan örneklerin uçucu yağı elde edilmiştir. 3 yıl boyunca toplanan örneklerin GC-MS ile uçucu yağ ve aroma içerikleri belirlenmiştir. Çalışılan türlerin uçucu yağ verimlerinin, aroma ve uçucu yağ içeriklerinin yıllara bağlı olarak çok değişmediği tespit edilmiştir. Ayrıca aroma ve uçucu yağ içeriklerinin daha çok monoterpenler bakımından zengin olduğu saptanmıştır. Yine GC-MS ile belirlenen yağ asidi içeriğinin de yıllara bağlı olarak çok değişmediği belirlenmiştir. Ayrıca Salvia türlerine özgü 19 bileşik ile yeni bir metot geliştirilerek valide edilmiştir. Üç yıl toplanan örneklerin bu bileşikler bakımından içerik tayinleri yine LC-MS/MS ile tespit edilmiştir. Çalışılan Salvia türlerinin çeşitli kısımlarının farklı ekstrelerinin toplam fenolik ve toplam flavonoit miktarları belirlenmiştir. Hazırlanan tüm ekstrelerin 4 farklı yöntemle antioksidan aktiviteleri belirlenmiştir. Çalışılan 6 Salvia türünün aktivite sonuçlarına baktığımızda genel olarak petrol eteri ekstrelerinin daha çok sadece ?-Karoten-linoleik asit yönteminde etanol ekstrelerinin ise kullanılan 4 yöntemde de aktif olduğu tespit edilmiştir. Çalıştığımız 6 Salvia türünün kloroform ve etanol ekstrelerinin sitotoksik aktivitesi MTT metoduna göre 3 hücre serisine karşı belirlenmiştir. Çalışılan 12 ekstrenin sonuçları genel olarak değerlendirildiğinde ancak yüksek konsantrasyonlarda HT29 ve MCF7 hücre serilerine karşı sitotoksik etki gösterdiği belirlenmiştir. Ekstrelerin sitotoksik etkilerinin düşük antioksidan etkilerinin yüksek çıkmasından dolayı izolasyon çalışmalarının antiokisdan kontrolü ile yapılmasına karar verilmiştir. Etanol ekstrelerinin kloroform ekstrelerine göre daha yüksek antioksidan aktivite göstermelerinden dolayı saf madde izolasyonuna bu ekstrelerden gidilmiştir. 5 Türden (Toprak üstü ve kök olmak üzere) hazırlanan 10 ekstre kolon kromatografisi ile fraksiyonlara ayrılmıştır. Bu fraksiyonlardan antioksidan aktiviteleri yüksek çıkanlardan izolasyon çalışmaları yapılmıştır. İzolasyon çalışmalarında 4?ü yeni olmak üzere toplam 52 bileşik elde edilmiştir. İzole edilen maddelerin antioksidan ve sitotoksik aktiviteleri tespit edilmiştir. Genel olarak preperatif HPLC ile elde edilen maddelerin çok yüksek bir antiokidan kapasitye sahip olduğu belirlenmiştir. Özellikle yüksek miktarda elde edilen 6,7-dehidroroyleanon bileşiğinin ve türevi olan asetil royleanon bileşiklerinin elde edilen terpenler içinde en yüksek antioksidan aktiviteye sahip olduğu tespit edilmiştir. Yapısı bilinen maddelerden triterpen yapısında olan ursolik asit ve diterpen yapısında olan 6,7-dehidroroyleanon, asetil royleanon, ferruginol ve kriptanol bileşiklerinin yüksek bir sitotoksik potansiyele sahip olduğu belirlenmiştir. Ayrıca ilk defa tarafımızdan saflaştırılıp yapısı tayin edilen 5 bileşik ise orta derece antioksidan aktivite gösterirken çok yüksek sitotoksik etki gösterdikleri belirlenmiştir. Western Blot sonuçlarına göre çalışılan 5 türün de majör bileşiği olan rozmarinik asidin MCF-7 ve HT-29 hücre serisi üzerinde, metot validasyonu için standart olarak alınan kriptotanşinon bileşiğinin ise kanserli MCF-7 hücre serisi üzerinde umut verici sonuçlar verdiği görülmektedir.
  • Küçük Resim
    Öğe
    Bioguided isolation of secondary metabolites from Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa
    (ACG Publications, 2021) Ertaş, Abdulselam; Çakırca, Hatice; Yener, İsmail; Akdeniz, Mehmet; Fırat, Mehmet; Topçu, Gülaçtı; Kolak, Ufuk
    Abstract: In the current study, the ethanol extracts prepared from the aerial parts and roots of an endemic species, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and tested for determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, and cupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolated from the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4- hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2- dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone (11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid (15), oleanolic acid (16), taraxasterol (17), lupenone (18), β-sitosterol (19), and stigmasterol (20). Rosmarinic acid, which was obtained from the aerial parts, was found to be the best antioxidant compound among the isolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays (IC50: 1.20±0.04 µg/mL, IC50: 1.74±0.06 µg/mL, A0.5: 1.22±0.02 µg/mL, respectively). Chlorogenic and caffeic acids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidant activity in the mentioned assays.
  • [ X ]
    Öğe
    Bioguided Isolation of Secondary Metabolites fromSalvia cerino-pruinosa Rech. f. var. cerino-pruinosa
    (2021) Kolak, Ufuk; Ertas, Abdulselam; Firat, Mehmet; Cakirca, Hatice; Topcu, Gulacti; Yener, Ismail; Akdeniz, Mehmet
    In the current study, the ethanol extracts prepared from the aerial parts and roots of an endemicspecies, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and testedfor determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, andcupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolatedfrom the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4- hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2- dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone(11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid(15), oleanolic acid (16), taraxasterol (17), lupenone (18), ?-sitosterol (19), and stigmasterol (20). Rosmarinicacid, which was obtained from the aerial parts, was found to be the best antioxidant compound among theisolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays(IC50: 1.20±0.04 ?g/mL, IC50: 1.74±0.06 ?g/mL, A0.5: 1.22±0.02 ?g/mL, respectively). Chlorogenic and caffeicacids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidantactivity in the mentioned assays.
  • Küçük Resim
    Öğe
    Biological and chemical comparison of natural and cultivated samples of satureja macrantha C.A.Mey
    (ACG Publications, 2021) Akdeniz, Mehmet; Yener, İsmail; Ertaş, Abdulselam; Fırat, Mehmet; Reşitoğlu, Barış; Haşimi, Nesrin; Kandemir, Sevgi İrtegün; Yılmaz, Mustafa Abdullah; Demirköz, Aslı Barla; Kolak, Ufuk; Öksuz, Sevil
    Abstract: In this study, investigation on the essential oils and ethanol extracts of naturally grown and cultivated Satureja macrantha samples were reported. The essential oil, flavour and terpenoid-steroid-flavonoid contents of S. macrantha samples were determined by GC-MS and their phenolic contents by LC-MS/MS. Besides, the biological activities of the samples were investigated for their antioxidant, anti-Alzheimer, antimicrobial, cytotoxic, antityrosinase, antiurease, antielastase and anticollagenase properties. The phenolic content and antioxidant capacity of the cultivated sample were higher than those of the naturally grown sample. According to the GC-MS results, terpinene-4-ol (30.9%) and p-cymene (56.7%) were determined as the major components in the essential oils of the naturally grown and cultivated S. macrantha, respectively. The flavour analysis results showed that cis-sabinene hydrate (20.7%) and carvacrol (42.2%) were found to be the major components in the naturally grown and cultivated samples, respectively. While the naturally grown sample was rich in abietane diterpenoids (ferruginol (17.5 mg analyte/g extract) and sugiol (4.2 mg analyte/g extract)), these components were not detected in the cultivated sample. The rosmarinic acid content (0.20 and 24.87 mg analyte/g extract, respectively) of the cultivated sample was found to be significantly higher than that of the natural sample. The biological activities of the samples were determined to be changed in parallel with their chemical contents that are due to factors such as climatic conditions, and soil structure.
  • [ X ]
    Öğe
    Chemical Composition of The Essential Oils of Three Centaurea Species Growing Wild in Anatolia and Their Anticholinesterase Activities
    (Taylor & Francis Ltd, 2014) Ertas, Abdulselam; Goren, Ahmet Ceyhan; Boga, Mehmet; Demirci, Serpil; Kolak, Ufuk
    This report represents the first study on the chemical composition of essential oil of endemic Centaurea lycopifolia. This report also represents the first study on the anticholinesterase activity of essential oils of C. lycopifolia, C. balsamita and C. iberica. Essential oils were obtained using a Clevenger apparatus by hydrodistillation from the whole parts of C. lycopifolia, C. balsamita and C. iberica. The essential oils composition of the plants were determined by GC-FID and GC-MS (gas chromatograph/mass spectrometry) analysis. The major component of the essential oils was identified as caryophyllene oxide (9.7 %) and spathulenol (7.3 %) for C. lycopifolia, alpha-selinene (8.5 %) and hexatriacontane (8.3 %) for C. balsamita and arachidic acid (25.3 %) and hexadecanoic acid (5.9 %) for C. iberica. The essential oils of three Centaurea species indicated moderate inhibitory effect against butyrylcholinesterase and acetylcholinesterase enzyme, at 200 mu g/mL.
  • [ X ]
    Öğe
    Chemical compositions by LC-MS/MS and GC-MS and biological activities of Chenopodium album subsp. album var. microphyllum
    (Elsevier, 2019) Yilmaz, Pelin Koseoglu; Ertas, Abdulselam; Akdeniz, Mehmet; Avci, Mine Kocyigit; Kolak, Ufuk
    Chenopodium species have been used in folk medicine and as vegetable for years. In the present study, the total phenolic and flavonoid contents, biological activities, phenolic constituents and fatty acid profile of Chenopodium album subsp. album var. microphyllum (Boen.) Aellen were determined for the first time. The antioxidant effects were investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and cupric ion reducing antioxidant capacity assays (CUPRAC). The Ellman method was applied for the determination of the cholinesterase inhibition activity. The phenolic constituents of the methanol extract and the fatty acid profile of the n-hexane extract were evaluated by LC-MS/MS and GC-MS, respectively. Acetone and methanol extracts showed similar DPPH free radical scavenging activities (0.68 +/- 0.07 and 0.68 +/- 0.05 mmol Trolox/g extract, respectively), whereas the cupric ion reducing capacity of the acetone extract was the highest (0.41 +/- 0.05 mmol Trolox/g extract). Acetone and methanol extracts had moderate butyrylcholinesterase inhibitory activities as 65.29 +/- 1.56% and 52.64 +/- 2.78%, respectively, whereas non of the extracts possessed anti-acetylcholinesterase effect. The methanol extract was found to contain significant amounts of hesperidin (9769.13 +/- 158.26 mu g/g extract) and rutin (2935.19 +/- 39.92 mu g/g extract). The major fatty acid constituents of the n-hexane extract were identified as myristic acid (18.26%) and cis-10-pentadecanoic acid (15.39%).
  • [ X ]
    Öğe
    Chemical Constituents and Biological Activities of Cirsium leucopsis, C. sipyleum, and C. eriophorum
    (Walter De Gruyter Gmbh, 2014) Boga, Mehmet; Yilmaz, Pelin Koseoglu; Cebe, Deniz Baris; Fatima, Mashhad; Siddiqui, Bina S.; Kolak, Ufuk
    Two endemic Cirsium species, C. leucopsis DC. and C. sipyleum O. Schwarz, and C. eriophorum (L.) Scop. growing in Turkey were investigated to establish their secondary metabolites, fatty acid compositions, and antioxidant and anticholinesterase potentials. Spectroscopic methods were used to elucidate the structures of thirteen known compounds (p-hydroxy-benzoic acid, vanillic acid, cis-epoxyconiferyl alcohol, syringin, balanophonin, 1'-O-methyl-balanophonin, apigenin, kaempferol-3-O-beta-D-glucopyranoside, kaempferol-3-O-alpha-L-rhamnopyranoside, taraxasterol, taraxasterol acetate, beta-sitosterol, beta-sitosterol-3-O-beta-D-glucopyranoside). cis-Epoxyconiferyl alcohol and 1'-O-methyl-balanophonin were isolated for the first time from Cirsium species. Palmitic acid (47.1%) was found to be the main fatty acid of C. leucopsis, linoleic acid in both C. sipyleum (42.1%) and C. eriophorum (37.8%). Assays of beta-carotene bleaching, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) cation radicals, and superoxide anion radicals, as well as cupric reducing antioxidant capacity (CUPRAC) were used to determine the antioxidant activities of the extracts and isolated compounds. Vanillic acid, balanophonin, and kaempferol-3-O-alpha-L-rhamnopyranoside exhibited strong antioxidant activity. Taraxasterol was a potent inhibitor of acetyl- and butyrylcholinesterase activity, respectively.
  • [ X ]
    Öğe
    Diyarbakır çevresinde yetişen endemik ajuga vestita ekstresinin sekonder metabolitlerinin ve bazı biyolojik aktivitelerinin belirlenmesi
    (2012) Haşimci, Nesrin; Kolak, Ufuk; Tolan, Veysel
    [Abstract Not Available]
  • [ X ]
    Öğe
    Essential oil compositions and anticholinesterase activities of two edible plants Tragopogon latifolius var. angustifolius and Lycopsis orientalis
    (Taylor & Francis Ltd, 2014) Ertas, Abdulselam; Goren, Ahmet C.; Boga, Mehmet; Yesil, Yeter; Kolak, Ufuk
    This is the first report in the literature on essential oil compositions of Tragopogon latifolius var. angustifolius and Lycopsis orientalis which were analysed by using GC-FID and GC-MS techniques. The main constituents of T. latifolius var. angustifolius were identified as alpha-selinene (10.5%), 2,5-di-tert octyl-p-benzoquinone (9.5%) and valencene (7.0%); however, the main components of L. orientalis were identified as heptacosane (10.5%), tau-muurolene (9.6%) and tetratetracontane (9.4%). The essential oils of T. latifolius var. angustifolius and L. orientalis species exhibited moderate inhibitory activity against acetyl- and butyryl-cholinesterase enzymes at 200 mu g/mL.
  • Küçük Resim
    Öğe
    Essential oil contents of Hypericum linarioides , H. helianthemoides, and H. lydium with their biological activities: Importance of Hypericum genus in the cosmeceutical and pharmaceutical industries
    (Springer, 2023) Akdeniz, Mehmet; Yener, İsmail; Ertaş, Abdülselam; Dincel, Demet; Fırat, Mehmet; Kocakaya, Şafak Özhan; Yigitkan, Serkan; Türkmenoğlu, Fatma Pınar; Aydın, Fırat; Kolak, Ufuk
    In the literature, more than 3000 studies have been carried out on species in the Hypericum genus in the last ten years, especially for H. perforatum. The importance of Hypericum species in traditional medicine and modern medicine is increasing day by day. From this point of view, the chemical composition of H. linarioides, H. helianthemoides, and H. lydium essential oils was analyzed by GC-MS/FID. In addition, their antioxidant, cytotoxic and antimicrobial activities along with their enzyme inhibitory potentials (cholinesterase, urease, tyrosinase, elastase and, collagenase) were defined. Also, in vitro and in silico studies of the major components of the species have been carried out on the studied enzymes. H. linarioides and H. helianthemoides were found to be rich in monoterpene hydrocarbons (63.96% and 43.86%, respectively), and H. lydium in sesquiterpene hydrocarbons (48.41%). The major components were indicated as α-pinene for H. linarioides (50.39%) and for H. helianthemoides (36.84%), and β-selinene for H. lydium (10.12%). H. lydium essential oil exhibited very high cytotoxic activity on breast cancer (MCF-7) (selectivity indices SI: 12) and colon cancer (HT-29) (SI: 2) cell lines. In particular, the selectivity indice value in MCF-7 cell lines of H. lydium could have natural therapeutic potential for breast cancer. H. lydium possessed neuropharmacological potential due to its high anticholinesterase activity. Since H. linarioides indicated high anti-aging potential (tyrosinase, elastase, and collagenase enzyme inhibitory activities), it could be used in the cosmetic industry.
  • Küçük Resim
    Öğe
    Essential oil, aroma, and fatty acid profiles of five endemic Salvia taxa from Turkey with chemometric analysis
    (Wiley-V C H Verlag GMBH, 2022) Ertaş, Abdulselam; Akdeniz, Mehmet; Yener, İsmail; Öztürk, Mehmet; Ölmez, Özge Tokul; Fırat, Mehmet; Kolak, Ufuk
    The essential oil, aroma, and fatty acid compositions of Salvia cerino-pruinosa var. cerino-pruinosa, S. cerino-pruinosa var. elazigensis, S. pseudeuphratica, S. rosifolia, and S. kurdica collected in 2015, 2016, and 2017 were analysed by GC-FID, GC/MS/Head Space and GC/MS. The results were evaluated chemometrically for principal component analyses and hierarchical clustering analyses using Minitab program. The main components of their essential oils have been determined as germacrene-D (41.79 % highest for these species), camphor (55.80 %), linalool (33.38 %), caryophyllene oxide (32.99 %), 1,8-cineole (26.30 %) and geraniol acetate (31.63 %), while for aroma 1,8-cineole (45.95 % highest for these species), camphor (58.54 %), D-limonene (40.83 %), linalool (27.67 %) and cis-linalool oxide (25.87 %). Oleic (72.65 % highest for these species), erucic (44.61 %), 2-palmitoleic (42.17 %) and nervonic (32.61 %) acids were determined as major components of their fatty acids. According to the PCA and HCA, the essential oil components of these Salvia taxa unaffected by the years, and accordingly, the Salvia taxa grouped among themselves.
  • [ X ]
    Öğe
    Evaluation of Antioxidant, Cholinesterase Inhibitory and Antimicrobial Properties of Mentha longifolia subsp noeana and Its Secondary Metabolites
    (Acg Publications, 2015) Ertas, Abdulselam; Goeren, Ahmet C.; Hasimi, Nesrin; Tolan, Veysel; Kolak, Ufuk
    The aim of the present study was to determine the chemical structures of the isolated compounds, the essential oil and fatty acid compositions of Mentha longifolia subsp. noeana with their biological activities. Ursolic acid (1), uvaol (2), stigmast-5-ene-3 beta-yl formate (3), stigmast-5-en-3-one (4), beta-sitosterol (5), bis(2ethylhexyl) benzene-1,2-dicarboxylate (6), hexacosyl (E)-ferulate (7) and 5-hydroxy-6,7,3',4'-tetramethoxy flavone (8) were obtained from the aerial parts. The compounds (2-4, 6, 7) were isolated for the first time from a Mentha species. Palmitic acid (40.8%) was the major component of the non-polar fraction obtained from the petroleum ether extract. Pulegone (32.3%) was the main constituent of the essential oil which exhibited strong butyrylcholinesterase inhibitory activity (77.36 +/- 0.29%), moderate antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. The methanol extract showed 80% inhibition of lipid peroxidation, and the acetone extract possessed moderate DPPH free radical scavenging activity (60% inhibition) at 100 mu g/mL.
  • [ X ]
    Öğe
    A GC-MS method validation for quantitative investigation of some chemical markers inSalvia hypargeiaFisch. & CA Mey. of Turkey: Enzyme inhibitory potential of ferruginol
    (Wiley, 2020) Bakir, Derya; Akdeniz, Mehmet; Ertas, Abdulselam; Yilmaz, Mustafa A.; Yener, Ismail; Firat, Mehmet; Kolak, Ufuk
    In the present study, a GC-MS method used for quantitative screening of 26 compounds (sclareolide, sclareol, ferruginol, cryptanol, 6,7-dehydroroyleanone, suginal, 9,10-dihydro-7,8-dimethyl-2-(1-methylethyl) phenanthren-3-ol, sugiol, inuroyleanone, 12-demethylmulticauline, 7 alpha-hydroxy-beta-sitosterol, stigmasterol, sitosterol, salvigenin, sinensetin,alpha-amyrin, lupeol, lupenone, 3-acetyl lupeol, 1 alpha,21 alpha-dihydroxy-2,3-(1 ' 1 '-dimethyl-dioxymethylene) urs-9(11),12-dien, uvaol, betulin, pyxinol, lup-(20),29-ene-2 alpha-hydroxy-3 beta-acetate, betulin 3 beta, 28 beta-diacetate, 21 alpha-hydroxy,2 alpha,3 beta-diacetoxy urs-9(11),12-dien) specific to TurkishSalviaspecies was developed and validated. According to the GC-MS analysis results,Salvia hypargeiaFisch. & C.A. Mey. roots were found to be rich in ferruginol (30787.97 mu g/g extract) and lupenone (23276.21 mu g/g extract), and leaves in lupeol (20625.92 mu g/g extract). Additionally, the essential oil and aroma contents of this species were identified by GC-MS technique. According to the LC-MS/MS results, especiallyS. hypargeialeaf extract was rich in rosmarinic acid (38035.7 mu g/g extract) and isoquercitrin (4136.91 mu g/g extract). Furthermore, anticholinesterase, antiurease, antityrosinase and antielastase inhibitory, antioxidant, cytotoxic activities of the ethanol extracts, essential oil, and major components of the species were evaluated. Antioxidant potentials of all extracts of this species were quite high in all studied antioxidant methods. Moreover, butyrylcholinesterase and elastase inhibitory capacities of ferruginol, the major component ofS. hypargeiaroots, were notable. For these reasons, this species has a high potential for food and pharmaceutical industries. Practical applications This new GC-MS method was applied toS. hypargeiaFisch. & C.A. Mey. and it indicated that this species possessed high amount of ferruginol and lupeol, and that this species could be used for their natural sources. According to the results of the activity studies (antioxidant, anticholinesterase, tyrosinase, elastase, and cytotoxic), this method was used to exhibit which compound may be responsible for the activities. This developed and validated method could be easily applied to determine major/active/toxic secondary metabolites ofSalviaspecies which are used and/or could be used in pharmaceutical, cosmetic, and food industries.
  • [ X ]
    Öğe
    In vitro biological activities and fatty acid profiles of Pistacia terebinthus fruits and Pistacia khinjuk seeds
    (Taylor & Francis Ltd, 2015) Hacibekiroglu, Isil; Yilmaz, Pelin Koseoglu; Hasimi, Nesrin; Kilinc, Ersin; Tolan, Veysel; Kolak, Ufuk
    This study reports in vitro anticholinesterase, antioxidant and antimicrobial effects of the n-hexane, dichloromethane, ethanol and ethanol-water extracts prepared from Pistacia terebinthus L. fruits and Pistacia khinjuk Stocks seeds as well as their total phenolic and flavonoid contents, and fatty acid compositions. Ethanol and ethanol-water extracts of both species exhibited higher anticholinesterase activity than galanthamine. Among ABTS, DPPH and CUPRAC assays, the highest antioxidant capacity of the extracts was found in the last one. P. terebinthus ethanol extract being rich in flavonoid content showed the best cupric reducing effect. All extracts possessed no antimicrobial activity. The main fatty acid in P. terebinthus fruits (52.52%) and P. khinjuk seeds (59.44%) was found to be oleic acid. Our results indicate that P. terebinthus fruits and P. khinjuk seeds could be a good source of anticholinesterase compounds, and could be phytochemically investigated.
  • Küçük Resim
    Öğe
    Investigation of cytotoxic and apoptotic effects of 63 compounds obtained from Salvia species: Promising anticancer agents
    (Wiley, 2022) Kandemir, Sevgi İrtegün; Fidan, Hilal Saruhan; Yener, İsmail; Mete, Nuriye; Ertaş, Abdulselam; Topçu, Gülaçtı; Kolak, Ufuk
    Since ancient time, Salvia L. species have been commonly used to treat colds, bronchitis, tuberculosis, heart diseases, and menstrual and digestive disorders in traditional medicine all around the world. They have been also used as tea and spice. Studies indicated that diterpenes and triterpenes isolated from Salvia species possess various pharmacological and biological effects such as anti-inflammatory, antiviral, cytotoxic, antioxidant, and hepatotoxic activities. Flavones were also shown to have antimicrobial, antioxidant, and cytotoxic potentials. Salvia extracts also exhibit anti-Alzheimer, antiseptic, cardiovascular, antihypertensive, and antituberculous effects. To investigate the effects of 63 secondary metabolites from Salvia species on cell viability and apoptosis, Salvia secondary metabolites including 25 phenolics, 4 fatty acids, 19 abietane diterpenoids, 12 triterpenoids, and three steroids were examined on healthy cell line (PDF), breast cancer (MCF-7), and colon cancer (HT-29) cell lines using MTT method. In addition, the effects of rosmarinic acid, 6,7-dehydroroyleanone, acetyl royleanone, ferruginol, carnosic acid, carnosol, cryptotanshinone, beta-sitosterol, and ursolic acid on pro-apoptotic Bax and antiapoptotic Bcl-2 protein expression levels were investigated by Western Blot method. Practical applications Phenolic compounds (apigenin, chrysin, and luteolin) and diterpenes (especially dihydrotanshinone I, carnosic acid, and carnosol), and almost all of the triterpenes exhibited high toxic effects on healthy cell line. Cytotoxic effects of cryptotanshinone, 12-hydroxy abieta-1,3,5(10),8,11,13-hexaene, 12-demethylmulticauline, 6,7-dehydroroyleanone, acetyl royleanone, ferruginol, ursolic acid, and 3-acetyl lupeol were relatively higher than their toxic effects. Acetyl royleanone, 6,7-dehydroroyleanone, carnosic acid, and cryptotanshinone were found to have anticancer potential based on their modulating effects on the expression levels of Bax and Bcl-2 proteins which play important roles in the regulation of apoptosis. The results of the present study showed that acetyl royleanone, cryptotanshinone, 6,7-dehydroroyleanone, carnosic acid, and cryptotanshinone have potential to be used in the pharmaceutical industry
  • [ X ]
    Öğe
    LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities
    (2017) Özden, Tuğba Yılmaz; Haşimi, Nesrin; Boğa, Mehmet; Yılmaz, Mustafa Abdullah; Kolak, Ufuk; Kayıran, Serpil Demirci; Temel, Hamdi
    Bu çalışmada, Anadolu'da yetişen üç endemik Astragalus türünün kimyasal içeriği ile bazı biyolojik aktivitelerinin belirlenmesi hedeflenmiştir. Astragalus leporinus var. hirsutus, Astragalus distinctissimus ve Astragalus schizopterus türlerinin kimyasal içeriği LC-MS/MS ve GC-MS ile karakterize edilmiştir. Biyolojik aktivite açısından; ekstrelerin antioksidan, antikolinesteraz, antimikrobiyal ve sitotoksik aktiviteleri tespit edilmiştir. Ek olarak, ekstrelerin ana bileşenlerinin de antioksidan özellikleri belirlenerek elde edilen sonuçlar ekstrelerin antioksidan kapasiteleri ile karşılaştırılmıştır. Çalışılan Astragalus türlerinde en bol bulunan flavonoidin rutin (1028,276-13351,76 µg/g ekstre) ve hesperidin (1604,348-9695,435 µg/g ekstre) olduğu, ayrıca A. schizopterus metanol ekstresinde de yüksek miktarda kuinik asit (111302,774 µg/g ekstre) bulunduğu belirlenmiştir. Palmitik asitin (C16:0), A. leporinus var. hirsutus (%32,9), A. distinctissimus (%32,5) ve A.schizopterus (%23,4)'da başlıca bileşik olduğu saptanmıştır. A. schizopterus metanol ekstresi lipit peroksidasyon (19,62±0,29), DPPH serbest radikal (54,61±0,38) , ABTS katyon radikali süpürücü aktivitesi (22,01±0,07), ve CUPRAC yönteminde en yüksek aktiviteyi göstermiştir. Çalışılan tüm ekstrelerden sadece A. leporinus var. hirsutus petrol eteri ekstresi orta derecede asetil- ve bütiril- kolinesteraz enzim inhibisyonu sergilemiştir.. Bitkilerin metanol ekstreleri C. albicans'a karşı orta derecede antimikrobiyal aktivite ortaya koymuşlardır. A. leporinus var. hirsutus metanol ekstresi, L929 fibroblast hücrelerine karşı en fazla canlılığı ve A549 hücrelerine karşı en yüksek sitotoksik etkiyi göstermiştir. Çalışmdan elde ettiğimiz bulgular ışığında, hayvan yemi olarak kullanılan bu Astragalus türleri, gıda ve ilaç endüstrisinde kullanılabilen doğal, biyolojik olarak aktif maddeler kaynağı olabilir.
  • [ X ]
    Öğe
    Method validation of 15 phytochemicals inHypericum lysimachioidesvar.spathulatumby LC-MS/MS, and fatty acid, essential oil, and aroma profiles with biological activities
    (Springer, 2020) Akdeniz, Mehmet; Yilmaz, Mustafa Abdullah; Ertas, Abdulselam; Yener, Ismail; Firat, Mehmet; Aydin, Firat; Kolak, Ufuk
    The aim of the present study was to develop and validate a LC-MS/MS method for quantification of 15 phytochemicals inHypericumspecies. The developed method was fully validated in terms of repeatability (inter-day and intra-day precision), limits of detection and quantification, linearity, recovery and relative standard uncertainty. The developed and validated LC-MS/MS method was applied to determine 15 phytochemicals in the ethanol extracts ofH. lysimachioidesvar.spathulatumaerial parts (HLS-A) and roots (HLS-R). Hyperoside was found to be the major compound in HLS-A and HLS-R ethanol extracts (16,560.3 and 3561.6 mu g analyte/g extract, respectively). According to the results of GC-MS analyses,cis-13,16-docosadienoic acid (35.0%), caryophyllene oxide (24.33%) and undecane (28.21%) were determined as the major components in fatty acid, essential oil and aroma compositions ofH. lysimachioidesvar.spathulatum, respectively. HLS-A and HLS-R ethanol extracts showed moderate activity in ABTS cation radical decolorization assay. The major components (hyperoside, astragalin, and quercetin) present in HLS-A and HLS-R ethanol extracts were found to have also the highest antioxidant effect in ABTS cation radical scavenging method. The extracts, main constituents in the extracts and essential oil had no toxic-cytotoxic potential against PDF, MCF-7 and HT-29 cell lines. HLS-A ethanol extract and essential oil exhibited high butyrylcholinesterase inhibitory activity. Quercetin showed the highest inhibitory effect against acetyl- and butyryl-cholinesterase, urease and tyrosinase among the tested samples. According to biological activity studies,H. lysimachioidesvar.spathulatumand their major components might be promoted as promising sources of natural agents and used in the development of nutraceuticals, functional food ingredients and pharmaceutical industry.
  • [ X ]
    Öğe
    Phenolic Profile, Fatty Acid and Essential Oil Composition Analysis and Antioxidant, Antialzheimer and Antibacterial Activities of Verbascum flavidum Extracts
    (Chiang Mai Univ, 2016) Boga, Mehmet; Ertas, Abdulselam; Hasimi, Nesrin; Demirci, Serpil; Yilmaz, Mustafa Abdullah; Temel, Hamdi; Kolak, Ufuk
    This paper is the first report about phytochemical analysis with LC-MS/MS and biological activities of Verbascum flavidum (Boiss.) Freyn & Bornm. Rutin (1077.09 +/- 77.47 mu g analyte/g extract) and chlorogenic acid (1012.42 +/- 97.18 mu g analyte/g extract) were determined as the most abundant phenolics in V. flavidum. The main components of fatty acid were found to be palmitic (30.3%) and oleic acids (17.9%); while the major essential oils were determined as arachidic acid (16.4%) and alpha-selinene (8.9%) by GC-MS analysis. In DPPH free radical scavenging activity the methanol extract showed higher activity than BHT with 72.62% inhibition at 100 mu g/mL concentration. The methanol and water extracts of V. flavidum showed 86.01 and 87.39 % inhibition in ABTS cation radical scavenging assay at 100 mu g/mL concentration, respectively. The methanol extract showed moderate activity against E. coli with 250 +/- 0.3 MIC value and with 15 mm inhibition zone diameter at 30 mg/mL concentration.