A GC-MS method validation for quantitative investigation of some chemical markers inSalvia hypargeiaFisch. & CA Mey. of Turkey: Enzyme inhibitory potential of ferruginol
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Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In the present study, a GC-MS method used for quantitative screening of 26 compounds (sclareolide, sclareol, ferruginol, cryptanol, 6,7-dehydroroyleanone, suginal, 9,10-dihydro-7,8-dimethyl-2-(1-methylethyl) phenanthren-3-ol, sugiol, inuroyleanone, 12-demethylmulticauline, 7 alpha-hydroxy-beta-sitosterol, stigmasterol, sitosterol, salvigenin, sinensetin,alpha-amyrin, lupeol, lupenone, 3-acetyl lupeol, 1 alpha,21 alpha-dihydroxy-2,3-(1 ' 1 '-dimethyl-dioxymethylene) urs-9(11),12-dien, uvaol, betulin, pyxinol, lup-(20),29-ene-2 alpha-hydroxy-3 beta-acetate, betulin 3 beta, 28 beta-diacetate, 21 alpha-hydroxy,2 alpha,3 beta-diacetoxy urs-9(11),12-dien) specific to TurkishSalviaspecies was developed and validated. According to the GC-MS analysis results,Salvia hypargeiaFisch. & C.A. Mey. roots were found to be rich in ferruginol (30787.97 mu g/g extract) and lupenone (23276.21 mu g/g extract), and leaves in lupeol (20625.92 mu g/g extract). Additionally, the essential oil and aroma contents of this species were identified by GC-MS technique. According to the LC-MS/MS results, especiallyS. hypargeialeaf extract was rich in rosmarinic acid (38035.7 mu g/g extract) and isoquercitrin (4136.91 mu g/g extract). Furthermore, anticholinesterase, antiurease, antityrosinase and antielastase inhibitory, antioxidant, cytotoxic activities of the ethanol extracts, essential oil, and major components of the species were evaluated. Antioxidant potentials of all extracts of this species were quite high in all studied antioxidant methods. Moreover, butyrylcholinesterase and elastase inhibitory capacities of ferruginol, the major component ofS. hypargeiaroots, were notable. For these reasons, this species has a high potential for food and pharmaceutical industries. Practical applications This new GC-MS method was applied toS. hypargeiaFisch. & C.A. Mey. and it indicated that this species possessed high amount of ferruginol and lupeol, and that this species could be used for their natural sources. According to the results of the activity studies (antioxidant, anticholinesterase, tyrosinase, elastase, and cytotoxic), this method was used to exhibit which compound may be responsible for the activities. This developed and validated method could be easily applied to determine major/active/toxic secondary metabolites ofSalviaspecies which are used and/or could be used in pharmaceutical, cosmetic, and food industries.
Açıklama
Anahtar Kelimeler
Essential Oil, Ferruginol, Gc-Ms Method Validation, Lc-Ms, Ms, Salvia Hypargeia, Terpenoid
Kaynak
Journal of Food Biochemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
44
Sayı
9
