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Öğe Effect of Vernal Keratoconjunctivitis on Vitreous Depth in Patients with Keratoconus(Informa Healthcare, 2015) Cingu, Abdullah Kursat; Kaya, Savas; Cinar, Yasin; Sahin, Muhammed; Turkcu, Fatih Mehmet; Yuksel, Harun; Murat, MehmetPurpose: The aim of this study was to comparatively evaluate the vitreous depth (VD) of keratoconic eyes in patients with or without vernal keratoconjunctivitis (VKC). Material and Methods: Eighty eyes of 80 consecutive keratoconus (KC) patients and 40 emmetropic control subjects were enrolled. KC patients were divided into two groups according to accompanying VKC (VKC-KC group and KC group). Mean outcome measures were best-corrected visual acuity (BCVA), spherical equivalent (SE), mean keratometry (Km), intraocular pressure (IOP), and axial length (AL) and its components. The eyes with the highest Km were selected for statistical analysis for each participant. Results: There were 50, 30, and 40 patients in the VKC-KC group, KC group, and control group respectively. The KC group and VKC-KC group were similar in BCVA, SE, Km, CCT, ACD, LT, and IOP (p>0.05). The mean ACD was significantly lower in the control group when compared with the KC group and VKC-KC group. The mean AL and VD were significantly higher in VKC-KC group than those of KC group and the control group, whereas similar in KC and control groups (p>0.05). Conclusion: In the current study we showed that VKC-associated KC patients have significantly longer AL and VD when compared with KC patients without VKC. Posterior segment elongation in VKC-KC group may be associated with the type IV collagen destruction due to chronic longstanding inflammation in VKC patients.Öğe The effects of caffeic acid phenethyl ester in acute methanol toxicity on rat retina and optic nerve(Taylor & Francis Ltd, 2013) Sahin, Alparslan; Kaya, Savas; Turkcu, Gul; Cingu, Abdullah Kursat; Yuksel, Harun; Turkcu, Fatih Mehmet; Yuksel, HaticePurpose: We aimed to test caffeic acid phenethyl ester (CAPE) as an antidote for acute methanol (MeOH) toxicity and to compare it with ethanol. Methods: This study included five groups, each containing eight rats. The groups were control, methotrexate (MTX), MeOH, ethanol and CAPE. All rats except control group were treated with intraperitoneal (i.p.) MTX (0.3 mg/kg/d) for 7 d. At the 8th day of the experiment, i.p. injection of MeOH (3 g/kg) was administered in MeOH, ethanol and CAPE groups. Four hours after MeOH treatment, 0.5 g/kg ethanol was injected i.p. in ethanol group; 10 mu mol/kg CAPE i.p. in CAPE group; serum physiologic i.p. in other groups. After 8 h, rats were anaesthetized and sacrificed. Total anti-oxidant status (TAS), total oxidant status (TOS) were measured on the dissected and excised retina and optic nerve samples. Fellow eyes were used for histopathologic evaluation and the cell count of retinal ganglion cell (RGC) layer. In addition, interactions of alcohol dehydrogenase with CAPE, ethanol, MeOH and pyrazole derivatives were investigated. Results: Either CAPE or ethanol co-treatment decreased the TOS levels and increased the TAS levels compared to the MeOH group. MeOH treatment decreased the mean cell count in RGC layer. CAPE co-treatment significantly prevented cell loss (p=0.040). Besides, in silico calculations showed that binding affinity of CAPE to alcohol dehydrogenase was higher than those of MeOH, ethanol, and pyrazole derivatives were. Conclusion: This study demonstrated that CAPE treatment decreased the oxidative stress in acute MeOH intoxication in the retina and optic nerve; beside that, protected RGC layer histology. In silico, CAPE had higher affinity score than MeOH, ethanol, pyrazole and pyrazole derivatives in the case of interaction with alcohol dehydrogenase.Öğe The effects of caffeic acid phenethyl ester on retina in a diabetic rat model(Taylor & Francis Ltd, 2021) Sahin, Alparslan; Kaya, Savas; Baylan, MukadderPurpose We aimed to investigate the effect of caffeic acid phenethyl ester (CAPE) on retinal apoptosis and oxidative stress parameters in streptozotocin (STZ) induced diabetic rat model. Methods This study included 3 groups; control, STZ, and STZ + CAPE. The rats in STZ, and STZ + CAPE groups were injected with STZ (35 mg/kg, i.p.) for induction of diabetes. In the STZ + CAPE group, 10 mu mol/kg of CAPE were intraperitoneally injected for 4 weeks. Control and STZ groups were given only intraperitoneal vehicle (saline). Rats were anaesthetized and sacrificed on the 4th week of the experiment. Total anti-oxidant status (TAS), and total oxidant status (TOS) were measured on the dissected retinal tissues. Oxidative stress index (OSI) was also calculated. Fellow eyes were used for histopathologic evaluation with caspase-3 and matrix metalloproteinase-2 (MMP-2) and MMP-9 evaluation. Results TAS levels were similar between groups (p = 0.71). However, CAPE treatment prevented the elevation of the TOS in the STZ + CAPE group compared to the STZ group (30.93 +/- 9.97 vs 61.53 +/- 24.7 nmol H2O2 Eq/mg protein, p = 0.007). OSI was also significantly lower in the STZ + CAPE group than that of the STZ group (20.01 +/- 5.87 vs. 37.90 +/- 14.32, respectively, p = 0.007). Retinal caspase-3 staining, MMP-2 and MMP-9 scores were not different between groups (p > 0.05 for all). Conclusion The present study demonstrated that CAPE treatment may decrease the oxidative stress in the retina in STZ induced diabetic rat model. However, apoptosis was not observed in the retina. The retinal apoptosis cannot be shown probably due to a shorter period of diabetes.Öğe Evaluation of Corneal Parameters with Scheimpflug Imaging in Patients with Rheumatoid Arthritis(Informa Healthcare, 2013) Cingu, Abdullah Kursat; Cinar, Yasin; Turkcu, Fatih Mehmet; Sahin, Muhammed; Kaya, Savas; Bozkurt, Mehtap; Sahin, AlparslanPurpose: To evaluate corneal parameters of rheumatoid arthritis (RA) patients by Pentacam-HR. Methods: Seventy RA patients and 100 control subjects were enrolled. All participants underwent Pentacam (Pentacam-HR, Oculus, Germany) evaluation. Both RA and control groups were divided into two subgroups as dry eye (DE) (Schirmer test with topical anesthesia (STA) <= 5 mm) and without DE (STA > 5 mm). Results: Pachymetric measurements and the mean corneal volume were significantly lower in RA group (p<0.001). Disease duration was negatively correlated with pachymetric measurements in RA group. Pachymetric measurements and corneal volume of RA patients with DE were significantly lower than all the other subgroups. Control subgroups with or without DE were similar in pachymetric measurements and corneal volume. Conclusions: The results suggest that RA patients have thinner corneas compared to control subjects that may be affected by disease duration. Furthermore, coexistence of DE and RA seems to aggravate the thinning of cornea as well.Öğe Evaluation of the Anterior Segment Parameters of the Patients with Scleroderma(Taylor & Francis Inc, 2017) Sahin, Muhammed; Yuksel, Harun; Sahin, Alparslan; Cingue, Abdullah Kursat; Turkcu, Fatih Mehmet; Kaya, Savas; Yazmalar, LeventPurpose: To evaluate corneal parameters of scleroderma (SC) patients by Pentacam-HR. Methods: Twenty-two eyes of 22 SC patients and 33 eyes of 33 control subjects were enrolled. All participants underwent Pentacam (Pentacam-HR, Oculus, Germany) evaluation. Both SC and control groups were divided into two subgroups as dry eye (DE) (Schirmer test with topical anesthesia (STA) <5 mm) and without DE (STA >5 mm). Results: Pachymetric measurements and mean corneal volume (CV) were significantly lower in the SC group than in the control group (p<0.001). Pachymetric measurements and CV of SC patients with DE were significantly lower than all the other subgroups. Control subgroups with or without DE were similar in pachymetric measurements and CV. Conclusions: The results suggest that SC patients have thinner corneas compared with control subjects. Additionally, coexistence of DE seems to have an additional impact in the thinning of cornea in SC patients.Öğe Increased Caspase-3 Immunoreactivity of Erythrocytes in STZ Diabetic Rats(Hindawi Publishing Corporation, 2012) Firat, Ugur; Kaya, Savas; Cim, Abdullah; Buyukbayram, Huseyin; Gokalp, Osman; Dal, Mehmet Sinan; Tamer, Mehmet NumanEryptosis is a term to define apoptosis of erythrocytes. Oxidative stress and hyperglycemia, both of which exist in the diabetic intravascular environment, can trigger eryptosis of erythrocytes. In this experimental study, it is presented that the majority of erythrocytes shows caspase-3 immunoreactivity in streptozocin- (STZ)-induced diabetic rats. Besides that, caspase-3 positive erythrocytes are aggregated and attached to vascular endothelium. In conclusion, these results may start a debate that eryptosis could have a role in the diabetic complications.Öğe Intravenous Repeated Application of Low Dose ?-Galatosylceramide Boosts IFN-? Production in BALB/c Mice(Akad Doktorlar Yayinevi, 2012) Kaya, SavasThe aim of this study is to illustrate the relationship between Alpha Galatosylceramide (alpha-GalCer) dose and alpha-GalCer induced anergy of immune system in vivo. alpha-GalCer, a glycolipid and a widely studied immune modulator extracted from a marine sponge, shows its effect in vivo via presentation on CD1d molecule, MHC I like molecule bound glycolipid antigens, to NKT cells, a lymphocyte has both TCR and NK1.1, marker of T cells and NK cells respectively. The contradictory reports about alpha-GalCer are focused on two opposite axes of immune system: cancer and autoimmunity, failure and exaggeration of Th1 type immune response, respectively. In this study, the relation between the alpha-GalCer dose and the anergy was considered to clarify this dilemma. The groups of BALB/c mice were injected with various titer of alpha-GalCer intravenous way repeatedly and serum level of IFN-gamma was measured with mouse IFN-gamma ELISA. The Dramatic difference was found between groups after 2nd application of alpha-GalCer: Low dose alpha-GalCer boosted IFN-gamma; however, high dose, usual dose cited in literature, failed to induce IFN-gamma production. Besides, low concentrations of _-GalCer succeeded considerable amount of IFN-gamma in the serum after priming. This results show that minute level of alpha-GalCer can be recognized by NKT cells, and repeated in vivo application of low dose alpha-GalCer can improve Th1 capabilities of the immune system.Öğe Intravitreal bevacizumab monotherapy for retinopathy of prematurity(Wiley-Blackwell, 2013) Sahin, Alparslan; Sahin, Muhammed; Cingu, Abdullah Kuersat; Cinar, Yasin; Turkcu, Fatih Mehmet; Yuksel, Harun; Kaya, SavasBackgroundThe aim of this study was to evaluate the treatment outcomes of intravitreal bevacizumab (IVB) injections, used as a monotherapy in type 1 retinopathy of prematurity (ROP). MethodsA retrospective chart review was performed for 17 type 1 ROP patients (34 eyes), who had IVB injection between July 2011 and June 2012. Birthweight, gestational age at birth, stage and location of ROP, IVB injection time, time of complete retinal vascularization, and additional treatments if needed, were noted. A total of 0.625mg (0.025mL) bevacizumab was injected intravitreally. ResultsThirty eyes of 17 patients with type 1 ROP enrolled in the study were treated with IVB injection. Of them seven had aggressive posterior-ROP, six had stage 2 ROP, and four had stage 3 ROP. The mean gestational age was 28.44 weeks (range, 26-31 weeks); and the mean birthweight was 1151.88g (range, 600-1600g). The mean age for IVB injection was 35.47 weeks. The mean full retinal vascularization time was 136.6 26.6 days. The mean follow-up time was 285.3 +/- 70 days. ROP was regressed and retinal vascularization was completed in all cases except one eye, which had threshold disease. ConclusionIVB injection, used as a monotherapy, is an effective treatment approach in patients with type 1 ROP. Timely treatment of stage 2 and early stage 3 ROP in which disease progression was observed, prevents vitreoretinal membrane formation in posterior disease. Further studies need to be performed to determine the safety of IVB injection.Öğe Justification for the Use of CA 125 Levels After Cardiac Surgery(Akad Doktorlar Yayinevi, 2009) Peker, R. Oktay; Peker, Tuelay T.; Varol, Ercan; Ozaydin, Mehmet; Gulmen, Senol; Gokalp, Osman; Kaya, SavasCA 125, a tumor marker has been found to be elevated in malign conditions as well as in some benign conditions like heart failure. Cardiac surgery has been shown to cause a systemic inflammatory response. In this study, we investigated alterations in serum levels of CA 125 during cardiac surgery with cardiopulmonary by-pass. Thirty nine patients with a mean age of 54.7 years who underwent either coronary bypass surgery or valvular heart surgery were prospectively recruited to the study. We measured plasma levels of CA 125 preoperatively and on postoperative days I and 7. Comparing with preoperative values, CA 125 levels were similar on postoperative day 1(5.26 [6.89] U/ml vs 5.74 [4.54] U/ml) but elevated significantly on postoperative day 7 (42.1 [34] U/ml, p< 0.0001) (data in median interquantile range). CA 125 levels were found to be elevated after cardiac surgery. The elevations are more in patients undergoing valvular heart surgery than coronary bypass graft surgery. Although CA 125 can be considered a reliable tumor marker in the diagnosis and follow up of patients with malignant diseases, the presence of a recent cardiac surgery with cardiopulmonary bypass must be taken into account when asking for the cause of elevated CA 125 plasma level.Öğe Production of novel peptide-targeting antibodies for anti-Müllerian hormone receptor 2 and induction of cytotoxicity in ovarian cancer cells(Wiley, 2025) Sakalar, Cagri; Kurt, Busra; Sezen, Sedat; Kaya, SavasOvarian cancer is generally diagnosed at late stages. Monoclonal antibodies (MAbs) targeting antigens in ovarian cancer are used in the clinic. Anti-M & uuml;llerian hormone receptor type 2 (AMHR2) is a receptor highly expressed in ovarian cancer and it is a potential target antigen for immunotherapy. Extracellular domain of AMHR2 was analysed in terms of 3D structure and physicochemical properties, and 3 peptide sequences (Peptides 1, 7 and 11) were determined as targets. MAb production protocol was performed, and 6 MAb clones showing high affinity for peptides were obtained. P3B1, P10A10, P10B6 and P2A6 clones were for peptide 11 (P11), P2C9 was for P7, and P6C5 was for P1. Antibody isotype of P2A6 was IgG2a and the others were of IgG1 isotype. MAb binding to the native recombinant protein (AMHR2-Fc) was analysed by enzyme-linked immunosorbent assay (ELISA) and MAb binding to AMHR2 expressed by SKOV-3 ovarian cancer cells was analysed by western blot and immunofluorescent staining. P3B1 showed strong, P10A10, P10B6 and P2C9 showed medium affinity for the native protein (AMHR2-Fc). P3B1 and P2C9 showed strong binding in western blot analysis. Clones showed moderate binding in immunoflorescent staining. A complement dependent cytotoxicity (CDC) experiment was conducted using MAbs and transfected SKOV-3 cells. P3B1 induced a significant CDC. Variable regions of P3B1 MAb were sequenced. In conclusion, MAbs for three different regions of AMHR2 were produced. One clone was shown to induce cytotoxicity in ovarian cancer cells and its sequence was determined for future use as a humanised therapeutic MAb.Öğe The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells(Canadian Soc Clinical Investigation, 2013) Tufek, Adnan; Kaya, Savas; Tokgoz, Orhan; Firat, Ugur; Evliyaoglu, Osman; Celik, Feyzi; Karaman, HaktanPurpose: This study was designed to assess the correlation between the neuroprotective effect of dexmedetomidine and oxidative stress, neural inflammation and mast cell stability in rats with bupivacaine-induced sciatic nerve toxicity. Methods: Forty adult Wistar Albino rats, eight rats per group, were used. Saline (0.3 ml of 0.9%), dexmedetomidine (20 mu g/kg), 0.5% bupivacaine or 0.5% bupivacaine+dexmedetomidine (20 mu g/kg) was injected into the sciatic nerve. A control group of rats received no injection. Fourteen days after injection, the sciatic nerves were harvested and total oxidant status, total anti-oxidant status, paraoxonase-1, galectin-3 and matrix metalloproteinase 2 and 9 levels were measured in the sciatic nerves. In addition, the presence and status of inflammation, edema, and mast cells were evaluated histopathologically. Results: The combination of dexmedetomidine and bupivacaine alleviated oxidative stress. In addition, it decreased matrix metalloproteinase 9 and galectin-3 levels and increased matrix metalloproteinase 2 levels. Moreover, it stabilized recruited mast cells at the injury site; however, it did not significantly decrease inflammation or edema. Conclusion: Dexmedetomidine may ameliorate bupivacaine-induced neurotoxicity by modulating mast cell degranulation. The neuroprotective effect of dexmedetomidine may make it a suitable adjuvant agent to local anesthetics in peripheral nerve blocks.Öğe The protective effects of caffeic acid phenethyl ester in isoniazid and ethambutol-induced ocular toxicity of rats(Informa Healthcare, 2013) Sahin, Alparslan; Cingu, Abdullah Kursat; Kaya, Savas; Turkcu, Gul; Ari, Seyhmus; Evliyaoglu, Osman; Cinar, YasinPurpose: This study intended to examine the effect of caffeic acid phenethyl ester (CAPE) on isoniazid (INH) and/or ethambutol (ETM)-induced retina and optic nerve toxicity in a rat model. Methods: This study included eight groups, each containing 10 rats. The groups were Control, INH, ETM, CAPE, INH+CAPE, ETM+CAPE, INH+ETM and INH+ETM+CAPE. Rats were given orally 50 mg/kg/d of INH and 50 mg/kg/d of ETM in tap water for 30 d. 10 mu mol/kg of CAPE were intraperitoneally injected for 30 d. The first dose of CAPE was given 24 h before the INH and ETM treatment and continued until sacrifice. Control group was given only tap water for 30 d. Rats were anaesthetized and sacrificed on the 30th day of experiment. Superoxide dismutase (SOD) activities, malondialdehyde (MDA), total anti-oxidant status (TAS), total oxidant status (TOS) were measured on the dissected and excised retina and optic nerve samples. Fellow eyes were used for histopathologic evaluation and the retinal ganglion cell (RGC) count. In addition, CAPE, INH and ETM interaction with SOD isoforms were calculated in silico. Results: The SOD activity and TAS levels were found significantly higher in CAPE-treated groups compared to INH and/or ETM-treated groups (p<0.0001). But the MDA, and TOS levels were significantly lower in CAPE-treated groups (p<0.0001). The mean RGC count is significantly decreased in INH, ETM and INH+ETM groups compared with INH+CAPE, ETM+CAPE and INH+ETM+CAPE groups, respectively (p values 0.001, 0.042, and 0.001 respectively). Besides, in silico calculations showed that binding affinity of CAPE to SOD isotypes was higher than that of INH and ETM. Conclusion: This study demonstrates that CAPE treatment may decrease the oxidative stress in the retina and optic nerve of INH- and ETM-treated rats and may prevent RGC loss. As an underlying mechanism, CAPE and SOD interaction seems crucial for alleviation of ocular oxidative stress and RGCs toxicity.Öğe Serum IL-4 and IL-10 Levels Correlate with the Symptoms of the Drug-Naive Adolescents with First Episode, Early Onset Schizophrenia(Mary Ann Liebert, Inc, 2016) Simsek, Seref; Yildirim, Veli; Cim, Abdullah; Kaya, SavasObjective: In this study, it was aimed to understand the underlying possible immunopathogenesis of first episode, early onset schizophrenia (EOS) through profiling the T helper 1 (Th-1) cell cytokines TNF-, IFN-, and IL-2, Th-2 cell cytokines IL-4 and IL-10, Th-17 cell cytokine IL-17A, and inflammatory cytokine IL-6. Methods: The study included a total of 30 children, admitted to child psychiatry outpatient clinic aged between 10 and 17 years of age, who had not received prior therapy and were diagnosed with psychosis according to the Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV) as the patient group, and 26 age- and gender-matched children as the control group. Structured psychiatric interviews (K-SADS-PL and PANSS) were conducted with all participants. The BD Cytokine Bead Array Human Th-1/Th-2/Th-17 Cytokine Kit is used for the measurement of serum cytokines, for example, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-, and IFN-. Results: There was no significant difference between groups in terms of IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-, and IFN- levels (p>0.05). However, there was a significant correlation between IL-10 and IL-4 with negative symptoms of EOS (r=-0.65, p=0.02 and r=0.67, p=0.02, respectively). Conclusion: IL4 and IL-10 levels have a relationship with negative symptoms of disease. Therefore, this study might suggest that immunological processes might have a role in the disease pathophysiology.Öğe Serum omentin, resistin and tumour necrosis factor-? levels in Behcet patients with and without ocular involvement(Springer, 2015) Turkcu, Fatih Mehmet; Sahin, Alparslan; Cingu, Abdullah Kursat; Kaya, Savas; Yuksel, Hatice; Cinar, Yasin; Batmaz, IbrahimThe objective of this study was to evaluate serum omentin, resistin and tumour necrosis factor-alpha (TNF-alpha) levels in Behcet disease (BD) patients with and without ocular involvement, as well as control subjects. Omentin, resistin and TNF-alpha levels were assessed in the plasma of 51 BD patients and compared with those of 24 control subjects. The plasma resistin and TNF-alpha levels were significantly higher in the BD patients than in the control group (p = 0.028 and p = 0.011, respectively), whereas the plasma omentin level was significantly lower in BD patients than in the control group (p = 0.035). In the ocular BD, non-ocular BD and control groups, the omentin levels were 8.9 +/- 4.65, 8.6 +/- 3.61, and 12.4 +/- 6.24 ng/mL; resistin levels were 0.29 +/- 0.21, 0.24 +/- 0.2 and 0.15 +/- 0.45 ng/mL; and TNF-alpha levels were 25.45 +/- 3.65, 24.03 +/- 2.49 and 21.93 +/- 4.86 ng/mL, respectively. Omentin/resistin and TNF-alpha/omentin ratios were more significant parameters in the demonstration of the differences in the groups; the former was lower and the latter was higher in the patient groups (p = 0.001 and p = 0.002, respectively). We demonstrated that the plasma omentin level and omentin/resistin ratio were decreased, whereas the resistin and TNF-alpha levels and TNF-alpha/omentin ratio were increased in BD patients. These ratios may be used in the presentation of deviation in the inflammatory and anti-inflammatory balance in BD.Öğe Synthesis, characterization, DNA interaction and docking studies of novel Schiff base ligand derived from 2,6-diaminopyridine and its complexes(Elsevier, 2020) Kurt, Baris; Temel, Hamdi; Atlan, Metin; Kaya, SavasIn this study [1-(4-{[(3-hydroxynaphthalen-2-yl)methylidene]amino}phenyl)ethan-1- one (B-1 ligand) were synthesized from the reaction of 2-hydroxy-1-naphthaldehyde with 4-amino-acetophenone. Following that 1-(N-{4-[(1)-1-({6-[(Z)-[1-(4-{[(3- hydroxynaphthalen-2-yl) methylidene]amino}phenyl) ethylidene]amino]pyridine2- yl}imino)ethyl]phenyl}carboximidoyl)naphthalen-2-ol (L-1 ligand) were synthesized from the reaction of 2,6-diamino pyridine with B-1 ligand. Using this Schiff base ligand Cu(II), Fe(II) and Pd(II) complexes were prepared. The structure of the ligand and its complexes were determined by H-1 NMR, FTIR, UV-Vis, elemental analysis, thermal analyses and magnetic susceptibility spectral data. Further, DNA binding properties of the ligand and its metal complexes were investigated and docking studies were carried out. As a result ligands and copper complex showed DNA binding activity and an increase with H2O2. According to these results it can be suggested that these compounds are very suitable drug candidates. (C) 2020 Elsevier B.V. All rights reserved.
