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The accumulation and histological effects of organometallic fungicides propineb and maneb in the livers of pregnant rats and their offspring
(Japanese Society of Toxicology, 1999) Deveci, Engin; Güven, Kemal; Başhan, Mehmet; Önen, Abdurrahman; De Pomerai, David
Dithiocarbamate propineb and maneb are organometal fungicides, which are widely used for the control of diseases in plants. Female Wistar rats were exposed orally to 200 and 400 ppm propineb (Zn-containing dithiocarbamate) and 250 ppm maneb (Mn-containing dithiocarbamate), from the 6th day of gestation up to birth. We found that the body weights of both newborn litters and their fungicide-treated mothers were lower than those of controls. Histological examination of the livers of fungicide-treated pregnant females and the offspring showed a variety of histopathological effects. Moreover, the analysis of Zn and Mn concentrations in the livers of pregnant females exposed to organometallic fungicides during pregnancy demonstrated that the metal concentrations in the liver were higher than those of controls. Similarly, the hepatic metal concentrations were significantly increased in the litters, indicating the transplacental passage of the organometallic fungicides.
The accumulation and histological effects of the organometallic fungicide propineb on the organs of fetuses and female rats during pregnancy
(1999) Pomeraı, David De; Güven, Kemal; Deveci, Engin
Ditiyokarbamat yapısında olan propineb bitkisel hastalıkların kontrolünde yaygın bir şekilde kullanılan bir organometal fungisittir. Hamileliğin 6. gününden doğuma kadar 200 ve 400 ppm'lik propineb konsantrasyonları dişi VVİstar ratlara ağız yolu ile verilmiş; 400 ppm'lik konsantrasyonda hem fetüs hem de dişi ratlann organlarında bir çok makroskopik (ör. pitosis ve felç) ve mikroskopik septomlara rastlanmıştır. Yapılan çalışmada, fungisit ile muamele edilen anne ve yavrularının vücut ağırlıkları kontrolden daha düşük bulunmuştur. Fetüs ve propineb verilen annenin karaciğer ve böbreklerinde yapılan histolojik inceleme sonucunda çok sayıda histopatolojik etkiye rastlandı. Hamilelik esnasında organometal fungisit verilen hamile dişilerin karaciğer, böbrek ve kalplerindeki Zn konsontrasyon analizi (ICP-AES;İnductively Coupled Plasma-Atomic Emission Spectrometer The Accumulation and I listological Effects of The Organometallic Fungicide Propineb on The Organs of Fetuses and Female Rats During Pregnancy kullanarak) bu organlarda metal konsantrasyonlarının kontrole nazaran oldukça yüksek olduğu bulundu. Bununla beraber, hamilelik esnasında fungisite maruz kalan yavruların karaciğer, böbrek ve kalplerindeki Zn konsantrasyonları kontrole nazaran çok büyük oranlarda artmıştır; fetüsün organlarında bulunan bu yüksek metal seviyeleri fungusitin plasental engeli rahatlıkla geçebildiğinin bir göstergesi olabilir.
ACUTE AND LONG-TERM PATHOLOGICAL EFFECTS OF COVID-19 ON THE PLACENTA DURING SECOND TRIMESTER AND LABOR
(Univ Quindio, 2023) Tast, Fatih; Erdemci, Fikri; Yilmaz, Mehmet; Asir, Firat; Ozudogru, Osman; Deveci, Engin
Background: COVID-19 pandemic has affected all the world. The consequence of the COVID-19 infection causes many disorders in many organs, one of them is the placenta. COVID-19 disease has long-term effects on various tissues after recovery. The aim of this study was to investigate the placentas of pregnant women with healthy, COVID-19 positive during the second trimester and labor. Material and methods: A total of twenty-four pregnant women (8 patients per each group) were included in the study. Their placentas were processed for routine paraffin wax embedding protocol. The blood parameters of patients were recorded. Placental tissues were stained with hematoxylin-eosin dye and immune stained with TNF-a and ADAMTS-8. Statistical analysis was performed on blood and histological parameters.Results: AST and CRP values of biochemical parameters were higher in women with second trimester and labor groups than in the healthy group. Also, a significant increase in ALT values was observed in the labor group. Normal histology was observed in the placentas of healthy patients. More histopathology was recorded in the placentas of COVID-19 infected women compared to healthy placentas. The expression of TNF-a and ADAMTS-8 were found significantly higher in the COVID-19 placentas compared to the non-COVID-19 group.Conclusions: COVID-19 infection can cause pathological changes during pregnancy and labor. This study shows that COVID-19 not only acutely has adverse effects on placental pathologies but also has long-term effects. TNF-a and ADAMTS-8 primary antibodies can be a guide in demonstrating these effects.
ADM and SFLT-1 Eexpression in placentas with gestational diabetes mellitus
(Merthan Tunay, 2023) Arslan, Necat; Söker, Sevda İpek; Başaran, Süreyya Özdemir; Kaplan, Özge; Aşır, Fırat; Deveci, Engin; Şeker, Uğur
Aim: In this study, we aimed to examine the expression levels of Adrenomedullin (ADM) and soluble fms-like tyrosine kinase-1 (sFlt-1), two novel proteins found to be involved in vascular regulation in gestational diabetes mellitus (GDM), to compare the expression levels of these proteins in the histopathology of the disease and to observe the correlation of the expression intensity of these proteins with the disease. Methods: In our study, 20 healthy and 20 GDM placenta samples were obtained. Histologic followup was performed. 5µm thick sections were taken from these tissues and stained with HematoxylinEosin and Periodic Acid Schiff (PAS). Immunohistochemically, ADM and sFlt-1 antibodies were studied. Results: In the GDM group, vascular dilatation and congestion in stem villus, hyperplasic endothelial cells, and increased syncytial bridges in the external part of the villi, mononuclear cell infiltration, pyknotic nuclei and cytoplasm loss in some of the decidual cells in the maternal region were observed. In the immunohistochemical examination, cytotrophoblast and syncytiotrophoblast cells of villous and syncytial nodes showed negative ADM expression. ADM was positively expressed in some cytotrophoblast cells of small villi, vascular endothelial cells and decidual cells. In the GDM group, sFlt-1 expression was positive in endothelial cells, some Hofbauer cells of mesenchymal connective tissue, decidual cell nuclei and membranes. Conclusions: ADM may be an important receptor in insulin metabolism to determine the glucose level because we found positive ADM expression in cytotrophoblasts and membranes of decidual cells. In addition, changes in endothelial cells of maternal and fetal regions and sFlt-1 expression in Hofbauer cells suggest that this molecule possibly plays a key role in the angiogenic effect.
Alloplastik kemik grefti uygulanmış sıçan kalvarial kemik defekt modelinde rosmarinik asidin terapötik etkileri
(Mersin University, 2022) Deveci, Busra; Dağ, Ahmet; Kadiroğlu, Ela Tules; Aşır, Fırat; Gokalp-ozkorkmaz, Ebru; Deveci, Engin
Amaç: Travma, neoplazmalar, enfeksiyonlar ve doğuştan anomaliler gibi pek çok neden, kalvaryal kemik kusurlarına neden olabilir. Kraniyal bölgedeki kemik kusurlarını gidermek ve kemik yenilenmesini uyarmak için çeşitli greft tipleri denenmiş ve tedavi yöntemleri araştırılmıştır. Çalışmamızda Rosmarinik asidin (RA) greft materyali uygulanmış sıçan kalvaryal kemik defekt modeli üzerindeki etkilerinin araştırılması amaçlanmıştır. Yöntem: Çalışmada Wistar erkek sıçanlar (n=32) dörde bölündü; kontrol olarak defekt grubu (n=8), defekt+RA uygulamalı grup (n=8), defekt+greft (n=8), defekt+greft+RA uygulamalı grup (n=8). Anestezi altında frontal kemik açılarak ve periosteal elevatör ile periosteal flep çıkarıldı ve orta hatta dairesel tam kalınlıkta kemik defekti (5 mm) oluşturuldu. Grup iki ve üçte defekt bölgesine allogreft materyal yerleştirildi, doku dikilerek kapatıldı. RA (100mg/kg) oral yolla 7 gün süre ile uygulandı. 28 gün sonra tüm hayvanlar sakrifiye edildi ve kalvaryal kemikler çıkarıldı, %10’luk formalin ile tespit edildikten sonra, %5’lik EDTA (Etilendiamin tetraasetik asit) ile dekalsifiye edildi, rutin histolojik preparasyondan sonra 4-6 µm kalınlığında kesitler kesildi ve Hematoksilen Eosin ile boyandı. BMP-2 (Bone morphogenetic proteins) kemik morfogenetik proteinleri immunohistokimyasal olarak işaretlendi ve ışık mikroskobu altında incelendi. Kan örneklerinden alkalen fosfataz (ALP), kreatin kinaz (KK), total antioksidan seviyesi (TAS), total oksidan seviyesi (TOS) ve oksidatif stres indeksi (OSI) parametreleri çalışıldı. Bulgular: TAS değeri defekt+greft+RA grubunda defekt grubuna göre anlamlı olarak yüksek bulunmuştur (p<0.05). OSI değeri defekt+greft+RA grubunda defekt grubuna göre anlamlı olarak düşüktür. Greft alanı içinde inflamatuvar hücrelerin az sayıda soliter olarak dağıldığı, kan damarlarında dilatasyon ve konjesyonun azaldığı tespit edildi. Ana kemik bölgesine yakın kemik tarbeküllerin genişlediği osteoblastik aktivitenin belirginleştiği matriks yapısının olgunlaştığı ve osteositlerin laküna içinde yerleştiği görüldü. Sonuç: Sonuç olarak, RA+Greft uygulaması ile kalvariyal defekt modelinde osteoblastik aktiviteyi indükleyerek osteosit oluşumuna ve yeni kemik gelişimine neden olduğu gözlenmiştir.
Antiglycative effect of combination of extracts of capsicum annuum (chilli) and pyridoxamine against glycation in streptozotocin-induced experimental diabetes in rats
(Springer Science and Business Media, 2023) İnceören, Nesrin; Akay, Feryal; Nas, Cemal; Deveci, Engin; Kızıl, Göksel; Kızıl, Murat
Glycation causes the formation of advanced glycation end products, which play an important role in the pathogenesis of diabetic complications, which alter changes the structure and functional properties of proteins that negatively afect cellular metabolism. In the current study, we investigated benefcial efects of red chili pepper extract and capsaicin, the main bitter ingredient in red peppers and pyridoxamine, a vitamin B6, one by one or in combination on glycation and advanced glycation end products damage in streptozotocin-induced diabetic rats. The total amount of capcaicin in red chili pepper extract was found to be 3.51 mg/g DW by HPLC. Male Sprague Dawley rats (8 weeks old), weighing 200–300 g, were divided into 12 groups of 8 rats each. A single dose of streptozotocin (50 mg/kg body weight) was administered to induce experimental diabetes in rats. Group 1 was normal control, group 2 was diabetic control. Groups 3 and 4, 200 and 400 mg/kg red chili pepper extract, groups 5 and 6, 6 and 8 mg/kg capsaicin and group 7, 4 mg/ml pyridoxamine and group 8 were receiving 300 mg/kg metformin. Groups 9 and 10, 200 and 400 mg/kg red chili pepper extract+4 mg/ml PM and groups 11 and 12 were diabetic rats treated with 6 and 8 mg/kg capsaicin+4 mg/ml pyridoxamine. Oral treatment with intragastric tube was administered every day for 28 days. At the end of the experiment, subjects in all groups were given anesthesia once and the rib cage was performed by intracardiac intervention and blood was collected. Immediately, the liver and kidneys were grossly removed for histological examination. The results of our study showed that the combined doses of red chili pepper extract+pyridoxamine and capsaicin+pyridoxamine signifcantly reduced blood glucose levels, serum uric acid, urea, creatinine, AST, ALT, ALP, LDH, total cholesterol, fructosamine, HbA1c levels and signifcantly increased serum HDL and insulin levels. At the same time, histopathological studies also strongly supported the anti-diabetic, hepatoprotective and renoprotective efects of these combination doses.
Biochemical and Histopathological Investigation of Resveratrol, Gliclazide, and Losartan Protective Effects on Renal Damage in a Diabetic Rat Model
(Sci Printers & Publ Inc, 2015) Ezel, Tasdemir; Kocyigit, Yuksel; Deveci, Engin; Atamer, Yildiz; Sermet, Abdurrahman; Uysal, Ersin; Aktas, Ayfer
[Abstract Not Available]
Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue
(Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia, 2023) Toprak, Veysel; Akalın, Senem Alkan; Öcal, Ece; Çavuş, Yunus; Deveci, Engin
Purpose: It was aimed to investigate the biochemical and immunohistochemical effects of ephedrine (EPH) in bilateral ovariectomized rats. Methods: Twenty-four Sprague Dawley female rats were divided into three groups: control group: The abdomen was opened and closed without any treatment; ischemia-reperfusion (IR) group: 2 h of ischemia followed by 2 h of reperfusion were allowed to cause IR injury; IR+EPH group: oral EPH solution (5 mg/kg) was administered for 28 days. Results: Biochemical parameters were statistically significant in group comparisons. Increased interleukin-6 (IL-6) expression, degenerative preantral and antral follicle cells and inflammatory cells around blood vessels were seen in IR group. Negative IL-6 expression was observed in seminal epithelial cells, preantral and antral follicle cells in IR+EPH group. While caspase-3 activity increased in granulosa cells and stromal cells in IR group, caspase-3 expression was negative in preantral and antral follicle cells in the germinal epithelium and cortex in IR+EPH group. Conclusion: The effect of apoptosis, which occurs with the signaling that starts in the cell nucleus, caused the cessation of the stimulating effect at the nuclear level after EPH administration, and a decrease in the antioxidative effect in IR damage and inflammation in the apoptotic process.
The changes in Hoffbauer and syncytiotrophoblast cells in serious preeclampsia complicated with hellp syndrome (Ultrastructural and immunohistochemical study)
(INESEG Yayıncılık, 2022) Nergiz, Yusuf; Nergiz, Şebnem; Aşır, Fırat; Deveci, Engin; Sak, Erdal; Evsen, Sıddık; Tunik, Selçuk; Şeker, Uğur
HELLP syndrome is a syndrome characterized by hemolytic anemia, increased liver enzymes, and thrombopenia and can be seen in 1% of all pregnancies, 10-20% of pregnancies with pain, preeclampsia, and eclampsia. HELLP syndrome usually develops in the third trimester and its pathogenesis is not clear. Human placental villus stroma contains macrophages called Hoffbauer cells (HC), which are thought to be involved in many processes. HC is also called placental macrophage and has a role in many placental events. This study aimed to evaluate the immunohistochemistry and ultrastructural of syncytiotrophoblast and Hoffbauer cells in the placental villi of HELLP syndrome patients. In our study, placental tissues obtained from human normal and HELLP syndrome pregnancies were prepared for light and transmission electron microscopy (TEM) studies. Immunohistochemistry techniques were applied to placenta sections. HC localizations were determined with CD68 (Hoffbauer cell marker). Fine structure properties of HC and syncytiotrophoblasts were examined by TEM. When the HELLP group fetal placental sections were examined under the light microscope, intracytoplasmic edema in syncytiotrophoblast, degenerative vacuoles, and degenerative findings on cell surface membranes were observed. Moreover, villous edema was remarkable. The increase in the number of Hoffbauer cells per villus in the HELLP group was statistically significant (p<0.00). Compared with the control group, there was a significant increase in the number of Hoffbauer cells and syncytiotrophoblast in HELLP group, and also degenerative changes were observed in the ultrastructural structure of these cells.
Changes in the Bladder After Spinal Cord Injury and Expression of VEGF and APAF-1
(Sci Printers & Publ Inc, 2021) Yamis, Mehmet; Deveci, Engin
OBJECTIVE: A spinal cord injury (SCI) is damage to the spinal cord either from trauma, loss of its normal blood supply, or compression from tumor or infection. In this study we focused on alterations in the bladder tissue with angiogenic and apoptotic aspects after spinal cord injury. STUDY DESIGN: Twenty Wistar Albino rats were categorized as control and SCI groups. At T7-T9 vertebras, a steel rod was dropped from 10 cm to create a spinal cord injury under anesthesia. Rats were decapitated and spinal tissue was processed to measure malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO). RESULTS: MDA, MPO, epithelial degeneration, vascular dilation, inflammation, VEGF, and APAF-1 expressions in the SCI group were statistically higher than those in the control group. GSH content of the SCI group was statistically lower than that in the control group. In the hematoxylin-eosin-stained sections of the control group, normal histology was observed in bladder tissue. In the SCI group, degeneration epithelial cells, thinned epithelium, increased fibrosis, dilated and congested blood vessels, and hyperplastic endothelial cells were observed. In the control group, VEGF expression was slightly observed in some epithelial cells and vascular cells. In the SCI group, VEGF expression was increased in inflammatory and vascular endothelial cells. For APAF-1 expression, the control group showed no expression. In the SCI group, APAF-1 expression was positive in degenerated epithelial cells and connective tissue cells. CONCLUSION: It is thought that the urination reflex was affected due to increased inflammation in the bladder tissue, leading to alterations in the regulation and function of the muscles.
Comparison of histopathologic findings of initial and recurrent lumbar disc herniation
(Taylor & Francis Ltd, 2024) Ozevren, Huseyin; Cetin, Abdurrahman; Baloglu, Murat; Deveci, Engin
ObjectiveRecurrent lumbar disc hernia (RLDH) is a common and challenging complication after an initial discectomy. This study aimed to investigate the relationship between the histopathologic outcomes of the initial and recurrent disc tissues.MethodsThis study investigated 70 patients who underwent a microdiscectomy and subsequently developed same-level same-side lumbar disc herniation (LDH) recurrence. The clinic, western blot, and immunohistochemical evaluations of patients with initial LDH and RLDH were conducted and statistically analyzed.ResultsThe effect of inflammation and apoptosis in the degenerative changes of intervertebral disc hernia and increased histopathologic findings in RLDH was demonstrated. The degeneration of the hernia disc tissue is a major pathological process, which is characterized by cellular apoptosis, inflammation, and reduced synthesis of extracellular matrix. Currently, there is no clinical therapy targeting the reversal of disc degeneration.ConclusionsThis, therefore, stay away from factors that increase inflammation in the intervention of intervertebral disc hernia, applying to reduce inflammation the medicines, could allow reducing disc collagen degeneration, and more successful outcomes. These findings might shed some new lights on the mechanism of disc degeneration and provide new strategies for the treatments of initial and recurrent LDH.
Conus Medullaris Position in an Adult Population: Analysis of Magnetic Resonance Imaging
(Soc Chilena Anatomia, 2016) Karabulut, Özlen; Akay, Hatice; Karabulut, Zülfü; Özevren, Hüseyin; Saka, Günay; Hatipoğlu, Savaş; Deveci, Engin
The objective of this study was to evaluate the variation in position of the conus medullaris (CM) in male and female patients without spinal deformity, to correlate the termination level in magnetic resonance (MR) images of the lumbar spine. 921 patients consisted of 607 men and 314 women were evaluated by MRI. The strength of T1 weighted MRI device was 1.5 Tesla. The patients were in supine position when measured. The termination level of the conus medullaris was recorded in relation to the upper, middle or lower third of the adjacent vertebra and the adjacent intervertebral disc. The patients in our study group were examined for low back pain. The members with spinal deformity were excluded. The distribution of conus medullaris localization was measured to range from T12 to L2-L3. There was a statistically significant difference in the mean conus medullaris position related to gender also a significant difference between increasing age and conus position in female patients. These findings suggest that the distribution of CM location in a large adult population was shown to range from the upper third of T12 to the lower third of L2-L3 disc space both in women and men.
Dalak tüberkülozlu hastada splenektomi: Olgu sunumu
(2004) Önen, Abdurrahman; Yaramış, Ahmet; Şenyiğit, Abdurrahman; Deveci, Engin
Yaklaşık 20 aylık halsizlik, iştahsızlık, öksürük, ateş, gece terlemesi ve kilo kaybı yakınmaları olan 4 yaşında erkek çocuk hastanemize getirilmiş, bir yıldan beri akciğer tüberkülozu tanısıyla antitüberküloz tedavi almasına karşın genel durumunda anlamlı düzelme olmadığı öğrenilmiştir. Ön-arka akciğer filiminde mikronodüler infiltrasyon, karın ultrasonografisi ve bilgisayarlı tomografisinde dalakta çok sayıda nodül ve/veya mikroapseler saptanmış ve splenektomi yapılmıştır. Ameliyatta dalak tamamen kalsifiye çok sayıda nodül ve mikroapselerle kaplanmış olarak bulunmuştur. Splenektomi sonrası, erken dönemde genel durumu düzelen ve yakınmaları gerileyen hastaya iki yıl boyunca antitüberküloz tedavi verilmiştir. Dört yıllık izlem sonrası son kontrolünde yakınması olmayıp genel durumu iyi ve gelişimi normaldir. Dalak tüberkülozu sıklığı son zamanlarda artmıştır. Özellikle tüberkülozun sık gö-rüldüğü bölgelerde tüberkülöz dalak apselerinden şüphenilmelidir. Erken tanı ve erken ilaç tedavisi önemlidir. Erken tedavi edilmeyen veya tedaviye dirençli dalak abseli olgular splenektomiden yarar görebilir.
Deneysel Tavmatik Omurilik Hasarında Thymbra Spicata L. var Spicata ve Cyclotrichium Origanifolium’un Mesane Üzerine Koruyucu Etkisinin Araştırılması
(Akdeniz Üniversitesi, 2025) Direk, Figen Koç; Durmaz, Sevda Canbay; Tuncer, M. Cudi; Karabaş, Sibel Ateşoğlu; Keşim, Dilek Aygün; Nas, Cemal; Deveci, Engin
ÖZ Amaç: Spinal kord yaralanmaları, genellikle travma sonrası vertebral kolonda meydana gelen kırıklar, çıkıklar, bağ yaralanmaları ve yırtılmalar sonucunda oluşan hasarları kapsamaktadır. Bu yaralanmalarda komplikasyonların en sık görüldüğü organlardan biri mesanedir. Thymbra spicata L. var spicata (zahter) ve Cyclotrichium origanifolium (dağ nanesi) bitkilerinin yüksek biyolojik potansiyele sahip olduğu; ekstraktlarının antioksidan özellikleri sayesinde oksidatif stresin neden olduğu hastalık riskini azalttığı ve tedavide önemli bir rol oynadığı bilinmektedir. Bu çalışmada, zahter ve dağ nanesi ekstraktlarının spinal kord yaralanması sonrası mesane disfonksiyonları üzerindeki potansiyel tedavi edici ve koruyucu etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Çalışmada, ağırlıkları 250-300 g arasında değişen toplam 36 adet Wistar Albino erkek sıçan, her grupta altı sıçan olacak şekilde altı gruba ayrılmıştır: Grup 1 (kontrol), Grup 2 (zahter), Grup 3 (dağ nanesi), Grup 4 (travma), Grup 5 (travma + zahter), Grup 6 (travma + dağ nanesi). Travma gruplarında omuriliğe ağırlık düşürme yöntemi uygulanarak travma modeli oluşturulmuştur. Bir haftasının sonunda, tüm sıçanlardan idrar örnekleri alındıktan sonra, ketamin anestezisi altında sakrifiye edilerek mesane dokuları alınmıştır. Mesane dokularında histopatolojik değişiklikler incelenmiştir. Bulgular: Deneysel araştırma sonucunda, zahter ve dağ nanesi ekstraktlarının spinal kord yaralanmasına bağlı gelişen mesane hasarına karşı kısmen etkili olduğu saptanmıştır. Zahterin etkinliğinin, biyokimyasal ve histopatolojik bulgular doğrultusunda, oksidatif stres ve inflamatuar belirteçler üzerindeki etkisinin dağ nanesine kıyasla daha yüksek olduğu gözlemlenmiştir. Sonuç: Sonuç olarak, deneysel modellerin artmasıyla birlikte bitki ekstraktlarının tedavi amaçlı kullanımının yaygınlaşabileceği ve insan sağlığı üzerinde olumlu etkiler sağlayabileceği düşünülmektedir.
Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells
(Dicle Üniversitesi Tıp Fakültesi, 2018) Yavaş, Mehmet Cihan; Akpolat, Veysi; Deveci, Engin; Bilgin, Hakkı Murat; Kaplan, İbrahim; Şeker, Uğur; Yıldız, İsmail; Alkış, Mehmet Eşref; Çelik, Mustafa Salih; Akdağ, Mehmet Zülküf
Abstract:Objective: Purpose of the study was to research the effect of an electromagnetic field created by a high voltage line onthe testes tissue and the serum biochemistry of Wistar albino male rats. At the same time, in the study also examinedthe protective effects of melatonin and ganoderma lucidum.Methods: In the study, 64 rats were divided into eight equal groups (n: 8). In experiments lasting 26 and 52 days, thefollowing groups were used: High voltage (HV), HV + Ganoderma lucidum (GI), HV + Melatonin (MEL), and Shamcontrol.MEL (10 mg/kg) was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely lowfrequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied for eight hours per day. Results: The biochemical results of the study show an increase in total oxidant status (TOS) and oxidative stress index(OSI) level and a decrease in total antioxidant status (TAS) level in the 26 and 52 day high voltage groups, comparedto the control group. In the high voltage groups, the nitric oxide (NO) value increased with the increase of theexposure period. Testicular mass (p>0.05), TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison.Testicular weight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 day comparisons. In routinehistopathology data, we detected effects on both the 26 and 52 day HV groups, such as degeneration in spermatic cellsand full structural deterioration and increasing hyalinisation. We also detected an step up in the ligament cell in thetubular field for the 52-day test groups. In melatonin added group, proliferating cell nuclear antigen (PCNA)-positivecells were more active. TUNEL assay analysis showed that there was no significant difference between the the 26 dayexperiment groups (p>0.05), but that there was a significant dissimilarity between the 52 day experiment groups(p<0.05).Conclusions: In our study, the oxidative effect of created by a high voltage results in changes in serum biochemistry ofrats and a number of degenerative deterioration in the histological structure of testes. Aslo, it is shown that theoxidative effect created by a high voltage has negative effects on the creation of spermatogonia. GI partly protectsagainst these effects, while melatonin is more effective in this regard.
Diyabetik sıçanların testis dokusunda VEGF ve Bcl-2 ekspresyon düzeylerinin immünohistokimya ve Western Blot yöntemleri ile incelenmesi
(Dicle Üniversitesi Tıp Fakültesi, 2016) İrtegün, Sevgi; Deveci, Engin
Amaç: Bu çalışmada diyabetik sıçanların tetis dokusunda anjiogenezi ve vasküler geçirgenliği stimule eden vasküler endotel büyüme faktörünün (VEGF) ve apoptozis regülasyonunda önemli bir rol oynayan B-cell lymphoma-2’ nin (Bcl-2) ekspresyon düzeylerini araştırmayı amaçladık. Yöntemler: Bu çalışmada 20 adet erişkin Wistar albino sıçan kullanıldı. Sıçanlar kontrol ve Diabetes mellitus (DM) olmak üzere 2 eşit gruba ayrıldı. DM oluşturmak için sitrat tamponunda çözülmüş tek doz Streptozotosin (STZ) (55mg/kg) intraperitoneal olarak verildi. %10’ luk formaldehit solüsyonuna atılan testis dokuları rutin parafin takiplerinden sonra histopatolojik olarak incelendi. VEGF ve Bcl-2 protein düzeyleri immünohistokimya ve Western Blot yöntemleriyle ölçüldü. Bulgular: Diyabetik gruba ait spermatik hücrelerin bazılarında dejenerasyon, çekirdekte küçülme ve yer yer piknosis gözlendi. Diyabet sonucu Sertoli hücrelerinde yapısal değişiklikler saptanırken, tubuller arasındaki damarlarda dilatasyon ve hemoraji gözlendi. Diyabetin etkisi ile testis dokusunda VEGF ekspresyonunun bloke olduğu, Bcl-2 ekspresyonunun ise azaldığı saptandı. Sonuç: Diyabetik testis dokularında VEGF ekspresyonunun bloke olmasının yetersiz anjiogenez ve vasküler permeabiliteye neden olabileceği olasıdır. Ayrıca diyabetik testis dokularında anti-apoptotik protein olan Bcl-2 ekspresyonundaki azalmanın diyabetin testiküler dokuda apotozis regülasyonunun bozulmasına neden olabileceği düşünülmektedir.
Diyabetik Sıçanların Testis Dokusunda VEGF ve Bcl-2 Ekspresyon Düzeylerinin İmmünohistokimya ve Western Blot Yöntemleri İle İncelenmesi
(2016) Deveci, Engin; Kandemir, Sevgi İrtegün
Amaç: Bu çalışmada diyabetik sıçanların tetis dokusunda anjiogenezi ve vasküler geçirgenliği stimule eden vasküler endotel büyüme faktörünün (VEGF) ve apoptozis regülasyonunda önemli bir rol oynayan B-cell lymphoma-2' nin (Bcl-2) ekspresyon düzeylerini araştırmayı amaçladık. Yöntemler: Bu çalışmada 20 adet erişkin Wistar albino sıçan kullanıldı. Sıçanlar kontrol ve Diabetes mellitus (DM) olmak üzere 2 eşit gruba ayrıldı. DM oluşturmak için sitrat tamponunda çözülmüş tek doz Streptozotosin (STZ) (55mg/kg) intraperitoneal olarak verildi. %10' luk formaldehit solüsyonuna atılan testis dokuları rutin parafin takiplerinden sonra histopatolojik olarak incelendi. VEGF ve Bcl-2 protein düzeyleri immünohistokimya ve Western Blot yöntemleriyle ölçüldü. Bulgular: Diyabetik gruba ait spermatik hücrelerin bazılarında dejenerasyon, çekirdekte küçülme ve yer yer piknosis gözlendi. Diyabet sonucu Sertoli hücrelerinde yapısal değişiklikler saptanırken, tubuller arasındaki damarlarda dilatasyon ve hemoraji gözlendi. Diyabetin etkisi ile testis dokusunda VEGF ekspresyonunun bloke olduğu, Bcl-2 ekspresyonunun ise azaldığı saptandı. Sonuç: Diyabetik testis dokularında VEGF ekspresyonunun bloke olmasının yetersiz anjiogenez ve vasküler permeabiliteye neden olabileceği olasıdır. Ayrıca diyabetik testis dokularında anti-apoptotik protein olan Bcl-2 ekspresyonundaki azalmanın diyabetin testiküler dokuda apotozis regülasyonunun bozulmasına neden olabileceği düşünülmektedir.
Doğumdan sonra rat penisinin gelişimi üzerine tamoxifen'nin etkisi
(2015) Deveci, Engin
Gebe raflardan doğan erkek yavrulara, doğumun sıfırına gününden beşinci gününe kadar günlük 100 ug Tx subkutan olarak enjekte edildi. Total olarak alınan penisler %10 luk notral formalinde fikse edildekten sonra parafin inklüzyonuna alındı, elde edilen parafin kesitleri Hematoksilen-Eozin, Verhoeff ve Tripple ile boyandılar. Os penis ve glans penisin gelişimindeki yapısal değişiklikler incelendi. Ratlar 7,14,21,28,35 ve 60. günlerde sakrifiye edilip penisleri alındı. Çalışmamızda, kontrol grubu os penisin proksimal segmentinde hyalin kıkırdak, kemik iliği ve trabekül oluşumu doğumdan sonra 7. günde saptanırken; 28-60 günlük kontrol grubu ratlarda distal segmentte fibrokartilajinöz bir doku gözlendi. Tx enjekte edilen ratlarda dördüncü haftada hemopoetik doku, altmışıncı günde ise hyalin kıkırdağın ortadan kalktığı gözlendi. Tx verilen 28-60 günlük rat penislerinin distal segmentinde fibrokartilajinöz dokuya rastlanılmadı. Yirmibir günlükten itibaren Tx verilen hayvanların glans penisinde epidermal çıkıntıların yavaş yavaş silindiği, altmışıncı günde ise epidermal çıkıntı ve keratinleşmenin tamamen ortadan kalktığını izledik. Sonuç olarak neonatal dönemde Tx verilen ratların, os penisinde fibrokartilajinöz doku, hyalin kıkırdak ve glans penisteki epidermal çıkıntıların gelişiminin engellendiğini saptadık.
Effect of aloe vera on Mmp-1 and Timp-1 expression on diabetic wound healing
(Merthan Tunay, 2023) Seyrek, Rohlat; Söker, Sevda; Kaplan, Özge; Başaran, Süreyya Özdemir; Aşır, Fırat; Deveci, Engin; Şeker, Uğur
Aim: The aim of this study is to investigate the healing aspect of aloe vera in diabetes mellitus, which inhibits wound healing. Methods: Diabetes model was created with streptozotocin. At the end of the 14-day experiment, blood glucose was measured from the tail vein of animals in all groups and blood was taken from the heart and sacrificed. Histopathology and immunohistochemical statistics and evaluation were performed. Results: Pycnosis and degeneration of epithelial cells were observed in diabetes groups. Leukocyte infiltration in the dermal papilla, degeneration of collagen fibers and an increase in the extracellular matrix were observed. It was observed that the epithelial layer in the aloe vera group was histologically close to the control group. It was observed that decreased inflammation in the dermal papilla and decreased in organized collagen fibers and vessel dilatation were observed. In the control group, MMP-1 and TIMP-1 expression were positive in the epidermis and dermis layers. In the diabetes group, weak expression of MMP-1 and TIMP-1 was observed in cells in the epidermis and dermis. The expression of MMP-1 and TIMP-1 in the surface epithelium in the aloe vera group was increased compared to the diabetes group. Conclusions: Aloe vera accelerated cell and extracellular matrix regeneration with its anti-oxidative activity.
Effect of cadmium chloride toxicity on olfactory mucosa
(Universidad de la Frontera, 2022) Haznedar, Berzan; Gök, Ertuğrul; Deveci, Engin
Cadmium is a highly toxic metal and affects the respiratory mucosa. The aim of the study is to show the inflammation and degenerative effect of cadmium on the olfactory mucosa. In this study, eight-week-old Wistar rats with an average weight of 170-190 g were divided into two groups (control and experiment) with 20 animals in each group and used in the experiments.The rats in the experimental group were given 2 mg/kg/day powdered cadmium chloride dissolved in water intraperitoneally every day for two weeks. At the end of the experiment, the nasal cavity was completely removed with anesthesia. Concha nasalis superior was separated, fixed with zinc-Formalin solution and decalcified with 5 % EDTA (Ethylene-diaminetetraacetic acid).After routine histopathological procedure, APAF-1 antibody was used for expression of Hematoxylin-Eosin (HE) and immunohistochemistry. Histopathological examination revealed interruptions in the basement membrane structure due to cadmium and degenerative changes in stem cells, degeneration in sensory cells and pycnosis in nuclei, dilatation in blood vessels and increased inflammation in connective tissue. APAF-1 expression was found to increase in epithelial cells and olfactory glands (Bowman gland) cells. It has been thought that cadmium toxicity increases cell degeneration and inflammation in the olfactory mucosa and may significantly affect cell death and olfactory metabolism by inducing the pro-apoptotic process.

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