Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: It was aimed to investigate the biochemical and immunohistochemical effects of ephedrine (EPH) in bilateral ovariectomized rats. Methods: Twenty-four Sprague Dawley female rats were divided into three groups: control group: The abdomen was opened and closed without any treatment; ischemia-reperfusion (IR) group: 2 h of ischemia followed by 2 h of reperfusion were allowed to cause IR injury; IR+EPH group: oral EPH solution (5 mg/kg) was administered for 28 days. Results: Biochemical parameters were statistically significant in group comparisons. Increased interleukin-6 (IL-6) expression, degenerative preantral and antral follicle cells and inflammatory cells around blood vessels were seen in IR group. Negative IL-6 expression was observed in seminal epithelial cells, preantral and antral follicle cells in IR+EPH group. While caspase-3 activity increased in granulosa cells and stromal cells in IR group, caspase-3 expression was negative in preantral and antral follicle cells in the germinal epithelium and cortex in IR+EPH group. Conclusion: The effect of apoptosis, which occurs with the signaling that starts in the cell nucleus, caused the cessation of the stimulating effect at the nuclear level after EPH administration, and a decrease in the antioxidative effect in IR damage and inflammation in the apoptotic process.
Açıklama
Anahtar Kelimeler
Antioxidants, Apoptosis, Caspase 3, Interleukin-6
Kaynak
Acta Cirurgica Brasileira
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
38
Sayı
Künye
Toprak, V., Akalın, S. A., Öcal, E., Çavuş, Y. ve Deveci, E. (2023). Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue. Acta Cirurgica Brasileira, 38, 1-8.
