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Öğe Atorvastatinin sıçan iskelet kasında oluşturduğu yapısal değişikliklerin ışık mikroskobu ile değerlendirilmesi(Dicle Üniversitesi Tıp Fakültesi, 2008) Taşdemir, M. Serhan; Akkuş, Murat; Nergiz, Yusuf; Tunik, Selçuk; Kaplanoğlu, İskenderÇalışmamızda, bir statin türevi olan atorvastatinin sıçan iskelet kasında oluşturduğu yapısal değişikliklerin ışık mikroskobu düzeyinde incelenmesi ve değerlendirilmesi amaçlandı.40 adet erişkin erkek wistar albino rat; biri kontrol ve dokuzu deney grupları olmak üzere 10 eşit gruba bölündü. . Deneyin başlamasından sonra 5. 10. ve 15. günlerde sakrifiye edilen ratların quadriceps femoris kasları ışık mikroskobunda incelendi. Kontrol grubundan elde edilen kasların histopatolojik incelemelerinde herhangi bir yapısal değişikliğe rastlanmazken, diğer 9 deney grubundan elde edilen kas örneklerinde değişen derecelerde histopatolojik bulgular elde edildi. Genel olarak kaslarda dejenerasyon, kontraksiyon, nekroz, bağdoku artışı, mononükleer hücre infiltrasyonu, kas lifleri arasında vakuoler yapılar ve sentral nükleus belirgin artış göstermekteydi. Çalışmamızda bulgularımız, doz ve zaman arttıkça değişikliklerin belirgin olduğu gözlendi. Atorvastatinin iskelet kaslarında göstermiş olduğu toksik etki ile birlikte, klinik açıdan doz ve zaman baz alınarak, hasta tedavilerine ışık tutacağı kanaatine varıldı.Öğe Comparison of the results of sleeve gastrectomy, gastric pilication and liragulitide in obese rats(2023) Bilge, Hüseyin; Başol, Ömer; Yıldızhan, Eda; Ülger, Burak Veli; Temiz, Hakan; Akkuş, Murat; Yıldızhan, I.Obesity, which is generally seen in adults, is a serious health problem. Diseases caused by obesity are among the leading causes of death worldwide. Liraglutide (LG) is an analogue of glucagon-like peptide-1, which slows gastrointestinal motility, resulting in decreased food consumption. Gastric plication (GP) and sleeve gastrectomy (SG) is the reduction of stomach volume by surgical means. We examined and compared the body mass index (BMI) changes, metabolic changes and changes in gastric histology in obese rats after LG injection with surgical methods such as SG and GP. In this research, 35 Wistar Albino female rats were used. Rats were divided into 5 groups with 7 rats in each group. Group (G) 1: The control group, fed with a normal calorie diet for 8 weeks. G 2: Sham group, G 3: SG group, G 4: GP group and G 5: LG group, fed with high-calorie feed for 4 weeks. At the end of the 4th week, the study was terminated by making appropriate interventions for the groups. When the blood glucose (BG) levels measured at the beginning, 4th week and 8th week of the experiment were evaluated, it was monitored that the BG level at the 8th week was the lowest in the LG group (p<0.05). It was observed that the preop Ghrelin and Leptin levels of the LG group were lower than those of the SG and GP groups (p<0.05). As a consequence of our metabolic investigations, we observed that the use of LG is at least as effective as SG.Öğe Comparison of topical sucralfate with dexpanthenol in rat wound model(Wiley, 2022) Yıldızhan, Eda; Ülger, Burak Veli; Akkuş, Murat; Akıncı, Dilara; Başol, ÖmerWound healing is a dynamic process initiated in response to injury. There are many factors that have detrimental effects on the wound healing process. Numerous studies have been conducted for improving wound healing processes. Dexpanthenol is widely used to accelerate wound healing. Sucralfate is used for the treatment of peptic ulcers. We aimed to compare the efficacy of topical Dexpanthenol and Sucralfate in an experimental wound model in rats via histopathological examinations and immune histochemical determinations, as well, to evaluate their effects on EGF levels. Three different groups were formed: the Control Group, the Dexpanthenol Group and the Sucralfate Group. Full-thickness skin wounds were created on the back of each rat and isotonic saline was applied to the wounds of the rats in the control group, Bepanthol(R) cream was applied in Dexpanthenol Group and 10% Sucralfate cream was applied in Sucralfate Group, once a day. On the 7th, 14th and 21st days the wounds were measured and seven rats from each group were sacrificed and the wounds were excised for histopathological examination. Sucralfate increased wound healing rates by increasing neovascularization, fibroblast activation, reepithelialization and collagen density, as well as dexpanthenol. Our study revealed that the dexpanthenol and sucralfate groups were better than the control group in terms of their effects on wound healing, however there was no statistically significant difference among these two groups. Sucralfate improves EGF expression in skin wounds and has positive results on skin wound healing comparable to dexpanthenol.Öğe Could moesin be a new marker for indicating progression in endometrial cancer?(Dove Medical Press Ltd., 2022) Ağaçayak, Elif; Keleş, Ayşenur; Değer, Uğur; Özçelik, Mehmet Şirin; Peker, Nurullah; Gündüz, Reyhan; Akkuş, Murat; Büyükbayram, HüseyinAim: This study aims to determine an important parameter in progression from pre-invasive lesions of endometrium to endometrial cancer and also evaluate the effect of this parameter on the progression of endometrial cancer. Material and Method: In our study,30 patients with normal endometrial tissue (group 1), 56 patients who had endometrial hyperplasia without atypia (group 2), 36 patients who had endometrial hyperplasia with atypia (group 3), and 63 patients with endometrial cancer (group 4) were included. Age, parity, body-mass index, systemic diseases, and tumor markers of patients were evaluated. Expression levels of Ezrin, Radixin, and Moesin proteins were immunohistochemically evaluated in terms of frequency, intensity, and score value. Results: When we compared hyperplasia cases with or without atypia; frequency, and score value of ezrin expression and frequency, intensity, and score value of moesin expression was significantly higher in patients who had hyperplasia with atypia (p:0.000 p:0.001 p:0.003, p:0.032 p: 0.035 p:0.015 p:0.005, respectively). It was observed that the frequency and score value of moesin expression were significantly higher in patients with endometrial cancer when compared with patients who had hyperplasia with atypia (p:0.003 p:0.045). The frequency of moesin expression was significantly higher in patients who had postoperative mortality (p:0.030 p:0.039). Conclusion: Increased frequency of moesin expression in the preoperative period in patients with atypical hyperplasia should alert the surgeon in terms of malignancy. If the frequency of moesin expression increases in cases with endometrial cancer, the patient should be followed closely in terms of progression in the postoperative period.Öğe Değişik hayvan türlerinde açlığın ince barsaklar üzerine oluşturduğu histopatolojik değişiklikler(2018) Akkuş, Murat; Nergiz, YusufBu çalışmada 24,48,72,96 ve 120 saat aç bırakılan sıçan ve tavşanların ince barsakları incelenmiştir. Deneyin başında ve sonunda hayvanların vücut ağırlıklarına bakılmış ve ince barsak uzunlukları ölçülmüştür. Histopatolojik incelemeler için duodenumdan biopsiler alınarak %10'luk nötral formalinde fikse edildi. Elde edilen parafin kesitleri Hematoksilen-Eozin, Mallory-Azan ve PAS ile boyandı. Mikroskobik incelemede, açlık sonrası rat duodenumunda yer yer çizgili kenarda belirsizlik ve goblet hücrelerinin sayıca azaldığı gözlendi. Villuslarda ise çatallanma ve yarıklanma yanında apikalde tahribat, ayrıca kripta lümenlerinde belirsizlik ile lamina propria'da yer yer mononükleer hücre infiltrasyonu gözledik.Rat'ların duodenum mukozasında gözlenen bu histopatolojik bulgularla beraber, özellikle 96 ve 120 saat aç bırakılan tavşanların kripta lümenlerinde dilatasyon ve villusların lamina propria'sında hücre boşalması görüldü. İstatistiksel analiz sonucu erkek ve dişi rafların villus uzunlukları karşılaştırıldığında: 24 ile 120 saat aç bırakılan erkek rafların villus uzunlukları anlamlı iken (p<0.001) kripta derinlikleri açısından incelendiğinde 72 saat aç bırakılan dişi raflarda p değeri anlamlı bulundu (p<0.05). Sonuç olarak uzun süreli açlığın deney hayvanlarının barsak mukozasında pekçok dejeneratif değişikliklere neden olduğu kanaatine varıldı.Öğe The effect of high-fat diet on the development of obesity and serum leptin level in rats(Springer International Publishing, 1999) Bahçeci, Mithat; Tuzcu, Alpaslan Kemal; Akkuş, Murat; Yaldız, Mehmet Sadık; Özbay, A.Ten male Wistar-albino rats were overfed with high-fat diet (margarine at the dose 40 g/kg body weight/day in addition to standard rat diet) during six months from the age of between 8-12 weeks. Ten male rats received a standard chow for the same period. The body weights of the overfed rats increased time dependently (basal 171.5±5, at sixth month 268±19 g), whereas those of the rats fed with standard chow did not increase significantly (basal 177±6.4, at sixth month 220±10 g). At the sixth month, mean body fat percentages were 36.3±6.7% and 24.2±5.4% respectively. Both total cholesterol-triglyceride levels and mean serum leptin levels were also higher than in the overfed rats (p<0.01 and p<0.05 respectively). The livers of all rats were histopathologically normal. Conclusion: high-fat diet resulting in an increased body fat percentage in rats is associated with hyperleptinemia, hypercholesterolemia and hypertriglyceridemia.Öğe The effect of tamoxifen on the neonatal development of rat penis(1998) Deveci, Engin; Ketani, M. Aydın; Akkuş, Murat; Ersay, A. Reşit; Nergiz, Yusuf; Demir, Serap Sergül İnalözIn rats penis development a seen two stages in prenatal life, the second stage is androgen hormone dependent chondrocyte and osteocyte differentiation period. To investigate Tamoxifane (Tx) administration on the developmental anomalies of penis. The newborn male rats were injected with 100 ug Tamoxifen subcutaneously from the day of birth to fifth day. This changes in Os penis and glans penis were investigated after the rats had been sacrificed and their penis were removed on the days 14.0, 21.0, 28.0, 35.0 and 60.0 Having been fixed in neutral for-maline of 10%, the totally taken penis werembedded into paraffin blocks. The obtained paraffin sections were stained with Hematoxylene - Eosin, Verhoeff and Tripple. The structural changes in development of Os penis and glans penis were investigated. In our study, while formation of hyaline car-tilage, bone marrow and trabecula in proximal segment of Os penis was observed on the day 7.0 in the control group, a fibrocartilage tissue in distal segment of rats within control group was seen on days 28.0 - 60.0. In rats injected with Tx, it was observed that the hemapoetic tissue disappeared in the fourth week and the hylani ecartilage disappeared on the 60th day. It was also noticed that epi-dermal spindles in glans penis of rats injected with Tx as from the day 21.0 were gradually affected whereas epidermal spines and ker-atinization disappeared on the day 60.0. As result, we identified the blockade of the development of fibrocartilagoneus tissue and hyaline cartilage in Os penis and also the blockade of the maturation of epidermal tips in rats.Öğe Effectiveness of boric acid in preventing acrylamide-conducted brain damage in rats(Merthan Tunay, 2023) Yıldızhan, Eda; Ülger, Burak Veli; Gündüz, Ercan; Akkuş, Murat; Bilge, HüseyinAim: Acrylamide (ACR) is a water-soluble neurotoxic substance that has been widely researched in recent years. Boric acid (BA) is a component that does not have a toxic effect when taken at low concentrations and has a cystotoxic activity. Studies have reported that BA has antioxidant effects. In this study, we aimed to examine the protective efficacy of BA against the toxic damage that ACR may cause in the brain tissue. Methods: In this study, 28 Wistar Albino male rats with an average weight of 320-400 grams were used. In our study, ACR was administered intraperitoneally (i.p.) at a dose of 50 mg/kg for 14 days, while BA was administered orally (p.o.) with a dose of 200 mg/kg for 14 days. Group 1 (n=7): It is the control group and no medication was administered for 14 days. Group 2 (n=7): ACR group, Group 3 (n=7): BA group and Group 4 (n=7): ACR + BA group. Results: The comparison between the groups in terms of serum Total Oxidant Status (TOS) and Malondialdehyde (MDA) analysis revealed that the highest MDA level was in the ACR group. The MDA and TOS levels of the ACR+BA group were significantly lower than the ACR group (p<0.05). Conclusions: Our study revealed that BA has a protective effect in the prevention of neurotoxicity due to oxidative stress after ACR applicationÖğe Effects of antineoplastic agents on the peripheral nerves under a surgical tissue expansion procedure: An experimental study(Wiley, 2006) Oktay, M. Faruk; Aşkar, İbrahim; Yıldırım, Ayşe; Gürlek, Ali; Akkuş, Murat; Topçu, İsmail; Meriç, Faruk; 0000-0001-5708-4813Background. Elongation of peripheral nerve by the use of a tissue expander is helpful to repair nerve defects. This study was designed to investigate the effects of some antineoplastic agents on the peripheral nerves under a surgical tissue expansion procedure. Materials and Methods. Twenty-five Wistar rats were used in this study. Following the exposition of the sciatic nerve and placement of two 10/0-nylon sutures in the epineurium 20 mm apart, a tissue expander was then placed under it. Inflation of the expander was immediately accomplished by the separate percutaneous injections of 6, 6, and 8 ml for every 3 min under general anesthesia. The expander was fully deflated at the end of each 3 min The distance between two sutures was measured 1 h later to measure the rate of elongation. Rats were randomly divided into five groups (according to the administered drugs), each consisting of five rats (10 sciatic nerves). Normal saline (1 ml) in the control group (group 1), cyclophosphamide (15 mg/kg) in the group II, cisplatinum (3 mu g/kg) in the group III, mitomycin-C (0.5 mg/kg) in the group IV and 5-fluorouracil (10 mg/kg) in the group V were injected intravenously. Intravenous injections of drugs were performed via the tail vein 30 min before expansion, 48 and 96 h after removal of expander. The incision was reopened on the third and seventh postoperative days, and five sciatic nerves of each group were exposed and then the pinching test was performed to measure regeneration distance. Electro-neurographic changes were recorded. The expanded portion of the sciatic nerve between two sutures was harvested for histological evaluation. Results. There is no significant difference between the elongation rates of all groups (P < 0.05). Histologic evaluation showed that inflammatory changes, vacuolization, intraneural edema, demyelination, axonal changes in the control group, the cisplatinum group and the mitomycin-C group. These changes were significantly decreased in the cyclophosphamide group and the 5-fluorouracil group. In the cyclophosphamide group and the 5-fluorouracil group, the amplitude of compound action potential (CAP) values were significantly higher and the latency was significantly shorter (P > 0.05). Conclusion. We believed that cyclophosphamide and 5-fluorouracil may be helpful in tissue expansion of peripheral nerves, by decreasing the effects of the ischemia-reperfusion injury on the expanded peripheral nerves.Öğe The effects of clomiphene citrate administration in ovariectomised rats: An ultrastructural study(Asian Network for Scientific Information, 2008) Saruhan, Berna Güney; Ketani, Muzaffer Aydın; Nergiz, Yusuf; Ilgaz, Celal; Akkuş, Murat; Ketani, ŞennurIn this study, we aimed to investigate Clomiphene Citrate (CC) on vagen of adult Ovariectomised rats. Transmission electron microscopy and light microscopy have been used to study changes to the vaginal epithelium induced by C administered to Ovariectomised rats. The study was performed in Department of Medical Science Application and Research Centre of Dicle University, DUSAM-Turkey in 2001. Ten female, Wistar Albino rats were used and were divided into three groups. At the beginning and end of the administrations, body weight of animals was determined. Animals were sacrificed 35 days after ovariectomised and vaginal tissue was examined by transmission electron microscopy and light microscopy. We found that, the body weight was higher in the ovariectomized rats than the control rats. Further, body weight increased after clomiphene citrate therapy. It was found that clomiphene citrate treatment produced ultra structural surface features some of which were similar to those seen with hormone treatments and others unique to clomiphene alone. In conclusion, antiestrogen clomiphene citrate administration affects vagina epithelium and lead to increasing body weight.Öğe Effects of ischemia/reperfusion on ß cells of pancreas and protective effects of melatonin treatment(Universidad de la Frontera, 2009) Yıldırım, Ayşe; Tuncer, Mehmet Cudi; Pamukçu, Özlem; Aktaş, Ayfer; Akkuş, MuratOxygen free radicals are considered to be important components involved in the pathophysiological tissue alterations observed during ischemia-reperfusion (I/R). In this study, we investigated the putative protective effects of melatonin treatment on pancreatic I/R injury. Sprague Dawley male rats were subjected to 30 min of pancreatic pedicle occlusion followed by 90 min reperfusion. Melatonin (10 mg/kg. s.c) was administrated 30 min prior to ischemia or I/R application. At the end of the reperfusion periods, rats were decapitated. Pancreatic samples were taken for transmission electron microscopy. The results indicated that ischemia created b cell damage as evidenced by dilatation between the nucleus inner and outer membrane and degeneration on islets of Langerhans cells, was reversed by melatonin treatment. As melatonin administration reversed these microscopic damage, it seems likely that melatonin protects pancreatic tissue against oxidative damage.Öğe Effects of unilateral and bilateral epididymectomy on testes of rats(Dicle Üniversitesi Tıp Fakültesi, 2007) Aktaş, Ayfer; Nergiz, Yusuf; Akkuş, MuratIt is generally agreed that the testis is under endocrine control from the pituitary, and is influenced by physiological and paracrine factors within the organ. The aim of this study is to analyze the effect of unilateral and bilateral epididectomy on the testicular tissue growth of rats. Twenty- one male old Sprague-Dawley rats (28 days old) were used in the study. Rats were assigned into 3 equal groups. The first group was the control group, while unilateral and bilateral epididectomy was performed on the second and third groups, respectively. Twenty-one days after the epididectomy, testicular tissues from each group were taken and fixed in Bouin solution. Paraffin sections were stained with Haematoxylin and Eosin, Vangiesson, PAS-Hemalun and examined by light microscopy. Disorganization of the germinal epithelium, desquamation, degeneration and edema in interstitial tissue was seen in the testicular cross sections of the unilateral group. Arrest in spermatozoon stage in some tubules and presence of eosinophylic stained multinucler bodies were recognizable. In the bilateral group, degeneration and atrophic status in the seminiferous tubules of the bilateral group was observed preciesly, and occasional interstitial edema and perforations in the basal lamina were recognizable. In addition, vasodilatation, arrest in spermatozoa stage and multinucleated bodies in some of the seminiferous tubules lumen were observed in some testicular cross sections of this group. As a result, epididectomy causes degeneration in the germinal epithelium and hypoplasia in Leydig cells. It is concluded that epididectomy causes degeneration in the germinal epithelium, interruption of spermatogenesis, and a notable decrease in the number of Leydig cells.Öğe Ektodermal displazili olgularda konjonktiva bası sitolojisi(2012) Meşe, Ayşe; Söker, Sevda; Nergiz, Yusuf; Akkuş, Murat; Keklikçi, UğurEktodermal displazi ektodermal kökenli doku ve organları etkileyen bir belirtiler grubudur. Bu çalışmada ektodermal displazili olgularda konjonktiva etkilenmesini araştırmak amacıyla girişimsel olmayan bir yöntem olan bası sitolojisi tekniği kullanılmıştır. Dicle Üniversitesi Tıp Fakültesi Göz Hastalıkları ve Protetik Diş Sağlığı ve Hastalıkları Bilim Dallarında takipte olan ektodermal displazili üç hastanın altı gözü çalışma kapsamına alındı. Üst bulber konjonktivaya uygulanan bası sitoloji yöntemi ile selüloz asetat kağıdı yüzeyine alınan hücre grupları (%70’lik etil alkol, %37’lik formaldehit ve glisiyal asetik asitin 20:1:1 oranındaki) sıvısında sabitlendikten sonra “periodik acid schiff” ve hematoksilen-eozin ile boyandı. Ektodermal displazili olgularda bası sitolojisi ile hücre bütünlüğünde azalma, çekirdekte yer yer piknotik değişimler, epitelyal hücre şeklinde değişimler ve goblet hücre kaybı gözlendi. Ektodermal displazili olgularda göz kuruluğu bulguları bası sitolojisi tekniği ile desteklendi. Bası sitolojisinin, ektodermal displazinin göz izleminde, girişimsel olmayan bir yöntem olarak kullanılabileceği sonucuna varıldı.Öğe EXAMINATION OF APOPTOTIC CHANGES IN AROMATIC HYDROCARBON BENZENEADMINISTERED RAT TESTICULAR TISSUE WITH HISTOPATHOLOGICAL METHODS(2021) Aşır, Fırat; Özkorkmaz, Ebru Gökalp; Yıldızhan, Eda; Akkuş, Murat; Kaya, Nalan; Koyutürk, Leyla CanpolatBenzene is an aromatic hydrocarbon and a colorless and odorous liquid. It is used in the production of plastics, detergents, pesticides, and other chemicals. Serious deterioration is seen in sperm production of men who are exposed to hydrocarbons such as ethylbenzene, toluene, xylene, andaromatic solvents found in paints, lacquers, adhesive-like substances in their professions. The purpose of this study was to examine the possible effects of benzene on testicular tissues via histopathological and TUNEL staining methods. In this study, healthy 18 Wistar albino male rats, with a mean age of 8-10 weeks weighing 250-300 g were used. Rats were divided into 3 groups; Group I was the controlgroup. Group II was administered 1 ml/kg benzene for 9 days and Group III 1.5 ml/kg benzene for 5 days via orogastric tube. At the end of the experiment, all rats were decapitated, and their testicular tissues were excised. Stained with Haematoxylin and Eosin, Johnsen scoring was performed for each group. Apoptosis was demonstrated with TUNEL method, marked with the Image J program and statistical analysis was performed. Benzene exposed rat testicular tissues depicted thinning of seminiferous tubule epithelium cytoplasmic vacuoles, affusion of seminiferous tubule epithelium to the lumen, and affusion in spermatogenic cells (desquamation), degenerative changes in germ cells (spermatogonia), degenerative tubule structures, disorganized interstitial tissues, and absence of epithelial integrity were observed. Compared with the control group, the rats exposed to benzene revealed a significant increase in apoptotic cells in the seminiferous tubules via TUNEL staining. It was thought that exposure to benzene resulted in degeneration and increased apoptotic cells in the testicular tissues.Öğe Fertil kadınlar ve implantasyon başarısızlığı olan infertil kadınlarda endometriumun ince yapı ve immünohistokimyasal değerlendirilmesi(Dicle Üniversitesi Tıp Fakültesi, 2012) Bahar, Leyla; Akkuş, Murat; Baykal, Tülin; Kahraman, SemraAmaç: Endometrial reseptivite; endometrium epitelinin fonksiyonel başarısı olan geçici bir süreçtir. Bu çalışmada, fertil ve Tekrarlayan implantasyon başarısızlığı (TİB) olan bireylerin endometrium dokuları, Transmisyon Elektron Mikroskobuyla (TEM) ve E-cadherinle immünohistokimyasal açıdan değerlendirilmiştir. Amacımız, sadece fertil ve TİB olan kadınların endometrium dokularının karşılaştırılmasını yapmak değil aynı zamanda endometriumda implantasyon sürecinin hücresel çatısı ve mekaniğinin daha iyi anlaşılmasını sağlamaktır. Gereç ve yöntem: Araştırmamız için İn vitro fertilizasyon (İVF)/ Embriyo Transferi (ET) sonrası gebelik oluşmayan onyedi infertil ve on fertil kadın çalışmamıza dahil edilmiştir. Rutin ışık mikroskobu ve TEM teknikleriyle doku takibi sağlanmıştır. Bulgular: Fertil grubun endometrium yüzey epitelinde implantasyon belirteci pinopod oluşumuna yoğun olarak rastlanırken, infertil grupta yetersiz pinopod oluşumu ve belirgin silya ve mikrovillus varlığı dikkat çekmektedir. E-cadherin’le yapılan immünohistokimya çalışmasında skorlanma yapılmıştır. Fertil grup epitelinde immünreaktivite çok belirsizken, infertil grupta daha belirgin bir boyanma olduğu gözlenmiştir. Sonuç: Son bulgular E-cadherin’in reseptif dönemdeki endometrial epitele yapıştırıcı özellikler aktarıyor olması yanısıra, ikili fonksiyona sahip olması üzerinedir. İlk aşamalarda, hücre yüzeyinde yapışkanlık sağladığı, daha sonra epitel hücrelerde ayrılma yaptığı ve blastosistin invazyonunu etkinleştirmek için suprese olduğu düşünülmektedir. Bu çalışmada TİB grubunun aksine, fertil grup epitelinin, çok zayıf boyanması, blastosist implantasyonunu kolaylaştırması açısından önemlidir.Öğe The histopathological changes in rat femur that were fed with low-calcium diet in lactation and postlactation periods(1998) Ketani, M. Aydın; Nergiz, Yusuf; Şanlı, Ayfer; Güney, Berna; Akkuş, Murat; Deveci, EnginÇalışmamızda laktasyon ve laktasyon sonrası düşük kalsiyum diyetle beslenen ratların femur kemiğindeki histolojik değişiklikleri araştırdık. Araştırmamızda 20 tane erişkin 200-250 g ağırlığındaki Wistar albino rat kullanıldı. Kontrol grubundaki ratlar çiftleştirmeye alındıkları günden itibaren standart pelet yem ile beslendi (%0,5 Ca içeren). Bu gruptan elde edilen ratlar, laktasyon ve laktasyon sonrası periyotta da aynı diyetle beslendi. Deney grubundaki dişi ratlar, çiftleştirmeye alınarak gebelik döneminde standart pelet yem ile beslendi ( %0,5 Ca içeren). Deney grubunun yavru rafları laktasyon ve laktasyon sonrası düşük kalsiyum içeren ( %0.083 Ca içeren ) diyetle beslendi. Deney hayvanları 90.günde eter aneztezisı altında sakrifiye edilerek sağ femurları alınarak, %10'luk nötral forma/inde fikse edildi. Femurların dekalsifikasyonu % 5'lik formik asitle yapılarak, rutin histolojik takiplerle elde edilen 5u kalınlığındaki parafin kesitleri Hematoxylen-Eosin, Masson trichrome, Safranın f ast green ile boy andı. Yavru ratların femur kesitlerinde; kompakt kemikte deminerilizasyon alanları ve lamellerde düzensizlikler ile osteoklast sayısında artış gözlendi. Rezorpsiyon sonucu, kemik korteksi ve spongiöz trabeküllerde belirgin bir incelme saptandı.Öğe INVESTIGATION OF GLOBOZOOSPERMIA’S MORPHOLOGICAL STRUCTURE AND DNA FRAGMENTATION IN OLIGOZOOSPERMIA CASES IN INFERTILE MALES(2022) Yıldızhan, Eda; Çankırı, Zuhal; Akıncı, Dilara; Aşır, Fırat; Afşin, Muhamet; Akkuş, Murat; Dede, OnurTotal globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa without acrosomes. It is still unclear whether patients whose ejaculate contains both normal and globozoospermic cells (partial globozoospermia) suffer from a variation of the same syndrome. Affected men may experience decreased fertility and even infertility. In some cases, an increased number of cells with DNA fragmentation has also been observed in patients with globozoospermia..In this study, standard semen analysis methods in accordance with WHO criteria were applied to infertile male patient groups consisting of 20 normozoospermic and 20 oligozoospermic individuals who were admitted to our clinic. Age, sperm parameters (volume, vitality, concentration, total motility and morphology) were determined and statistically analyzed in normozoospermic and oligozoospermic infertile men.Sperms were stained with the Eosin-Nigrosin method and were visualized under an immersion lens light microscope and evaluated for vitality. The slides were stained using sperm staining solutions with the Spermac technique and the sperms were evaluated morphologically. Sperm DNA fragmentation damage was evaluated by acridine orange staining method.Our results revealed that sperm morphological features (Kruger test) and sperm DNA fragmentation, obtained with various staining techniques, are important in the clinical approach to male infertility and ART methods, and should be used together.Öğe INVESTIGATION OF SFLT-1 AND VEGF EXPRESSION IN NORMOTENSIVE AND PREECLAMPTIC PLACENTA. AN IMMUNOHISTOCHEMICAL STUDY(Rojan GÜMÜŞ, 2020) Sahin, Firat; Akkuş, Murat; Şeker, Uğur; Soker, Sevda; Gokalp-ozkorkmaz, Ebru; Ağaçayak, Elif; Aşır, FıratObjectives: The pathogenesis of preeclampsia is still not clear, but endothelial dysfunction believed to be one of the most encountered problems during placenta development in preeclamptic patients. Both vascular endothelial growth factor (VEGF) and its antagonist, soluble Fms-Like tyrosine kinase-1 (sFlt-1), have roles in vascular function. In this study, we have investigated immunohistochemical expression of VEGF and sFlt-1 in term placenta of normotensive and preeclampsia patients. Methods: Totally twenty term placentas were obtained from pregnant women of which 10 preeclampsia patient and 10 normotensive. Placentas were dissected and tissue samples were subjected to routine tissue processing protocol, then embedded in paraffin blocks. Serial sections were obtained from paraffin blocks and stained with H&E and PAS for routine histopathology. VEGF and sFlt-1 immunohistochemistry was performed to the sections. Results: When compared to control group, severe pathological changes were observed in preeclamptic placentas. Increase in number of syncytial knots and intervillous bridges, hemorrhage in interstitium, dilatation and congestion in villous capillaries, increase in fibrin accumulation in villus stroma, and increase in thickening of basement membrane were very clear. VEGF expression was significantly higher in normotensive placentas compared to preeclamptic. On the other hand, sFlt-1 expression was significantly increased in preeclamptic placentas. Conclusions: When the VEGF and sFlt-1 expression was considered, higher expression of sFlt-1 at preeclampsia, but decrease in VEGF expression, might be related to endothelial dysfunction in preeclampsia. Overall, this study demonstrates, imbalance between VEGF and sFlt-1 is one of the major reason of endothelial dysfunction in preeclamptic placenta.Öğe Investigation of the protective effect of boric acid against hepatotoxic and nephrotoxic injury induced by acrylamide in rats(Universidad de la Frontera, 2023) Gündüz, Ercan; Yıldızhan, Eda; Yaman, Mahmut; Şen, Abdullah; Akkuş, MuratTo investigate if the administration of boric acid (BA) would exert any protective effect against possible nephrotoxicity and hepatotoxicity induced by the exposure to acrylamide (ACR) in rats. In our study, we used a total of 28 rats that were divided into four equal groups. Group 1: the control group which was not treated with any procedure. Group 2: the ACR group that was administered ACR 50 mg/kg/day via intraperitoneal (i.p) route for 14 days. Group 3: the BA group that was administered BA 200 mg/kg/ day via gavage via peroral (p.o) route for 14 days. Group 4: the ACR+BA group that was administered BA simultaneously with ACR. Total antioxidant and oxidant (TAS/TOS) capacities were measured in all groups at the end of the experiment. In addition, the specimens obtained were evaluated with histopathological examination. Studies showed that the ACR and ACr+BA groups were not significantly different in terms of hepatic TAS level while the TOS level was higher in the ACR group than the ACR+BA group. The groups did not show any significant difference regarding renal TAS and TOS levels. In the histopathological examination of the hepatic tissue, the histopathological injury score of the ACR group was significantly higher than those of the other groups whereas it was significantly lower in the ACR+BA group than the ACR group. Our study concluded that Boric acid had a protective effect against acrylamide-induced hepatotoxicity, but not against nephrotoxicity.Öğe Konjenital blefaroptozisli olgularda levator palpebra superior kasının histolojik değerlendirmesi(Dicle Üniversitesi Tıp Fakültesi, 2011) Söker, Sevda; Çakmak, Sevin; Akkuş, Murat; Nergiz, YusufAmaç: Bu çalışmanın amacı konjenital blefaroptozisli olgularda levator palpebra superior kasının histolojik olarak incelenmesi ve elde edilen bulguların hastaların yaş, cinsiyet ve blefaroptozis derecesi ile olan ilişkisinin araştırılmasıdır. Gereç ve yöntem: Ocak 2009- Ocak 2010 tarihleri arasında Dicle Üniversitesi Tıp Fakültesi Göz kliniğine başvuran ve levator palpebra superior kası rezeksiyonu uygulanan 13 konjenital ptozisli olgunun levator kası Dicle Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji bölümünde histopatolojik incelemeye alındı. Ameliyat öncesi dönemde, ptozis miktarı, levator fonksiyonu (LF), göz yaşı fonksiyonları, Bell fenomeni ve jaw-winking fenomeni değerlendirildi. Tüm olgulara levator palpebra superior kas rezeksiyonu uygulandı. Ameliyat sonrası alınan levator kası histolojik olarak ışık mikroskobi ile incelendi. Bulgular: Olguların yaşları ortalama 10.61 ± 4.77 (4-19) yıl olup, 9 (% 69.2)’u erkek, 4 (% 30.8)’ü kız idi. Histolojik incelemede levator kas fibrillerinin nitelik ve niceliği değerlendirildi. Olgularda yaş, cinsiyet ile levator kasının histolojik özellikleri arasında ilişki saptanmadı (p>0.05). Levator palpebra superior kası zayıf olan olgularda levator kasında histolojik olarak yağlı dejenerasyon varlığı saptandı. Levator palpebra superior kas fonksiyonu arttıkça yağlı dejenerasyonda azalma çizgili kas fibrillerinde artış görüldü. Sonuç: Konjenital blefaroptozisli olguların levator kas yapısı ile levator palpebra superior kas fonksiyonu arasındaki ilginin daha geniş serilerde ve ultrastrüktürel çalışmalarla desteklenmesi gerekir.
