Yazar "Özkorkmaz, Ebru Gökalp" seçeneğine göre listele
Listeleniyor 1 - 16 / 16
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomised rats with tamoxifen citrate administration(Via Medica, 2019) Baloǧlu, Murat; Özkorkmaz, Ebru GökalpBackground: Osteoporosis results with the imbalance between osteoblastic formation and osteoclastic resorption, resulting in susceptibility to bone fractures. Ovariectomy leads to osteoporosis by triggering alterations in bone formation and structure. Tamoxifen as an anti-oestrogen is used for adjuvant therapy especially in metastatic diseases and known to have a bone mass protective effect after ovariectomy. Materials and methods: An animal model of ovariectomy induced osteoporosis after tamoxifen citrate administration was studied via biochemical and immunohistochemical methods. Female Wistar albino rats (n = 45), selected according to their oestrous cycle, were divided into three groups; I - control, II - ovariectomy, III - ovariectomy + tamoxifen. Following ovariectomy, tamoxifen citrate (10 mg/kg) was given intraperitoneally daily for 8 weeks. At the end of the period, animals were sacrificed under anaesthesia, blood samples were taken to measure oestrogen, calcium, and alkaline phosphate. Tibia bone samples were fixed in formalin solution and decalcified with 5% ethylene-diamine tetra acetic acid. After the routine histological follow up, samples were embedded in paraffin and cut with a microtome for semi-thin sections. Primary antibodies osteonectin and osteopontin were applied to sections and examined under light microscope. Results: As a consequence, when oestrogen and calcium data were compared there was a decrease in ovariectomy group with an increase in alkaline phosphatase. In ovariectomy + tamoxifen group, these values were close to the control group. Osteonectin was observed to promote bone formation by influencing collagen fibre formation, extracellular matrix development, osteoblast differentiation and the capacity to affect osteoclast activity. Conclusions: It has been suggested that osteopontin, the cytokine and cell binding protein, stimulates cellular signalling pathways, induces bone remodelling and acts in osteoporosis.Öğe Diyabetik Ayak Yaraları Üzerine İmmunohistokimyasal Bir Çalışma; MMP-2 ve TNF- ? Ekspresyonlarının İncelenmesi(2019) Özkorkmaz, Ebru Gökalp; Baloğlu, MuratAmaç: Diyabetik ayak, diyabetin önemli ve uzun süreli komplikasyonlarından biridir. Bilindiği üzere diyabetik bireylerde yara iyileşmesi yavaş olmaktadır ve bu duruma bakteriyel invazyonun eklenmesi sonucu uzun süreli inflamasyon eşliğinde iyileşmeyen diyabetik ayak yaraları ortaya çıkmaktadır. Söz konusu çalışmanın amacı, diyabetik ayak yara dokusunda proinflamatuvar sitokinlerden TNF-? ve kollajenin parçalanmasında rol oynayarak dokunun yeniden şekillenmesini sağlayan matriks metaloprotein MMP-2 ekspresyonunu immunohistokimyasal yöntemlerle tespit etmektir. Materyal ve Metot: Bu çalışmaya 30 erkek ve 30 kadın olmak üzere, diyabetik ayak tanısı almış, ve ayaklarında açık yara bulunan 60 birey dahil olmuştur. Çalışmaya alınacak ayak, izotonik çözelti ile yıkandıktan sonra yaralar kesilip çıkarılmış ve dokular %10’luk formaldehit solüsyonunda tespit edilmiştir. Rutin histolojik takip sonrası kesitler parafine gömülmüş ve yarı- ince kesitleri alınarak histopatolojik incelemeleri yapılmıştır. İmmunohistokimyasal analiz için, doku örnekleri, MMP-2 ve TNF- ? primer antikorları ile boyanarak mikroskop altında incelenmiştir. Bulgular: Çalışmamızın sonuçlarına göre diyabetik ayak yara dokusunda, ligamenter dokunun içinde lökositler, lenfositler ve monositlerin yoğun olduğu izlenmiştir. Kollajen liflerde dejenerasyon ve kan damarlarında dilatasyon, konjesyon ve ödem görülmüştür. İnflamatuvar hücrelerde ve nekroze olan alanlarda TNF-? ekspresyonunda artış izlenmiştir. Damar çevresinde görülen yoğun inflamasyonunun arasında, dejenere kollajen lif ve fibroblast hücreleri ve ekstrasellüler matrikste MMP-2 ekspresyonu pozitif olarak gözlenmiştir. Sonuç: Diyabetik ayak yarası tedavisinde MMP ekspresyonu yönünde düzenleme yapılarak, her geçen gün genişleyen diyabetik popülasyonda iyileşmeyen ayak yaralarına karşı bir yaklaşım geliştirilebilir düşüncesindeyiz.Öğe The effect of graft application and allopurinol treatmenon calvarial bone defect in rats(Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia, 2019) Laçin, Nihat; İzol, Bozan Serhat; Özkorkmaz, Ebru Gökalp; Deveci, Buşra; Tuncer, Cudi Mehmet;Purpose: To investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material. Methods: Thirty-six Wistar albino rats were divided into 3 groups. In the control group, calvarial bone defect was only created without any treatment. In the Defect + Graft group, allograft treatment was performed by forming 8 mm calvarial bone defect. In the Defect + Graft + Allopurinol group, alloplastic bone graft was placed in the calvarial bone defect and then, allopurinol (50 mg/kg/day) treatment was intraperitoneally applied for 28 days. Results: Histopathological examination revealed inflammation, congestion in the vessels, and an increase in osteoclast cells in the defect area. We also observed that new osteocyte cells, increase in connective tissue fibers, and new bone trabeculae. Osteopontin expression was positive in osteoblast cells and lacunated osteocyte cells were located in the periphery of the new bone trabeculae. Osteopontin expression was also positive in osteoblasts and osteocytes cells of new bone trabeculae in the graft site. Conclusion: It has been shown that allopurinol treatment in rat calvaria defects may induce osteoblastic activity, matrix development, mature bone cell formation and new bone formation when used with autogenous graftsÖğe The effect of graft application and simvastatin treatment on tibial bone defect in rats. A histological and immunohistochemical study(Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia, 2019) Laçin, Nihat; İzol, Bozan Serhat; Özkorkmaz, Ebru Gökalp; Deveci, Buşra; Tuncer, Cudi MehmetPurpose: To evaluate histologically and immunohistochemically the bone regeneration after application of simvastatin on tibial bone defects in rats. Methods: Sixty Wistar albino rats were divided into 3 groups as control (6 mm tibial bone defect), defect + graft (allograft treatment), and defect + graft + simvastatin (10 mg/kg/day) for 28 days. Results: Histopathological examination revealed inflammation in control group (defect group), congestion in blood vessels, and an increase in osteoclast cells. In defect + graft group, osteoclastic activity was observed and osteocyte cells were continued to develop. In defect + graft + simvastatin group, osteocytes and matrix formation were increased in the new bone trabeculae. Osteopontin and osteonectin expression were positive in the osteclast cells in the control group. Osteoblasts and some osteocytes showed a positive reaction of osteopontin and osteopontin. In defect + graft + simvastatin group, osteonectin and osteopontin expression were positive in osteoblast and osteocyte cells, and a positive expression in osteon formation was also seen in new bone trabeculae. Conclusion: The simvastatin application was thought to increase bone turnover by increasing the osteoinductive effect with graft and significantly affect the formation of new bone.Öğe EXAMINATION OF APOPTOTIC CHANGES IN AROMATIC HYDROCARBON BENZENEADMINISTERED RAT TESTICULAR TISSUE WITH HISTOPATHOLOGICAL METHODS(2021) Aşır, Fırat; Özkorkmaz, Ebru Gökalp; Yıldızhan, Eda; Akkuş, Murat; Kaya, Nalan; Koyutürk, Leyla CanpolatBenzene is an aromatic hydrocarbon and a colorless and odorous liquid. It is used in the production of plastics, detergents, pesticides, and other chemicals. Serious deterioration is seen in sperm production of men who are exposed to hydrocarbons such as ethylbenzene, toluene, xylene, andaromatic solvents found in paints, lacquers, adhesive-like substances in their professions. The purpose of this study was to examine the possible effects of benzene on testicular tissues via histopathological and TUNEL staining methods. In this study, healthy 18 Wistar albino male rats, with a mean age of 8-10 weeks weighing 250-300 g were used. Rats were divided into 3 groups; Group I was the controlgroup. Group II was administered 1 ml/kg benzene for 9 days and Group III 1.5 ml/kg benzene for 5 days via orogastric tube. At the end of the experiment, all rats were decapitated, and their testicular tissues were excised. Stained with Haematoxylin and Eosin, Johnsen scoring was performed for each group. Apoptosis was demonstrated with TUNEL method, marked with the Image J program and statistical analysis was performed. Benzene exposed rat testicular tissues depicted thinning of seminiferous tubule epithelium cytoplasmic vacuoles, affusion of seminiferous tubule epithelium to the lumen, and affusion in spermatogenic cells (desquamation), degenerative changes in germ cells (spermatogonia), degenerative tubule structures, disorganized interstitial tissues, and absence of epithelial integrity were observed. Compared with the control group, the rats exposed to benzene revealed a significant increase in apoptotic cells in the seminiferous tubules via TUNEL staining. It was thought that exposure to benzene resulted in degeneration and increased apoptotic cells in the testicular tissues.Öğe Immunoexpression of vascular endothelial growth factor, ?-cell lymphoma 2 and cluster of differentiation 68 in cerebellar tissue of rats treated with Ganoderma lucidum(Universidad de la Frontera, 2018) Özevren, Hüseyin; İrtegün, Sevgi; Ekingen, Arzu; Tuncer, Cudi Mehmet; Özkorkmaz, Ebru Gökalp; Deveci, Engin; Deveci, ŞenayTraumatic brain injury (TBI) can potentially lead to hemorrhages in all areas of the skull, which can damage cells andnerve connections. This study aims to investigate the protective effects of Ganoderma lucidum polysaccharides (GLPS) as a antioxidant oncerebellar cell tissues after traumatic brain injury in rats. Sprague Dawley rats were subjected to TBI with a weight-drop device using 300 g-1m weight-height impact. The groups are consisted of control, trauma, and trauma+Ganoderma lucidum groups. At seven days post-braininjury, experimental rats were decapitated after intraperitoneal administration of ketamine HCL (0.15 ml/100 g body weight). Cereballarsamples were taken for histological examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels andmyeloperoxidase (MPO) activity. Significant improvement was observed in cells and vascular structures of Ganoderma lucidum treatedgroups when compared to untreated groups. It is believed that Ganoderma lucidum may have an effect on the progression of traumatic braininjury. Ganoderma lucidum application may affect angiogenetic development in blood vessel endothelial cells, decrease inflammatory cellaccumulation by affecting cytokine mechanism and may create apoptotic nerve cells and neuroprotective mechanism in glial cellsÖğe IMMUNOHISTOCHEMICAL ANALYSIS OF SFLT-1 AND ADAMTS-8 EXPRESSION IN DIABETIC RAT TESTIS TISSUE(Rojan GÜMÜŞ, 2019) Kaya, Seval; Nergiz, Yusuf; Asır, Fırat; Özkorkmaz, Ebru Gökalp; Erdogan, Gamze; Deveci, EnginAbstract: The aim of this study was to determine the testicular inflammation and angiogenetic effect of diabetes with ADAMTS-8 and SFlt-1 proteins immunohistochemically. Wistar albino male rats (n: 12)were used for the study, Group 1: Control group (n: 6), only 1 ml i.p. saline injection was performed. Group 2: Diabetes group (n: 6) received a single intraperitoneal dose of streptozotocin (STZ) of 60 mg / kg. The glucose value measured above 250 mg / dl was considered as diabetic. Under anesthesia, dissection was performed at the lower part of abdominal clearance and testicular tissue was removed. Testis tissues were fixed in 10% neutral formalin, followed by the routine paraffin protocol and cut with a microtome. Then, primary antibodies (ADAMTS-8, sFlt-1) were applied by immunohistochemistry method and incubated at +4 °C overnight. The sections were then examined under a light microscope. Diabetes group showed that Leydig cells in the inter tubular area had vacuolization and capillary dilatations in histopathological examinations. sFlt-1 staining of control group showed positive expression in capillary endothelium between Leydig chambers of the intertubular area. Sflt-1 expression of diabetes group was observed in degenerative spermatic cells and Sertoli cells of the basement membrane facing tubules. The expression of ADAMTS-8in control group was positive in some Leydig cells in the interstitial area of Sertoli cells in seminiferous tubule but, spermatogenetic cells were negative. In the ADAMTS-8 staining of diabetes group, the expression of ADAMTS-8 was increased in the stromal cells and some inflammatory cells in the intertubular space. SFlt-1 plays a crucial role in angiogenesis as well as in diabetic testes and is marked as a precursor for the disruption of vascular structure and blood flow due to degenerative changes. It is thought that the distribution of ADAMTS-8 may be a determinant protein in the development of extracellular matrix and in damage to testicular tissue of diabetic testis.Öğe Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-k? Protein Ekspresyonu(2019) Baloğlu, Murat; Özkorkmaz, Ebru GökalpAmaç: Menisküs zorlanmalara karşı emici özelliği ile eklem stabilitesini sağlar. Menisküs yırtığı ise yaşam kalitesini düşüren ciddi bir travmadır. Bu çalışmada, menisküs yırtığı durumunda sinoviyal sıvıdaki hücrelerde meydana gelen farklılıkların immünohistokimyasal yöntemlerle incelenmesi amaçlanmıştır. Gereç ve Yöntem: Menisküs yırtığı olan 28 erkek ve 32 kadın hasta çalışmaya dahil edilmiştir. Hastaların fizik muayenede lokal ağrı hissi, şişme, günlük aktivitelerini yerine getirmekte ve koşma-yürümede güçlük gibi şikayetleri olduğu belirlendi. Hastaların manyetik rezonans görüntülemeleri (MRG) değerlendirildi. Menisküs ağrısı olan dizlerin yanal suprapatellar poş kısmından enjektör ile 5 cc sinoviyal sıvıları aspire edildi. Örneklerin rutin histolojik takipleri yapıldıktan sonra parafine gömüldü ve mikrotom ile yarı-ince kesitleri alındı. İmmunohistokimyasal analiz için matriks metaloprotein (MMP-2) ve nükleer faktör kappa (NF-k?) primer antikorları kullanıldı. Kesitlerin şık mikroskobu ile fotoğrafları çekildi ve değerlendirildi. Bulgular: MRG sonucu eklem içerisinde mayi artışı ve parçalı yırtık izlendi. Kadın hastaların eklem sıvılarında lenfosit ve nötrofil hücrelerinde ve hiperplastik eritrositler ile fibroblast sayısında artış gözlendi. Erkek hastalarda, fibroblast hücreleri hiperplastik olarak izlendi ve plazma hücrelerinin arttığı görüldü. MMP-2 ekspresyonunun plazma hücrelerinde, fibroblastlarda ve nötrofillerde arttığı ayrıca, NF-k? ekspresyonunun lenfosit ve polimorfik çekirdeğe sahip hücrelerde pozitif olduğu izlendi. Sonuçlar: Menisküs yırtığı ve enflamasyonu olan eklemlerde artmış sıvının kıkırdak matriksinde hasara neden olduğu ve buna bağlı olarak MMP-2 ve NF-k? gibi sitokinlerin ekspresyonunun arttığı, fibroblast hücreleri gibi sentez yapan hücrelerin yıkıma uğradığı düşünülmektedir.Öğe Neuroprotective effects of allopurinol on spinal cord injury in rats: A biochemical and immunohistochemical study(Via Medica, 2019) Baloǧlu, Murat; Özkorkmaz, Ebru GökalpBackground: Lesion in spinal cord causes a cascade of events such as the apoptosis of neurons and eventually, neurological dysfunction. Neurologic damage developing after acute spinal cord injury is also related with necrosis and free radical formation. Allopurinol, a xanthine oxidase inhibitor, was shown to have protective effects in several studies. B-cell lymphoma 2 (Bcl-2) family proteins regulate apoptosis. Apoptosis causes the death of neuronal cells, particularly neurons and oligodendrocytes in the spinal cord after lesion. Glial fibrillary acidic protein (GFAP) takes part in astrocyte and neuronal interconnection and synaptic transmission. Materials and methods: Male Sprague Dawley rats (n = 30) were divided as control, trauma, and trauma + allopurinol (i.p., 50 mg/kg of body weight) groups. Animals were applied a surgical procedure causing spinal cord injury and treated for 7 days then sacrificed under anaesthesia. The spinal cords were dissected, measurements of myeloperoxidase, malondialdehyde and glutathione were performed, remaining parts were fixed in 10% formaldehyde solution for histological and immunohistochemical evaluations. Results: Biochemical results exhibited an increase in myeloperoxidase levels in trauma group but a decrease in the allopurinol treatment group similar to malondialdehyde levels. Degenerative changes in multipolar and bipolar neurons together with apoptotic changes in some glial cells were observed in the trauma group whereas, mild degenerative changes were observed after allopurinol treatment. In the trauma group, negative GFAP expression in multipolar versus bipolar neuronal processes with a reduction in glial processes around blood vessels and positive GFAP expression were observed but, a regular and parallel positive GFAP expression of glial processes around blood vessels in the allopurinol treated group was apparent. Trauma group depicted a positive Bcl-2 expression in glial cells and in motor and bipolar neurons. On the contrary, negative Bcl-2 expression was noticed in the trauma + allopurinol group. Conclusions: This study is of importance to understand the effects of allopurinol in preventing degenerative changes in nerve and glial cells related to spinal cord injuries.Öğe OVAREKTOMİZE SIÇANLARDA KAN VE TİBİA KEMİK DOKULARINDA AKUT DÖ-NEMDE MEYDANA GELEN DEĞİŞİKLİKLERİN BİYOKİMYASAL, HİSTOPATOLOJİK VE İMMÜNOHİSTOKİMYASAL YÖNDEN DEĞERLENDİRİLMESİ(2020) Özkorkmaz, Ebru Gökalp; Baloğlu, MuratAMAÇ: Menopoz sonrası meydana gelen osteoporoz, kemik kütle-sinde azalma ve kemik dokunun yapısında bozulma ile sonuçlanır. Ovariektomi sonrası, osteoporoz bulgularına rastlanmakta ve ke-mik yıkımı kemik oluşumundan fazla olduğundan kemik kaybına neden olmaktadır. ADAMs transmembran proteinleri ekstrasellüler matriksin yeniden yapılandırılmasında ve embriyonik gelişim, hüc-re göçü, adezyon, anjiyogenez olmak üzere birçok fizyolojik süreçte rol alırlar. Çalışmamızın amacı, ovariektomi oluşturulan sıçanlarda kan ve kemik dokusunda akut dönemde meydana gelen değişikleri biyokimyasal, histopatolojik ve immünohistokimyasal yöntemlerle araştırmaktır. GEREÇ VE YÖNTEM: Sağlıklı ve dişi 20 adet erişkin Wistar albino sı-çan kontrol (n:10) ve bilateral ovariektomi uygulanan grup (n:10) ol-mak üzere 2 gruba ayrıldı. Anestezi altında yumurtalık rahim boynu kısmından 2 cm'lik kesi yapılarak, iki dorsolateral insizyon ile uterus apeksinden çift taraflı yumurtalıklar ipek iplikle çıkarıldı. Ovariekto-mi den 1 hafta sonra, deney hayvanları feda edildi. Serum östrojen, kalsiyum ve alkalin fosfat değerlerini ölçmek için her hayvandan kan örnekleri alındı, tibia kemikleri çıkarılarak formalin çözeltisine alına-rak tespit edildikten sonra, dekalsifiye etme işlemine tabi tutuldu ve rutin histolojik işlemlerin ardından parafin blokları hazırlandı, ya-rı-ince kesitleri alınarak histopatolojik incelemeler için Hematoksilen Eozin boyası ile boyandı. İmmünohistokimyasal analiz için, antijen retrival işlemini uygulamak üzere sitrat tamponuna alındıktan sonra hidrojen peroksit ile muamele edildi. Kesitlere anti-ADAMTS-1 pri-mer antikoru uygulandı, ışık mikroskobunda değerlendirildi.BULGULAR: Kontrol grubunda radiyal tarzda dağılmış havers kanal-ları ve volkman kanalları izlenirken, kollajen lifler birbirine paralel ve düzenliydi. Ovariektomi grubunda ise kemik matriksinde dejenera-tif değişiklikler, lamelli kemik yapısında bozulma izlendi. ADAMTS-1 ekspresyonu açısından, kontrol grubu osteoblast ve osteosit hücre-lerinde ve kemik matriksindeki ince kollajen liflerde ekspresyon po-zitifti. Ovariektomi grubunda, dejeneratif alanda yer alan osteoklast hücrelerinde ADAMTS-1 ekspresyonunda artış, osteon yapılarında, endotel hücrelerinde ADAMTS-1 pozitif ekspresyonu izlendi. Kanda östrojen değerleri açısından, ovariektomi grubunda kontrol gru-buna göre anlamlı bir düşüş gözlendi.Kalsiyum değerlerinde ise kontrol ve ovariektomigruplarının değerleri birbirine yakındı. Al-kalen fosfataz değerlerinin ovariektomi grubunda yükseldiği görül-dü(p=0,01). SONUÇ: Çalışmamız sonucu elde ettiğimiz veriler ışığında, osteo-poroz tedavisine ADAMTS-1 proteinlerinin dahil edilmesinin yeni tedavi yöntemlerini destekleyebileceği düşüncesinÖğe Prolonged simvastatin treatment provided a decrease in apoptotic, inflammatory, and oxidative stress markers in ischemia-reperfusion-induced acute kidney injury model of rats(Sci Printers & Publ INC, 2021) Kafkaslı, Alper; Özkorkmaz, Ebru GökalpOBJECTIVE: Ischemia-reperfusion (I/R) leads to reactive oxygen species formation and cell death in kidney tissue with injury and organ transplantation. Simvastatin (SIM) is an antioxidant, anti-inflammatory, and anticoagulant agent. Alterations in I/R-induced acute kidney injury model with SIM treatment were analyzed. STUDY DESIGN: Wistar rats (n=28) were grouped into Sham, Ischemia, I/R, and I/R+ SIM treated. Left rat kidney renal vessels were clamped for 60 minutes for ischemia, and the I/R group had 6 hours of reperfusion. 10 mg/kg SIM was given orally for 28 days. MDA, GSH, and MPO were analyzed. Kidney tissues were paraffin embedded, and primary antibodies TNF-alpha and caspase-3 were applied for immunohistochemistry. RESULTS: In the I/R group, intense inflammatory cell infiltration around the vessels and necrosis in the glomerular structures were observed. In the treated group, proximal and distal tubular cells were found to be close to normal. Immunoexpression of caspase-3 in the ischemia group was positive in degenerative glomeruli. In the treated group, TNF-alpha expression was negative in the glomerular structures. MDA and MPO levels were significantly increased in ischemia and I/R. CONCLUSION: We suggest that SIM treatment improved kidney tissue structure and function in a model of I/R injury.Öğe Protective effects of Silymarin on testicular torsion/detorsion in rats(2023) Azizoǧlu, Mustafa; Arslan, Serkan; Özkorkmaz, Ebru Gökalp; Aşır, Fırat; Basuguy, Erol; Okur, Mehmet Hanifi; Aydoğdu, Bahattin; Karabel, M. Alagöz; Kaplan, İbrahimOBJECTIVE: The present research aimed to study the possible protective effects of Silymarin on testicular I/R injury in a rat model evaluated through histopathology and biochemical parameters. MATERIALS AND METHODS: This research investigated the impact of Silymarin on IR damage in male Wistar albino rats. Animals were divided into three groups: group 1 (sham), group 2 (IR), and group 3 (IR+Silymarin). RESULTS: There were no notable differences in the levels of malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) across the three groups (p=0.260, p=0.486 and p=0.803, respectively). Contrarily, the total antioxidant status (TAS) levels exhibited significant variations between groups (p=0.001). The total oxidant status (TOS) levels also differed significantly between groups (p=0.004). The tissue evaluations uncovered substantial differences in the Johnson score, which is used to gauge testicular damage. A distinct contrast was seen between Group 1 and Group 2, and also between Group 2 and Group 3, with an all-encompassing p-value lower than 0.01. The same significant disparities were found for the percentages of Bax and Annexin V immunostaining (p<0.01 for each), reflecting the inflammation and apoptosis brought about by ischemia-reperfusion and the protective effects of the treatment. CONCLUSIONS: The outcomes of the current investigation showed that Silymarin could be a valuable agent for reducing testicular tissue damage following I/R injury.Öğe Rosmarinic Acid Ameliorates Deltamethrin Induced Hepatotoxicity and Nephrotoxicity(Tıbbi Kayıtlar Derneği, 2023) Yildizhan, Eda; Ülger, Burak Veli; Akkuş, Murat; Katanalp, Ömer Faruk; Özkorkmaz, Ebru Gökalp; Yıldızhan, İbrahimAim: Deltamethrin (DM) is an insecticide and is widely used around the world. Rosmarinic acid (RA) is found in herbs and spices in the Lamiaceae (mint) family and has antioxidant, antiinflammatory and antiapoptotic effects. We objective to examine the protecting efficacy of Rosmarinic acid in preventing the toxic effects of Deltamethrin. Material and Methods: In ours study we used 28 male rats. Group 1: Control group, Group 2: RA group, RA was given 20 mg/kg peroral (p.o.) for 7 days, Group 3: DM group, 35 mg/kg/dose of DM was given 24 hours before sacrification as a single dose by gavage, Group 4: RA+ DM group. Results: BUN, creatinine, AST and ALT values of the RA+DM group were lower than the DM group. TAS and TOS grades were higher in the DM group matched to the RA+DM group. The damage scores of the DM group were higher aacording as those of the RA+DM group. Conclusion: RA has been shown to have predicative influence in the therapy of deltamethrin-induced nephrotoxicity and hepatotoxicity.Öğe Rosmarinic Acid Ameliorates Deltamethrin Induced Hepatotoxicity and Nephrotoxicity(2023) Yildizhan, Eda; Ülger, Burak Veli; Akkuş, Murat; Katanalp, Ömer Faruk; Özkorkmaz, Ebru Gökalp; Yıldızhan, İbrahimAim: Deltamethrin (DM) is an insecticide and is widely used around the world. Rosmarinic acid (RA) is found in herbs and spices in the Lamiaceae (mint) family and has antioxidant, antiinflammatory and antiapoptotic effects. We objective to examine the protecting efficacy of Rosmarinic acid in preventing the toxic effects of Deltamethrin. Material and Methods: In ours study we used 28 male rats. Group 1: Control group, Group 2: RA group, RA was given 20 mg/kg peroral (p.o.) for 7 days, Group 3: DM group, 35 mg/kg/dose of DM was given 24 hours before sacrification as a single dose by gavage, Group 4: RA+ DM group. Results: BUN, creatinine, AST and ALT values of the RA+DM group were lower than the DM group. TAS and TOS grades were higher in the DM group matched to the RA+DM group. The damage scores of the DM group were higher aacording as those of the RA+DM group. Conclusion: RA has been shown to have predicative influence in the therapy of deltamethrin-induced nephrotoxicity and hepatotoxicity.Öğe Wound healing activity of Salvia huberi ethanolic extract in streptozocin-induced diabetic rats(MA Healthcare Ltd, 2023) Özay, Yusuf; Özkorkmaz, Ebru Gökalp; Kulualp, Meltem Kumaş; Kara, Sevda Güzel; Yıldırım, Zuhal; Uzun, Coşar; Çelik, AylaObjective: The aim of this study was to examine the in vivo wound healing potential of Salvia huberi Hedge (endemic to Turkey) on excision and incision wound models in diabetic rats. Method: Male Wistar albino rats, 3-4 months old and weighing 180-240g were used. The animals were randomly divided into five groups including Control, Vehicle and Fito reference, and two different concentrations (0.5% and 1% weight/weight (w/w)) of ethanol extract of Salvia huberi were investigated in both wound models on streptozocin-induced diabetic rats using macroscopic, biomechanical, biochemical, histopathological, genotoxic and gene expression methods over both seven and 14 days. Fito cream (Tripharma Drug Industry and Trade Inc., Turkey) was used as the reference drug. Results: A total of 60 rats were used in this study. Salvia huberi ointments at 0.5% and 1% (w/w) concentrations and Fito cream showed 99.3%, 99.4% and 99.1% contraction for excision wounds, and 99.9%, 97.0% and 99% contraction for incision wounds, respectively. In Salvia huberi ointments and Fito cream groups, re-epithelialisation increased dramatically by both day 7 and day 14 (p<0.05). By day 14, low hydroxyproline and malondialdehyde (MDA) levels, and high glutathione (GSH) levels were observed in the Salvia huberi ointment groups. After two application periods, damaged cell percent and genetic damage index values and micronucleus frequency of Salvia huberi ointment treatment groups were lower than Control and Vehicle groups (p<0.001). A growth factor expression reached a high level by day 7 in the Control group; in Salvia huberi-treated groups it was decreased. Conclusion: The study showed that application of Salvia huberi ointments ameliorated the healing process in diabetic rats with excisional and incisional wounds and may serve as a potent healing agent.Öğe Yardımcı Üreme Tekniklerinde Yapay Zeka(DNT Ortadoğu Yayıncılık A.Ş., 2024) Sahin, Firat; Özkorkmaz, Ebru Gökalp; Kaya, Seval; Aşır, FıratYapay zeka (YZ), son yıllarda biyomedikal alanlarda, özellikle de yardımcı üreme teknikleri (YÜT) içinde önemli bir yer edinmiştir. YÜT, infertilite tedavisinde kullanılan yöntemleri kapsar ve süreçlerin optimize edilmesi için YZ' nin entegrasyonu büyük bir potansiyele sahiptir. YZ kullanımı, sperm analizi, oosit kalitesinin değerlendirilmesi ve embriyo seçimi gibi kritik aşamalarda önemli iyileştirmeler sağlamaktadır. Ayrıca, bu süreçlerin daha hassas ve doğru bir şekilde yönetilmesine olanak tanırken, kişiselleştirilmiş tedavi yaklaşımlarının uygulanmasını da kolaylaştırır. YZ destekli sistemler, infertilite tedavisinde başarı oranlarını artırabilir, maliyetleri düşürebilir ve klinik sonuçları iyileştirebilir. YÜT alanında YZ' nin entegrasyonunun, gelecekte daha verimli ve etkili tedavi süreçlerinin geliştirilmesine katkı sağlayacağı öngörülmektedir.
