High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra
dc.contributor.author | Oluk E.A. | |
dc.contributor.author | Orhan S. | |
dc.contributor.author | Karakaş O. | |
dc.contributor.author | Çakir A. | |
dc.contributor.author | Gönüz A. | |
dc.date.accessioned | 2024-04-24T18:44:17Z | |
dc.date.available | 2024-04-24T18:44:17Z | |
dc.date.issued | 2010 | |
dc.department | Dicle Üniversitesi | en_US |
dc.description.abstract | A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 ?g/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand: peat: perlite (1:1:1; v/v/v) mixture. © 2010 Academic Journals. | en_US |
dc.identifier.endpage | 2233 | en_US |
dc.identifier.issn | 1684-5315 | |
dc.identifier.issue | 15 | en_US |
dc.identifier.scopus | 2-s2.0-77951223288 | |
dc.identifier.scopusquality | N/A | |
dc.identifier.startpage | 2229 | en_US |
dc.identifier.uri | https://hdl.handle.net/11468/24637 | |
dc.identifier.volume | 9 | en_US |
dc.indekslendigikaynak | Scopus | |
dc.language.iso | en | en_US |
dc.publisher | Academic Journals | en_US |
dc.relation.ispartof | African Journal of Biotechnology | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Hormone-Free Ms | en_US |
dc.subject | Hypericum Triquetrifolium | en_US |
dc.subject | Indirect Regeneration | en_US |
dc.title | High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra | en_US |
dc.title | High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra | |
dc.type | Article | en_US |