High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra

dc.contributor.authorOluk E.A.
dc.contributor.authorOrhan S.
dc.contributor.authorKarakaş O.
dc.contributor.authorÇakir A.
dc.contributor.authorGönüz A.
dc.date.accessioned2024-04-24T18:44:17Z
dc.date.available2024-04-24T18:44:17Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractA method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 ?g/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand: peat: perlite (1:1:1; v/v/v) mixture. © 2010 Academic Journals.en_US
dc.identifier.endpage2233en_US
dc.identifier.issn1684-5315
dc.identifier.issue15en_US
dc.identifier.scopus2-s2.0-77951223288
dc.identifier.scopusqualityN/A
dc.identifier.startpage2229en_US
dc.identifier.urihttps://hdl.handle.net/11468/24637
dc.identifier.volume9en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHormone-Free Msen_US
dc.subjectHypericum Triquetrifoliumen_US
dc.subjectIndirect Regenerationen_US
dc.titleHigh efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turraen_US
dc.titleHigh efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra
dc.typeArticleen_US

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