High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra

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Tarih

2010

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Academic Journals

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 ?g/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand: peat: perlite (1:1:1; v/v/v) mixture. © 2010 Academic Journals.

Açıklama

Anahtar Kelimeler

Hormone-Free Ms, Hypericum Triquetrifolium, Indirect Regeneration

Kaynak

African Journal of Biotechnology

WoS Q Değeri

Scopus Q Değeri

N/A

Cilt

9

Sayı

15

Künye