In Vitro Conservation and Cryopreservation of Turkish Pistachio Germplasm

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dc.contributor.authorOzden-Tokatli, Y.
dc.contributor.authorAkdemir, H.
dc.contributor.authorKaya, E.
dc.contributor.authorTilkat, E.
dc.contributor.authorYildirim, H.
dc.contributor.authorOnay, A.
dc.date.accessioned2024-04-24T17:37:32Z
dc.date.available2024-04-24T17:37:32Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description5th International Symposium on Pistachios and Almonds -- OCT 06-10, 2009 -- Univ Harran, Sanliurfa, TURKEYen_US
dc.description.abstractThe most important biotechnological methods that can be applied as a complementary strategy to safeguard pistachio germplasm comprise the use of plant tissue culture systems (organogenesis and somatic embryogenesis) and in vitro conservation strategies which include both slow growth storage and cryopreservation (storage of cells and tissues at ultra low temperatures using liquid nitrogen) techniques. As regards slow growth storage, studies were initially conducted to develop appropriate medium-term conservation techniques with maintaining in vitro cultures of mature Atli and Siirt cultivars on standard culture medium at 4 degrees C in dark for 3, 6, 9 and 12 months in the present study. Moreover, cryopreservation of seeds of Siirt, Kirmizi, and wild type of P. vera L. was also carried out by using dehydration and one-step freezing technique. Our results showed that it was possible to conserve microshoots of pistachio up to 12 months at 4 degrees C without severe loss of viability and regeneration frequency of cryopreserved seeds of pistachio was 26 - 78% germination. As the applied cryogenic technique does not require any specialised equipment, the developed protocol faciliated especially the handling and maintaining of pistachio seeds while ensuring the preservation of pistachio germplasm ad infinitum.en_US
dc.description.sponsorshipInt Soc Hort Sci (ISHS),Int Soc Food, Agr & Environm (ISFAE)en_US
dc.identifier.endpage552en_US
dc.identifier.isbn978-90-66056-34-3
dc.identifier.issn0567-7572
dc.identifier.scopus2-s2.0-84855644181
dc.identifier.scopusqualityQ4
dc.identifier.startpage545en_US
dc.identifier.urihttps://hdl.handle.net/11468/20998
dc.identifier.volume912en_US
dc.identifier.wosWOS:000313835600082
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherInt Soc Horticultural Scienceen_US
dc.relation.ispartofV International Symposium on Pistachios and Almonds
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPistachioen_US
dc.subjectSeed Cryopreservationen_US
dc.subjectSlow Growth Storageen_US
dc.titleIn Vitro Conservation and Cryopreservation of Turkish Pistachio Germplasmen_US
dc.titleIn Vitro Conservation and Cryopreservation of Turkish Pistachio Germplasm
dc.typeConference Objecten_US

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