Phytochemical profile by LC-MS/MS analysis and evaluation of antioxidant, antidiabetic, anti-Alzheimer, and anticancer activity of Onobrychis argyrea leaf extracts

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dc.contributor.authorYeniceri, Esma
dc.contributor.authorAltay, Ahmet
dc.contributor.authorKoksal, Ekrem
dc.contributor.authorAltin, Sevgi
dc.contributor.authorTaslimi, Parham
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorCakir, Oguz
dc.date.accessioned2024-04-24T16:11:27Z
dc.date.available2024-04-24T16:11:27Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractIntroduction: Studies on different species of Onobrychis, a member of the Fabaceae family, have revealed a number of biological activities with potential applications in alternative medicine. The phytochemical content of the plant Onobrychis argyrea subsp. argyrea and some of the important biological activities linked to its metabolism are quite limited. Therefore, this study was the first to determine the content of secondary metabolites in this species and to elucidate important biological activities related to metabolism. Methods: LC-MS/MS was used for the quantitative analysis of bioactive compounds in the aerial part of O. argyrea extracts. The antioxidant properties of the extracts were assessed based on their ability to scavenge DPPH radicals and reduce iron ions. The anti-diabetic effect of the extracts was tested by the inhibition of alpha-amylase and alpha-glycosidase enzymes, and the anti-Alzheimer's ability was tested by the inhibition of the enzymes AChE and BChE. The cytotoxic effects of the extracts were tested on four different cancer lines, namely A-549, HT-29, MCF-7 and MDA-MB 453 using the XTT assay. The mechanism of the anti-cancer effect was determined by means of flow cytometry analysis on HT-29 cells. Results: Twenty-nine phytochemicals were identified via the LC-MS/MS analysis in the plant extracts. The most abundant phytochemicals in the extracts were quinic acid, isoquercitrin, epicatechin, and routine, respectively. Antioxidant analysis showed that among all extracts, methanol extract (ME) was most effective in scavenging DPPH (IC50: 23.93 +/- 0.96 mu g/mL) and in reducing iron ions (53.24 +/- 2.14 mgTEAC/g). Enzyme activity studies demonstrated that ME is a potent inhibitor of alpha-glycosidase (IC50: 15.06 +/- 0.64 mu g/mL), AChE (IC50: 24.75 +/- 0.76 mu g/mL), and BChE (IC50: 3.36 +/- 0.14 mu g/mL). XTT assay results disclosed the strongest anti-proliferative activity of ME against the human colorectal adenocarcinoma HT-29 cell line with an IC50 value of 33.35 +/- 0.92 mu g/mL. Flow cytometry analysis revealed that ME caused mitochondrial membrane damage in HT-29 cells (**p<0.01) and subsequently induced apoptosis (**p<0.01) by activating the caspase pathway (**p<0.001). Conclusion: O. argyrea, rich in bioactive compounds, showed remarkable antioxidant, antidiabetic, Alzheimer's, and anticancer properties in vitro, demonstrating that this plant contains secondary metabolites that may be potential drug candidates for the treatment of metabolic diseases.en_US
dc.description.sponsorshipErzincan Uni-versity Scientific Research Projects Coordination Commission (EU-BAP) [2022-842]en_US
dc.description.sponsorshipThis work was financially supported by grants from Erzincan Uni-versity Scientific Research Projects Coordination Commission (EU-BAP) (Project No: 2022-842) .en_US
dc.identifier.doi10.1016/j.eujim.2024.102337
dc.identifier.issn1876-3820
dc.identifier.issn1876-3839
dc.identifier.scopus2-s2.0-85183387691
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.eujim.2024.102337
dc.identifier.urihttps://hdl.handle.net/11468/15398
dc.identifier.volume66en_US
dc.identifier.wosWOS:001182276700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofEuropean Journal of Integrative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOnobrychis Argyreaen_US
dc.subjectAntioxidant Activityen_US
dc.subjectEnzyme Inhibitionen_US
dc.subjectCytotoxicityen_US
dc.subjectApoptosisen_US
dc.subjectLc-Ms/Msen_US
dc.titlePhytochemical profile by LC-MS/MS analysis and evaluation of antioxidant, antidiabetic, anti-Alzheimer, and anticancer activity of Onobrychis argyrea leaf extractsen_US
dc.titlePhytochemical profile by LC-MS/MS analysis and evaluation of antioxidant, antidiabetic, anti-Alzheimer, and anticancer activity of Onobrychis argyrea leaf extracts
dc.typeArticleen_US

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