Molecularly imprinted affinity cryogels for the selective recognition of myoglobin in blood serum

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dc.contributor.authorDolak, Ibrahim
dc.contributor.authorKecili, Rustem
dc.contributor.authorOnat, Ruken
dc.contributor.authorZiyadanogullari, Berrin
dc.contributor.authorErsoz, Arzu
dc.contributor.authorSay, Ridvan
dc.date.accessioned2024-04-24T16:15:36Z
dc.date.available2024-04-24T16:15:36Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description3rd International Turkish Congress on Molecular Spectroscopy (TURCMOS) -- AUG 26-29, 2017 -- Bodrum, TURKEYen_US
dc.description.abstractThe present work demonstrates the selective recognition of myoglobin in human serum in the presence of various competitive proteins such as lysozyme, cytochrome c and hemoglobin by using molecularly imprinted affinity cryogels. The imprinted affinity cryogels towards myoglobin were prepared by using cryopolymerization technique. For this purpose, N-methacryloylamido antipyrine (MAAP)-Ce(III) and myoglobin were used as the complex functional monomer and template molecule, respectively. (MAAP)-Ce(III) complex functional in the imprinted cryogel provided a luminescence feature to the cryogel and the detection of the myoglobin was successfully achieved by UV-VIS-Near Infrared (UV-VIS-NIR) Spectroscopy. The effects of some variables such as initial myoglobin concentration, temperature, pH and ionic strength on myoglobin binding to the prepared cryogels were studied. The myoglobin binding capacity of the prepared cryogels was determined to be 68 mgg(-1). Myoglobin binding to the cryogels in the presence of competitive proteins lysozyme, cytochrome c and hemoglobin were also performed. The imprinted cryogels exhibited high selectivity towards myoglobin and the obtained binding order under competitive conditions was myoglobin > lysozyme > cytochrome c > hemoglobin. (C) 2018 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.molstruc.2018.03.126
dc.identifier.endpage176en_US
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.scopus2-s2.0-85053034414en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage171en_US
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2018.03.126
dc.identifier.urihttps://hdl.handle.net/11468/15867
dc.identifier.volume1174en_US
dc.identifier.wosWOS:000449132500025en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMolecularly Imprinted Cryogelsen_US
dc.subjectMyoglobinen_US
dc.subjectSelective Recognitionen_US
dc.subjectAcute Myocardial Infarctionen_US
dc.titleMolecularly imprinted affinity cryogels for the selective recognition of myoglobin in blood serumen_US
dc.typeConference Objecten_US

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