DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cyclotrichium niveum

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dc.contributor.authorEmen, Sevil
dc.contributor.authorCeken, Bircan
dc.contributor.authorKizil, Goksel
dc.contributor.authorKizil, Murat
dc.date.accessioned2024-04-24T17:07:46Z
dc.date.available2024-04-24T17:07:46Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractCyclotrichium niveum (Boiss.) Manden. & Scheng., a herb of the Labiate family, has been widely used as a flavoring agent in Turkey; it was examined for its antioxidant potential and DNA damage protecting activity. The antioxidant activity of C niveum aerial parts (stems, leaves, and flowers) was determined from the methanol extract. The antioxidant potency of methanol extract of C niveum was investigated employing various established in vitro systems, such as lipid peroxidation in rat brain homogenate, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hyrdoxyl radical scavenging, reducing power, iron chelation, and inhibitory effect on protein oxidation. The methanol extract of C niveum was able to reduce the stable free radical DPPH with an IC50 of 78.15 mu g/mL. The metal chelating ability was found to be low compare with EDTA. Methanol extract of C. niveum aerial parts showed strong inhibitory activity toward lipid peroxidation of rat brain homogenate induced by the FeCl2-H2O2 system. Furthermore, C. niveum extract exhibited a strong concentration-dependent inhibition of deoxyribose oxidation. The effect on DNA cleavage induced by H2O2 UV-photolysis was also investigated. It showed a concentration-dependent free radical scavenging capacity and protective effect on DNA cleavage. In addition, the oxidative damage of bovine serum albumin (BSA), induced by hydroxyl radical in an acellular system, was inhibited by 10-1000 mu g/mL of methanol extract of C. niveum. The total phenolic content of C niveum was detected in methanol as 200.9 mu g gallic acid/mg extract. These results clearly demonstrated that C niveum extract possesses a marked antioxidant activity and it is a potential source of natural antioxidants.en_US
dc.description.sponsorshipDicle University Research Committee [02-FF-10, 03-FF-63]en_US
dc.description.sponsorshiphe authors would like to thank Professor Selcuk Ertekin for identification of the plant sample and Assistant Professor Zeki Kanay for his help with this work. This work was supported by research grants from the Dicle University Research Committee (DUBAP, Project nos.: 02-FF-10 and 03-FF-63).en_US
dc.identifier.doi10.1080/13880200802435903
dc.identifier.endpage229en_US
dc.identifier.issn1388-0209
dc.identifier.issn1744-5116
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-77149138691
dc.identifier.scopusqualityQ1
dc.identifier.startpage219en_US
dc.identifier.urihttps://doi.org/10.1080/13880200802435903
dc.identifier.urihttps://hdl.handle.net/11468/16974
dc.identifier.volume47en_US
dc.identifier.wosWOS:000268221400006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofPharmaceutical Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCyclotrichium Niveumen_US
dc.subjectAntioxidant Activityen_US
dc.subjectDna Damageen_US
dc.subjectRadical Scavenging Abilityen_US
dc.subjectLipid Peroxidationen_US
dc.subjectProtein Oxidationen_US
dc.titleDNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cyclotrichium niveumen_US
dc.titleDNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cyclotrichium niveum
dc.typeArticleen_US

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