Preparation and characterization of surface modified ?-Fe2O3 (maghemite)-silica nanocomposites used for the purification of benzaldehyde lyase

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dc.contributor.authorTural, Bilsen
dc.contributor.authorSopaci, S. Betul
dc.contributor.authorOzkan, Necati
dc.contributor.authorDemir, Ayhan S.
dc.contributor.authorVolkan, Murvet
dc.date.accessioned2024-04-24T16:15:17Z
dc.date.available2024-04-24T16:15:17Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractgamma-Fe2O3 (maghemite)-silica nanocomposite particles were synthesized using a sol-gel method. The condensation products of 3-glycidoxy propyltrimethoxy silane (GPTMS) and nitrilotriacetic acid (NTA) were introduced onto the surfaces of the gamma-Fe2O3 silica nanocomposite particles and subsequently, these modified surfaces were complexed with cobalt (Co+2) metal ions. A possibility of using these surface modified gamma-Fe2O3-silica particles for the purification of 6 x histidine tagged recombinant benzaldehyde lyase (BAL, EC 4.1.2.38) based on magnetic separation was investigated. X-ray diffraction (XRD), thermal analysis, and vibrating sample magnetometry (VSM) methods were used to characterize the surface modified superparamagnetic gamma-Fe2O3 (maghemite)-silica nanoparticles. XRD (Scherer's equation) results indicate that the primary particle size of maghemite was around 11 nm. Magnetic characterization results confirmed that the gamma-Fe2O3 (maghemite)-silica nanoparticles were superparamagnetic. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results, these superparamagnetic nanoparticles specifically capture 6 x His-tagged BAL from crude extract of Escherichia coli (E. coli) BL21(DE3)pLysS/BAL(HIS). This study shows that the surface modified gamma-Fe2O3 (maghemite)-silica nanoparticles are eligible for immobilized metal-ion affinity adsorption for histidine tagged recombinant proteins with its high capacity (3.16 +/- 0.4 mg/g) and selectivity. (C) 2011 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.jpcs.2011.05.010
dc.identifier.endpage973en_US
dc.identifier.issn0022-3697
dc.identifier.issn1879-2553
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-79959556976
dc.identifier.scopusqualityQ1
dc.identifier.startpage968en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpcs.2011.05.010
dc.identifier.urihttps://hdl.handle.net/11468/15740
dc.identifier.volume72en_US
dc.identifier.wosWOS:000292665700010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofJournal of Physics and Chemistry of Solids
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMagnetic Materialsen_US
dc.subjectNanostructuresen_US
dc.subjectSurfacesen_US
dc.subjectX-Ray Diffractionen_US
dc.subjectMagnetic Propertiesen_US
dc.titlePreparation and characterization of surface modified ?-Fe2O3 (maghemite)-silica nanocomposites used for the purification of benzaldehyde lyaseen_US
dc.titlePreparation and characterization of surface modified ?-Fe2O3 (maghemite)-silica nanocomposites used for the purification of benzaldehyde lyase
dc.typeArticleen_US

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