Pumice particle interface: a case study for immunoglobulin G purification

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dc.contributor.authorAlacabey, Ihsan
dc.contributor.authorAcet, Omur
dc.contributor.authorOnal, Burcu
dc.contributor.authorDikici, Emrah
dc.contributor.authorKarakoc, Veyis
dc.contributor.authorGurbuz, Fatma
dc.contributor.authorAlkan, Huseyin
dc.date.accessioned2024-04-24T16:00:03Z
dc.date.available2024-04-24T16:00:03Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractCryogels with embedded natural adsorbent are new trend of chromatographic media for separation of biomolecules. In this report, experimental determination of immunoglobulin G (IgG) purification by Cu2+-attached pumice particles unified cryogel (Cu2+-PPUC) was performed. For this purpose, after preparation of Cu2+-attached pumice particles, they were unified with 2-hydroxyethyl methacrylate monomers to produce Cu2+-PPUC through polymerization of gel-forming precursors at subzero temperatures. IgG separation experiments were accomplished in a continuous column system. The highest binding capacity (596.8 mg/g) was obtained by working with 0.02 M phosphate buffer at pH 6.0. The chemical analysis of pumice was examined by X-ray fluorescence spectrometer. Scanning electron microscopy was performed to identify the morphology of Cu2+-PPUC. Langmuir adsorption model was best fitted to interaction when compared to Freundlich model. Temkin model was utilized to characterize adsorption, energetically. Purification ability of Cu2+-PPUC for IgG was shown with high selectivity via reducing SDS-PAGE electrophoresis.en_US
dc.identifier.doi10.1007/s00289-020-03392-0
dc.identifier.endpage5607en_US
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-85092112891
dc.identifier.scopusqualityQ1
dc.identifier.startpage5593en_US
dc.identifier.urihttps://doi.org/10.1007/s00289-020-03392-0
dc.identifier.urihttps://hdl.handle.net/11468/14356
dc.identifier.volume78en_US
dc.identifier.wosWOS:000575725000003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectComposite Cryogelsen_US
dc.subjectPumice Particlesen_US
dc.subjectImacen_US
dc.subjectIgg Separationen_US
dc.titlePumice particle interface: a case study for immunoglobulin G purificationen_US
dc.titlePumice particle interface: a case study for immunoglobulin G purification
dc.typeArticleen_US

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