Fabrication of an electrochemical E. coli biosensor in biowells using bimetallic nanoparticle-labelled antibodies

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dc.contributor.authorEkşi, Haslet
dc.contributor.authorGüzel, Remziye
dc.contributor.authorGüven, Burcu
dc.contributor.authorBoyacı, İsmail Hakkı
dc.contributor.authorSolak, Ali Osman
dc.contributor.orcid0000-0003-1333-060X
dc.date.accessioned2024-04-24T15:59:23Z
dc.date.available2024-04-24T15:59:23Z
dc.date.issued2015
dc.departmentDicle Üniversitesi, Ziya Gökalp Eğitim Fakültesi, Matematik ve Fen Bilimleri Eğitimi Bölümüen_US
dc.description.abstractAn electrochemical biosensor was developed for the determination of Escherichia coli (E. coli) in water. For this purpose, silver-gold core-shell (Ag@Au) bioconjugates and anti-E. coli modified PS-microwells were designed in a sandwich-type format in order to obtain higher sensitivity and selectivity. Ag@Au bimetallic nanoparticles were synthesized by co-reduction method. The core-shell formation was analyzed by using UV-Vis spectroscopy and transmission electron microscopy. Biotin labeled anti-E. coli antibodies were coupled with Ag@Au nanoparticles to form bioconjugates. The electrochemical immunosensor was prepared by immobilizing anti-E. coli on polystyrene (PS)-microwells via chemical bonding. These modified microwells were identified with X-ray photoelectron spectroscopy and surface enhanced Raman spectroscopy. E. coli was sandwiched between Ag@Au bioconjugates and anti-E. coli on PS-microwells at different concentrations. The relationship between the E. coli concentration and stripping current of gold ions (Au3+) were investigated by square wave anodic stripping voltammetry at pencil graphite electrode. The proposed method can provide some advantages such as lower detection limit and shorter detection time. The electrochemical response for the immunosensor was linear with the concentration of the E. coli in the range of 10(1) and 10(5) cfu/mL with a limit of detection 3 cfu/mL. The procedure maintains good sensitivity and repeatability and also offers utility in the fields of environmental monitoring and clinical diagnosis.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [109T633]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey, Project Number: 109T633.en_US
dc.identifier.citationEkşi, H., Güzel, R., Güven, B., Boyacı, İ. H. ve Solak, A. O. (2015). Fabrication of an electrochemical E. coli biosensor in biowells using bimetallic nanoparticle-labelled antibodies. Electroanalysis, 27(2), 343-352.
dc.identifier.doi10.1002/elan.201400370
dc.identifier.endpage352en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84931054258
dc.identifier.scopusqualityQ2
dc.identifier.startpage343en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201400370
dc.identifier.urihttps://hdl.handle.net/11468/14032
dc.identifier.urihttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elan.201400370
dc.identifier.volume27en_US
dc.identifier.wosWOS:000349558900011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorGüzel, Remziye
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBimetallic nanoparticlesen_US
dc.subjectE. Colien_US
dc.subjectAnodic stripping voltammetryen_US
dc.subjectPencil graphite electrodeen_US
dc.titleFabrication of an electrochemical E. coli biosensor in biowells using bimetallic nanoparticle-labelled antibodiesen_US
dc.titleFabrication of an electrochemical E. coli biosensor in biowells using bimetallic nanoparticle-labelled antibodies
dc.typeArticleen_US

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