Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L.

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dc.contributor.authorTilkat, Engin
dc.contributor.authorOnay, Ahmet
dc.contributor.authorYildirim, Hakan
dc.contributor.authorAyaz, Emine
dc.date.accessioned2024-04-24T16:18:22Z
dc.date.available2024-04-24T16:18:22Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractA protocol was developed for direct shoot and plantlet regeneration from in vitro regenerated leaf explants of male Pistacia vera L. cv. 'Atli'. Leaves excised from axenic shoot cultures of pistachio were used to induce organogenesis on a Murashige and Skoog (MS) medium with Gamborg vitamins supplemented with combinations of different concentrations of BAP and IAA. The highest adventitious shoot regeneration in 35% of the explants, with the number of shoots ranging from 2 to 3 per explant, occurred in the explants cultured during the establishment phase in the medium with 1 mg l(-1) IAA and 2 mg l(-1) BAR For shoot multiplication, the highest number of new microshoot/explants (5.76) was obtained in a culture medium supplemented with 1 mg l(-1) BAP, but it was not significantly different from the number obtained at 2 mg l(-1) BAP. A high rooting frequency (84%) for nicroshoots was recorded on a medium supplemented with 2 mg l(-1) IBA. In vitro rooted plantlets were transferred to pots filled with a mixture of soil, sand and peat (1: 1: 1). They were weaned in a growth room and finally moved to a greenhouse. This protocol could be utilized for in vitro clonal propagation of this economically important plant. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK [TOVAG-3355]; DUBAP-Dicle University Research Project Council [(DUBAP)-05-FF-23, 05-FF-61]en_US
dc.description.sponsorshipThe study was funded by grant #TOVAG-3355 from TUBITAK-The Scientific and Technical Research Council of Turkey. The study was partially funded by a grant from DUBAP-Dicle University Research Project Council (DUBAP)-05-FF-23 and 05-FF-61. We also thank Linda Thain Ali for linguistically reviewing the manuscript.en_US
dc.identifier.doi10.1016/j.scienta.2009.02.007
dc.identifier.endpage365en_US
dc.identifier.issn0304-4238
dc.identifier.issn1879-1018
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-67349246588
dc.identifier.scopusqualityQ1
dc.identifier.startpage361en_US
dc.identifier.urihttps://doi.org/10.1016/j.scienta.2009.02.007
dc.identifier.urihttps://hdl.handle.net/11468/16037
dc.identifier.volume121en_US
dc.identifier.wosWOS:000266762000018
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofScientia Horticulturae
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeaf Culturesen_US
dc.subjectMale Treesen_US
dc.subjectMicropropagationen_US
dc.subjectPistachioen_US
dc.subjectOrganogenesisen_US
dc.titleDirect plant regeneration from mature leaf explants of pistachio, Pistacia vera L.en_US
dc.titleDirect plant regeneration from mature leaf explants of pistachio, Pistacia vera L.
dc.typeArticleen_US

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