Purification of glucose 6-phosphate dehydrogenase from goose erythrocytes and kinetic properties

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dc.contributor.authorBeydemir, S
dc.contributor.authorYilmaz, H
dc.contributor.authorÇiftçi, M
dc.contributor.authorBakan, E
dc.contributor.authorKüfrevioglu, ÖI
dc.date.accessioned2024-04-24T17:37:53Z
dc.date.available2024-04-24T17:37:53Z
dc.date.issued2003
dc.departmentDicle Üniversitesien_US
dc.description.abstractGlucose 6-phosphate dehydrogenase (G6PD) was purified from goose erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of 3 steps: hemolysate preparation, ammonium sulfate precipitation, and 2', 5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 3 consecutive procedures, the enzyme, having a specific activity of 36.2 EU/mg protein, was purified for a yield of 68.79% and 3892 folds; to ascertain enzyme purity, SDS-PAGE was performed. Optimal pH, stable pH, optimal temperature, molecular weight, and K-m and V-max values for NADP(+) and glucose 5-phosphate (G6-P) substrates were also determined for the enzyme. In addition, K-i values and inhibition type were determined by means of Lineweaver-Burk graphs obtained for such inhibitors as ATP, ADP and NADPH. These materials inhibited the enzyme in a noncompetitive manner.en_US
dc.identifier.endpage1185en_US
dc.identifier.issn1300-0128
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-0242521479
dc.identifier.scopusqualityQ3
dc.identifier.startpage1179en_US
dc.identifier.urihttps://hdl.handle.net/11468/21230
dc.identifier.volume27en_US
dc.identifier.wosWOS:000186808800020
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGooseen_US
dc.subjectPurificationen_US
dc.subjectGlucose 6-Phosphate Dehydrogenaseen_US
dc.subjectErythrocyteen_US
dc.titlePurification of glucose 6-phosphate dehydrogenase from goose erythrocytes and kinetic propertiesen_US
dc.titlePurification of glucose 6-phosphate dehydrogenase from goose erythrocytes and kinetic properties
dc.typeArticleen_US

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