Astragalus diphtherites FENZL var. diphtherites ve astragalus gymnalopecias RECH. FIL’in gövde ve kök kısımlarından farklı çözücüler ile elde edilen özütlerin invitro antioksidan ve antimikrobiyal özelliklerinin belirlenmesi
Yükleniyor...
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kahramanmaraş Sütçü İmam Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı farkı polariteye sahip çözücü serilerinden
geçiren Astragalus diphtherites var. diphtherites (A. diphtherites)
ve Astragalus gymnalopecias (A. gymnalopecias) türlerinin gövde ve
kök kısımlarının antioksidan ve antimikrobiyal özelliklerinin
araştırılmasıdır. Antioksidan özellikleri belirlemek için özütlerin
total fenolik, total flavonoid, DPPH, metal şelatlama, indirgeme
gücü ve hidroksi radikalini söndürme aktiviteleri test edildi. En
yüksek total fenolik bileşen miktarı A. diphtherites’ de gövdede
metanol, kökte ise aseton özütünden elde edilirken A.
gymnalopecias’ ta gövdede metanol kökte ise etil asetat özütünde
tespit edildi. A. diphtherites ve A. gymnalopecias’ ta en yüksek total
flavonoid miktarları ve indirgeme gücü aktiviteleri gövdede aseton
kökte ise etil asetat özütlerinden elde edildi. A. diphtherites için en
yüksek DPPH radikalini söndürme aktivitesi, gövdede metanol
kökte ise aseton özütünde, A. gymnalopecias’ ta gövdede metanol
kökte ise etil asetat özütünde tespit edildi. A. diphtherites’ te gövde
kısmından elde edilen metanol ve kök kısmından elde edilen hekzan
özütleri, A. gymnalopecias’ ta ise gövde ve kök kısımlarının metanol
özütleri en yüksek metal şelatlama aktivitesi gösterdi. Her iki türde
de gövde ve kök etil asetat özütleri en yüksek hidroksil radikali
söndürme aktivitesi gösterdi. A. gymnalopecias’ın gövde aseton ve
metanol özütlerinin Streptococcus pyogenes’in büyümesi üzerinde
inhibisyon etkisine sahip olduğu tespit edildi.
Objective of this study was to investigate the antioxidant and antimicrobial properties of shoot and root parts of Astragalus diphtherites var. diphtherites (A. diphtherites) and Astragalus gymnalopecias (A. gymnalopecias) species which were subjected to solvent series with different polarity. Total phenolic, total flavonoid, 2.2-diphenyl-1-picryl-hydrazyl (DPPH), metal chelating, reducing power and hydroxy radicals scavenging activities of the extracts were tested to determine antioxidant properties. The highest total phenolic content of A. diphtherites, was obtained from the methanol extract of shoots and the acetone extract of root parts. On the other hand, the highest total phenolic content in A. gymnalopecias were achieved from methanol extract of shoot parts and the ethyl acetate extract of the root part. The highest amount of total flavonoids and reducing power activities of A. diphtherites and A. gymnalopecias were obtained from the acetone extracts in the shoot part and from the ethyl acetate extracts in the root part. While the highest DPPH radical scavenging activity was determined in the methanol extract in the shoot part and the acetone extract in the root part of A. diphtherites. The highest DPPH radical scavenging activity for A. gymnalopecias was determined in the methanol extract of the shoot part and the ethyl acetate extract of the root part. The highest metal chelating activity was seen in the methanol extracts from shoot parts and in the hexane extracts from the root part of A. diphtherites. The ethyl acetate extracts of the shoot and root part in both species showed the highest hydroxyl radical scavenging activity. It was determined that acetone and methanol extracts of the shoot part of A. gymnalopecias have inhibition effect on the growth of Streptococcus pyogenes
Objective of this study was to investigate the antioxidant and antimicrobial properties of shoot and root parts of Astragalus diphtherites var. diphtherites (A. diphtherites) and Astragalus gymnalopecias (A. gymnalopecias) species which were subjected to solvent series with different polarity. Total phenolic, total flavonoid, 2.2-diphenyl-1-picryl-hydrazyl (DPPH), metal chelating, reducing power and hydroxy radicals scavenging activities of the extracts were tested to determine antioxidant properties. The highest total phenolic content of A. diphtherites, was obtained from the methanol extract of shoots and the acetone extract of root parts. On the other hand, the highest total phenolic content in A. gymnalopecias were achieved from methanol extract of shoot parts and the ethyl acetate extract of the root part. The highest amount of total flavonoids and reducing power activities of A. diphtherites and A. gymnalopecias were obtained from the acetone extracts in the shoot part and from the ethyl acetate extracts in the root part. While the highest DPPH radical scavenging activity was determined in the methanol extract in the shoot part and the acetone extract in the root part of A. diphtherites. The highest DPPH radical scavenging activity for A. gymnalopecias was determined in the methanol extract of the shoot part and the ethyl acetate extract of the root part. The highest metal chelating activity was seen in the methanol extracts from shoot parts and in the hexane extracts from the root part of A. diphtherites. The ethyl acetate extracts of the shoot and root part in both species showed the highest hydroxyl radical scavenging activity. It was determined that acetone and methanol extracts of the shoot part of A. gymnalopecias have inhibition effect on the growth of Streptococcus pyogenes
Açıklama
Anahtar Kelimeler
Astragalus diphtherites FENZL var. diphtherites, Astragalus gymnalopecias RECH. FIL., Antioksidan, Antimikrobiyal, Biyolojik aktivite, Antioxidant, Antimicrobial, Biological activity
Kaynak
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
21
Sayı
2
Künye
Keskin, C., Özen, H. Ç., Toker, Z., Kızıl, G. ve Kızıl, M. (2018). Astragalus diphtherites FENZL var. diphtherites ve astragalus gymnalopecias RECH. FIL’in gövde ve kök kısımlarından farklı çözücüler ile elde edilen özütlerin invitro antioksidan ve antimikrobiyal özelliklerinin belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(2), 157-166.
