A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

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dc.contributor.authorAg Seleci, Didem
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorYavuz, Murat
dc.contributor.authorSeleci, Muharrem
dc.contributor.authorBongartz, Rebecca
dc.contributor.authorStahl, Frank
dc.contributor.authorCoskunol, Hakan
dc.date.accessioned2024-04-24T17:21:04Z
dc.date.available2024-04-24T17:21:04Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025-1.0 mg/mL and 1.0-100 mu g/mL concentrations for 24 h, the IC50 values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.en_US
dc.description.sponsorshipKonrad Adenauer Foundationen_US
dc.description.sponsorshipThe authors thank TABIA for providing black cumin seed oil and wheat germ oil. The Konrad Adenauer Foundation is acknowledged for financial support of PhD student Didem Ag Seleci. The authors also thank SEM (Izmir) for their support during the HPLC and GC analysis.en_US
dc.identifier.doi10.3906/kim-1502-72
dc.identifier.endpage812en_US
dc.identifier.issn1300-0527
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84938305229
dc.identifier.scopusqualityQ3
dc.identifier.startpage801en_US
dc.identifier.urihttps://doi.org/10.3906/kim-1502-72
dc.identifier.urihttps://hdl.handle.net/11468/19356
dc.identifier.volume39en_US
dc.identifier.wosWOS:000359063900008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBlack Cumin Seed Oilen_US
dc.subjectWheat Germ Oilen_US
dc.subjectCell Culture Investigationsen_US
dc.titleA case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oilen_US
dc.titleA case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil
dc.typeArticleen_US

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