Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets

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dc.contributor.authorKoc, Ibrahim
dc.contributor.authorAkdemir, Hulya
dc.contributor.authorOnay, Ahmet
dc.contributor.authorCiftci, Yelda Ozden
dc.date.accessioned2024-04-24T16:02:32Z
dc.date.available2024-04-24T16:02:32Z
dc.date.issued2014
dc.departmentDicle Üniversitesien_US
dc.description.abstractGenetic stability of plants during in vitro propagation and conservation is one of the important aspects of plant biotechnology. In the present study, micropropagated P. lentiscus L. shoot cultures, which are cultivated for the mastic resin, have been cold stored up to 12 months at 4 A degrees C in the dark for different durations (2, 4, 6, 8, 10 and 12 months) and genetic alterations in cold storage conditions were evaluated. Growth parameters such as proliferation rate, shoot numbers per explant, shoot lengths and shoot forming capacity were also calculated. Since the highest proliferation rate (100 %) was obtained in 6 month-stored shoot cultures without any severe influence of cold stress on proliferation ability, amplified fragment length polymorphism (AFLP) and inter-retrotransposon amplified polymorphism (IRAP) marker systems were used to determine genetic stability in the plantlets after this storage period. Totally, 702 scorable bands were produced by 10 AFLP primer pairs. Genetic similarity value of the non-stored (control) plant and cold-stored clones ranged from 0.66 to 0.84 with a mean of 0.74. In the case of IRAP, 159 bands were produced by 8 IRAP primers. Genetic similarity value of the non-stored plant and cold-stored clones varied from 0.65 to 0.83 and the average genetic similarity value was determined as 0.72. The genetic similarity indices revealed that genetic variability was similar in both techniques. Our results showed that tissue culture and especially cold storage of P. lentiscus L. may result transposons activation, thus could cause genetic instability.en_US
dc.description.sponsorshipTUBITAK-The Scientific and Technological Research Council of Turkey [KBAG110T941]en_US
dc.description.sponsorshipThis study was supported by a grant from TUBITAK-The Scientific and Technological Research Council of Turkey (No. KBAG110T941).en_US
dc.identifier.doi10.1007/s11738-014-1610-0
dc.identifier.endpage2384en_US
dc.identifier.issn0137-5881
dc.identifier.issn1861-1664
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-84907598802
dc.identifier.scopusqualityQ1
dc.identifier.startpage2373en_US
dc.identifier.urihttps://doi.org/10.1007/s11738-014-1610-0
dc.identifier.urihttps://hdl.handle.net/11468/14827
dc.identifier.volume36en_US
dc.identifier.wosWOS:000340584400009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofActa Physiologiae Plantarum
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPistacia Lentiscus L.en_US
dc.subjectCold Stressen_US
dc.subjectRetrotransposonen_US
dc.subjectIrapen_US
dc.subjectAflpen_US
dc.titleCold-induced genetic instability in micropropagated Pistacia lentiscus L. plantletsen_US
dc.titleCold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets
dc.typeArticleen_US

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