Isolation of a thermophilic Anoxybacillus flavithermus sp nov and production of thermostable ?-amylase under solid-state fermentation (SSF)

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dc.contributor.authorOzdemir, Sadin
dc.contributor.authorMatpan, Fatma
dc.contributor.authorOkumus, Veysi
dc.contributor.authorDundar, Abdurrahman
dc.contributor.authorUlutas, Mehmet Sefa
dc.contributor.authorKumru, Mert
dc.date.accessioned2024-04-24T16:02:51Z
dc.date.available2024-04-24T16:02:51Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractA new bacteria was isolated from hot-spring water of Gazligol, Afyonkarahisar in Turkey. Based on morphological and biochemical tests and 16S rRNA gene sequence analysis, the isolate belonged to the Anoxybacillus flavithermus species which has 99% similarity with the bacterium DNA. The production of alpha-amylase by thermophilic Anoxybacillus flavithermus was investigated under solid-state fermentation by using some agricultural waste as substrates. Solid substrates such as rice husk, banana husk, millet, water melon husk, lentil bran, wheat bran and maize oil cake were studied for enzyme production. Of these, rice husk was proved as the best substrate for alpha-amylase production (1,271 U/mg). The maximum alpha-amylase production was observed as 1,803 U/mg at 72 h, 1,000 mu m particle size, 70% initial moisture content (w/v), and 40% inoculum level (v/w). Among the various nitrogen sources tested, 1% peptone (3,170 U/mg) was found to be the best nitrogen source for alpha-amylase production. As additional carbon sources, 1% starch (2,364 U/mg) enhanced alpha-amylase production. The optimum temperature for the activity of alpha-amylase was found to be 70A degrees C. The enzyme was optimally active at pH 6.0 and stable in the pH range of 6.0-8.0.en_US
dc.identifier.doi10.1007/s13213-011-0385-4
dc.identifier.endpage1375en_US
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84871428842
dc.identifier.scopusqualityQ2
dc.identifier.startpage1367en_US
dc.identifier.urihttps://doi.org/10.1007/s13213-011-0385-4
dc.identifier.urihttps://hdl.handle.net/11468/14937
dc.identifier.volume62en_US
dc.identifier.wosWOS:000310886200004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherBmcen_US
dc.relation.ispartofAnnals of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAgro-Industrialen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectSolid-State Fermentation (Ssf)en_US
dc.subjectThermophilic Bacteriaen_US
dc.titleIsolation of a thermophilic Anoxybacillus flavithermus sp nov and production of thermostable ?-amylase under solid-state fermentation (SSF)en_US
dc.titleIsolation of a thermophilic Anoxybacillus flavithermus sp nov and production of thermostable ?-amylase under solid-state fermentation (SSF)
dc.typeArticleen_US

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