Comprehensive study of chemical composition and biological activity of Thymus pubescens Boiss. et Kotschy ex Celak

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dc.contributor.authorYigitkan, Serkan
dc.contributor.authorAkdeniz, Mehmet
dc.contributor.authorYener, Ismail
dc.contributor.authorSeker, Zeki
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorFirat, Mehmet
dc.contributor.authorKavak, Deniz Evrim
dc.date.accessioned2024-04-24T16:18:20Z
dc.date.available2024-04-24T16:18:20Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe use of Thymus species amongst the public and their importance in the scientific world is increasing day by day. In addition to being consumed as tea and spice, Thymus species are used as folk medicine for respiratory, digestive, skin, circulatory, genital, nervous, visual and urinary diseases. In this study, it was aimed to examine the essential oil and ethanol extract of the root and aerial parts of Thymus pubescens Boiss. et Kotschy ex Celak in the terms of their biological activity and chemical content. The phenolic content of the species was determined by LC-MS/MS, while triterpenoid content, the chemical composition of the essential oil as well as flavour was determined by GC-MS. In addition, their antioxidant and cytotoxic activities, as well as acetyl- (AChE) and butyrylcholinesterase (BChE), urease, tyrosinase, elastase, collagenase, HMG-CoA reductase and angiotensin-converting enzyme (ACE) inhibitory activities were studied. Thymol (53.33%) was determined as the major component in the essential oil, while b-fenchyl alcohol (43.87%) was determined as the major component of the flavour. According to the LC-MS/MS and GC-MS results, it was found that especially the aerial parts of the species have a high amount of rosmarinic acid (3875.76 mg analyte/g extract), quinic acid (2392.55), naringenin (970.39), oleanolic (92785.96) and ursolic (63373.32) acids. The essential oil of T. pubescens species was observed to show high activity in four antioxidant assays, e.g. inhibition of lipid peroxidation, DPPH and ABTS radical scavenging activity as well as CUPRAC, while the ethanol extracts showed moderate antioxidant activity. In enzyme inhibition assays, the aerial parts exerted marked BChE, elastase, and collagenase inhibitory activities (92.43 +/- 1.28%, 42.59 +/- 0.56, and 48.61 +/- 0.39 at 100 mg/mL, respectively). On the other hand, AChE, urease, tyrosinase, HMG-CoA reductase and ACE inhibitory activities of all extracts were from low to moderate levels. In particular, the aerial parts of the species displayed a high cytotoxic effect (vitality%: 6.82 +/- 0.01 at 200 mg/mL) in breast cancer (MCF-7) cell line. Due to its remarkable antioxidant capacity, high content of rosmarinic, oleanolic, and ursolic acids and especially BChE, elastase, and collagenase inhibitory activity, T. pubescens has the potential for using in food supplements, food preservatives, cosmetics, and pharmaceutical industries.(c) 2022 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.sajb.2022.06.037
dc.identifier.endpage434en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85132874120
dc.identifier.scopusqualityQ1
dc.identifier.startpage425en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2022.06.037
dc.identifier.urihttps://hdl.handle.net/11468/16009
dc.identifier.volume149en_US
dc.identifier.wosWOS:000827840100004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSouth African Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectThymusen_US
dc.subjectGc-Msen_US
dc.subjectLc-Msen_US
dc.subjectMsen_US
dc.subjectOleanolicaciden_US
dc.subjectCytotoxicityen_US
dc.subjectEnzymeinhibitionen_US
dc.subjectEssentialoilen_US
dc.titleComprehensive study of chemical composition and biological activity of Thymus pubescens Boiss. et Kotschy ex Celaken_US
dc.titleComprehensive study of chemical composition and biological activity of Thymus pubescens Boiss. et Kotschy ex Celak
dc.typeArticleen_US

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