In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)

dc.contributor.authorKoc, Ibrahim
dc.contributor.authorOnay, Ahmet
dc.contributor.authorCiftci, Yelda Ozden
dc.date.accessioned2024-04-24T17:21:02Z
dc.date.available2024-04-24T17:21:02Z
dc.date.issued2014
dc.departmentDicle Üniversitesien_US
dc.description.abstractPistacia lentiscus L. (lentisk or mastic tree) is an economically important member of the genus Pistacia due to its valuable mastic resin. Shoot tips and nodal segments were used as explant sources from in vitro-germinated seeds of lentisk. Shoot tips were found to be suitable for multiple shoot formation. Thereafter, the influences of different growth regulators [N6-benzyladenine (BA), gibberellic acid (GA(3)), naphthalene acetic acid (NAA), or jasmonic acid (JA)] together with various elicitors [silver nitrate (AgNO3) or phloroglucinol (PG)] were assessed to develop efficient micropropagation protocol. Our results showed that the combination of all tested concentrations of GA(3) with 1.0 mg/L BA resulted in enhancement of multiple shoot formation. The maximum number of shoots per explant (3.95) was recorded on MS medium containing 1.0 mg/L BA and 0.3 mg/L GA(3). In contrast, JA had a negative influence, while AgNO3 had no significant effect on multiple shoot formation. In terms of synthetic seed production, it was possible to encapsulate shoot tips at 4 degrees C in darkness for up to 6 months with a frequency of 87.5% plant regrowth. In vitro-propagated microshoots, including plantlets derived from synthetic seeds, were transferred to MS medium containing different concentrations (1.0, 2.0, or 4.0 mg/L) of indole butyric acid for rooting and successfully acclimatized to ex vitro conditions. The presented data suggest an efficient in vitro regeneration system and conservation via synthetic seed production for lentisk.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [KBAG-110T941]en_US
dc.description.sponsorshipThis study was funded by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK, No. KBAG-110T941).en_US
dc.identifier.doi10.3906/biy-1401-69
dc.identifier.endpage663en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue5en_US
dc.identifier.startpage653en_US
dc.identifier.trdizinid163110
dc.identifier.urihttps://doi.org/10.3906/biy-1401-69
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/163110
dc.identifier.urihttps://hdl.handle.net/11468/19345
dc.identifier.volume38en_US
dc.identifier.wosWOS:000341523900014
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPistacia Lentiscus L.en_US
dc.subjectLentisken_US
dc.subjectMicropropagationen_US
dc.subjectPlant Growth Regulatorsen_US
dc.subjectSynthetic Seeden_US
dc.titleIn vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)en_US
dc.titleIn vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)
dc.typeArticleen_US

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