Development of Synthetic Seed Technology for the Conservation of Pistachio Germplasm
[ X ]
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Int Soc Horticultural Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Synthetic seed (synseed) technology using artificially encapsulated somatic embryos, shoot buds, cell aggregates, organogenic or embryogenic calli has become an important tool for micropropagation. Synseeds have multiple advantages for propagation including ease of handling, higher scale-up potential, low cost production, disease eradication, and potential medium- and long-term conservation. Because of those advantages, there are several papers published about slow growth storage and/or cryopreservation of different plant germplasms using encapsulated micropropagules. Although pistachio has important economical value, especially for Mediterranean countries where its largest cultivation is found, today it is under the threat of genetic erosion. Hence in this study, we aimed to develop efficient medium-term conservation procedure via encapsulation of shoot tips of Pistacia vera L. Uzun and Kirmizi as shoot tips have higher mitotic activity in the meristem. For the encapsulation procedure, shoot tips were mixed with 2.0 and 3.0% (w/v) sodium alginate (gelling agent) in liquid Murashige & Skoog (MS) medium including 0.09 or 0.4 M sucrose and transferred drop by drop to liquid MS medium supplemented with 100 mM calcium chloride (complexing agent) and held for 25 min for bead formation. Then, synseeds containing the shoot tips were transferred to MS medium supplemented with 1.0 mg L-1 benzyladenine (BA) and 0.5 mg L-1 gibberellic acid (GA(3)) for plant retrieval. As synseeds prepared with using 3.0% sodium alginate in MS medium including 0.4 M sucrose was more strict and gave higher regeneration percentages (100% for Uzun and Kirmizi), these concentrations of gelling agent were used for slow growth storage of pistachio shoot tips for 6, 9 and 12 months in dark at 4 degrees C. Studies are now underway to develop long-term conservation strategies of pistachio germplasm via encapsulation-vitrification and encapsulation-dehydration techniques.
Açıklama
5th International Symposium on Pistachios and Almonds -- OCT 06-10, 2009 -- Univ Harran, Sanliurfa, TURKEY
Anahtar Kelimeler
Encapsulation, P. Vera L., 'Kirmizi', Slow Growth Storage, 'Uzun'
Kaynak
V International Symposium on Pistachios and Almonds
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
912
