Evaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line

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dc.contributor.authorYasin, Diren Sarisaltik
dc.contributor.authorYilmaz, Sukran
dc.contributor.authorTeksin, Zeynep Safak
dc.date.accessioned2024-04-24T17:20:22Z
dc.date.available2024-04-24T17:20:22Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description.abstractBiorelevant media are used to simulate the physiological conditions in terms of components, pH, osmolality and buffer capacity of the human stomach and intestine in both fasted and fed states In this study, we aim to apply the biorelevant media to Caco-2 cell lines to investigate the cytotoxicity effects via the cell viability ratio and to compare the solubilizing effects of various dissolution media on a poorly soluble model drug. Flurbiprofen (Biopharmaceutics Classification System, BCS Class 2) was selected as a model drug. In dissolution studies the pH effects were predominant at higher pH values, while bile salt effects were dominant at lower pH values. The preparation method, bile salts and the phospholipids did not show any additional effect on Caco-2 cell viability. In the cytotoxicity test, fed state media caused an additional 10-15% decrease in cell viability, compared to the fasted state. Similar results were obtained when using the blank of these media which did not include the bile salt or phospholipid. From this, it is evident that this decrease resulted from the pH values, not the components. In conclusion, the cytotoxicity assessment showed that all the biorelevant media were compatible with 70-90% of cell viability for at least 24 h, and this ratio might be increased by modifying the pH.en_US
dc.description.sponsorshipGazi University Scientific Research Project [02/2008-20]en_US
dc.description.sponsorshipThis study was supported by Gazi University Scientific Research Project, Project No: 02/2008-20, 2008.en_US
dc.identifier.doi10.26650/IstanbulJPharm.2018.180013
dc.identifier.endpage88en_US
dc.identifier.issn2587-2087
dc.identifier.issue3en_US
dc.identifier.startpage82en_US
dc.identifier.trdizinid310715
dc.identifier.urihttps://doi.org/10.26650/IstanbulJPharm.2018.180013
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/310715
dc.identifier.urihttps://hdl.handle.net/11468/18993
dc.identifier.volume48en_US
dc.identifier.wosWOS:000456247200006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherIstanbul Univ, Fac Pharmacyen_US
dc.relation.ispartofIstanbul Journal of Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiorelevant Mediaen_US
dc.subjectFlurbiprofenen_US
dc.subjectCytotoxicityen_US
dc.titleEvaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell lineen_US
dc.titleEvaluation of biorelevant media to investigate the dissolution properties on flurbiprofen and to assess cytotoxicity effects on Caco-2 cell line
dc.typeArticleen_US

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