CTAB modified dellite: A novel support for enzyme immobilization in bio-based electrochemical detection and its in vitro antimicrobial activity

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dc.contributor.authorMaiga, Mohomodou
dc.contributor.authorYalcinkaya, Esra Evrim
dc.contributor.authorSonmez, Burak
dc.contributor.authorPuglia, Debora
dc.contributor.authorYavuz, Murat
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorKenny, Jose M.
dc.date.accessioned2024-04-24T16:18:22Z
dc.date.available2024-04-24T16:18:22Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractA novel support material for enzyme immobilization based on cetyltrimethylammonium bromide modified Dellite (CTAB-Del) was successfully synthesized and used to manufacture pyranose oxidase (PyOx) biosensors (CTAB-Del/PyOx). The intercalation of CTAB into Dellite was confirmed by FTIR, XRD and TGA techniques. PyOx was immobilized onto the glassy carbon electrode, via glutaraldehyde crosslinking, by using CTAB-Del as a support. In order to test the analytical performance of CTAB-Del/PyOx biosensors, chronoamperometric measurements were carried out using three electrodes configurations, at a constant potential of 0.7 V in working buffer, under stirring, with successive addition of glucose. The linear response for CTAB-Del/PyOx biosensor ranged from 0.01 to 0.50 mM with an equation of y=4.42x+0.004 (R-2=0.998), and the limit of detection for glucose was calculated to be 0.081 pM (S/N = 3). In order to confirm its practical use, the CTAB-Del/PyOx biosensor was also applied for glucose measurement in various beverages. In addition, the antimicrobial activities of Del and CTAB-Del were screened in vitro by means of the disc diffusion susceptibility test, selecting a yeast, three different Gram-positive, and five different Gram-negative bacteria. The obtained results showed a moderate antibacterial activity of CTAB-Del against Gram-positive bacteria. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipscientific research project (Ege University Project) [15-FEN-022]; Ege University AliyeUster Foundation; Turkish Academy of Sciences-Outstanding Young Scientists Award Program (TUBA-GEBIP)en_US
dc.description.sponsorshipThe authors would like to thank scientific research project (Ege University Project No: 15-FEN-022) and Ege University AliyeUster Foundation. Dr. D.O. Demirkol thanks The Turkish Academy of Sciences-Outstanding Young Scientists Award Program-2015 (TUBA-GEBIP).en_US
dc.identifier.doi10.1016/j.snb.2016.05.042
dc.identifier.endpage55en_US
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-84971260980
dc.identifier.scopusqualityQ1
dc.identifier.startpage46en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2016.05.042
dc.identifier.urihttps://hdl.handle.net/11468/16050
dc.identifier.volume235en_US
dc.identifier.wosWOS:000380823400007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDelliteen_US
dc.subjectCetyltrimethylammonium Bromide (Ctab)en_US
dc.subjectPyranose Oxidaseen_US
dc.subjectFunctionalized Surfaceen_US
dc.subjectBiosensoren_US
dc.subjectAntimicrobial Activityen_US
dc.titleCTAB modified dellite: A novel support for enzyme immobilization in bio-based electrochemical detection and its in vitro antimicrobial activityen_US
dc.titleCTAB modified dellite: A novel support for enzyme immobilization in bio-based electrochemical detection and its in vitro antimicrobial activity
dc.typeArticleen_US

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