Effect of genotype on somatic embryogenesis in pistachio (Pistacia vera L.)

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dc.contributor.authorOnay, Ahmet
dc.contributor.authorTilkat, Engin
dc.contributor.authorYildirim, Hakan
dc.date.accessioned2024-04-24T17:33:34Z
dc.date.available2024-04-24T17:33:34Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description5th International Conference on Propagation of Ornamental Plants -- SEP 05-08, 2007 -- Sofia, BULGARIAen_US
dc.description.abstractGenotypes representing eight botanical cultivars of pistachio (Pistacia vera L.) were assessed for somatic embryogenesis and subsequent plant conversion from immature kernels. Embryogenic masses (EMSs) were produced from kernels of immature fruit of pistachio cultured in liquid MS medium with Gamborg vitamins, supplemented with 100 mgl(-1) casein hydrolysate, 100 mgl(-1)L-Ascorbic acid, 3% sucrose, and BAP. EMS differentiated directly from the kernels after culture for 2 weeks in liquid medium with 0.5 mg P BAP. Genotype x collection date had large effects on initiation of somatic embryogenesis. Embryogenic tissues containing embryoid or individual somatic embryos were isolated from the original tissue and used to initiate repetitive liquid or agarified cultures. To determine the effects of genotypes during maturation on the subsequent germination and plantlet generation, clusters of EMSs from the different genotypes were transferred from liquid medium onto the surface of 5.7% agar solidified MS medium with Gamborg vitamins, containing 4% sucrose, 1.0 mg l(-1) BAP and 0.5 mg l(-1) IBA. After 4 weeks, individual or clusters of mature somatic embryos were transferred from maturation medium onto the germination medium (GM). The number of matured and germinated somatic embryos was influenced by the genotype in the maturation medium. Acclimatized plantlets resumed growth after transfer to a soil and peat mixture, and many developed to maturity.en_US
dc.identifier.endpage209en_US
dc.identifier.issn1311-9109
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-37149008284
dc.identifier.scopusqualityQ4
dc.identifier.startpage204en_US
dc.identifier.urihttps://hdl.handle.net/11468/20751
dc.identifier.volume7en_US
dc.identifier.wosWOS:000251493100008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSejani Publen_US
dc.relation.ispartofPropagation of Ornamental Plants
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenotype Effecten_US
dc.subjectPistachioen_US
dc.subjectPlant Regenerationen_US
dc.subjectSomatic Embryogenesisen_US
dc.titleEffect of genotype on somatic embryogenesis in pistachio (Pistacia vera L.)en_US
dc.titleEffect of genotype on somatic embryogenesis in pistachio (Pistacia vera L.)
dc.typeConference Objecten_US

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