The Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N mice strain on Escherichia coli LF82 Invasion

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dc.contributor.authorAygun, Husamettin
dc.contributor.authorKaramese, Murat
dc.contributor.authorUyar, Fikret
dc.date.accessioned2024-04-24T17:17:56Z
dc.date.available2024-04-24T17:17:56Z
dc.date.issued2019
dc.departmentDicle Üniversitesien_US
dc.description.abstractObjective: The aim of this study was to investigate the effect of healthy mucosa on adhesive and invasive properties of AIEC reference strain Escherichia cell (E. coli) LF82. For this purpose, we had designed special medias that contained cell culture medium and mucosal content obtained from different regions (colon and ileum) of the digestive tract. Methods: We tested the infecting ability of AIEC reference strain E. coli LF82 on 1-407 cells in the presence of mucosal media (Muc-M) under in ritro conditions. Muc-M composed of certain rates of cell culture medium or M63 minimal medium and mucosal contents obtained from different part of intestine were designed for cell-infection experiments and biofilm-formation assays. Results: The result showed that the mucosal media decreased the infection percentage of E. coli LF82 strain when compared with control group. It was seen that the mucosal media originating from ileum almost completely inhibited the invasion of LF82 strain. On the other hand, it was observed that the mucosal media prepared from colon) reduced the bacterial invasion only in half the rate when compared with control. Conclusion: The findings showed that these medias obtained from different regions of the intestinal tract affected LF82 invasion at different rates. Therefore, this study provided crucial information that could contribute to the future studies on the localization of bacteriaen_US
dc.identifier.doi10.14235/bas.galenos.2018.2627
dc.identifier.endpage203en_US
dc.identifier.issn2148-2373
dc.identifier.issue3en_US
dc.identifier.startpage198en_US
dc.identifier.trdizinid324515
dc.identifier.urihttps://doi.org/10.14235/bas.galenos.2018.2627
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/324515
dc.identifier.urihttps://hdl.handle.net/11468/18451
dc.identifier.volume7en_US
dc.identifier.wosWOS:000499482100005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherBezmialem Vakif Univen_US
dc.relation.ispartofBezmialem Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEscherichia Coli Lf82en_US
dc.subjectCrohn's Diseaseen_US
dc.subjectBiofilmen_US
dc.subjectPathobionten_US
dc.subjectAdhesionen_US
dc.subjectInvasionen_US
dc.titleThe Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N mice strain on Escherichia coli LF82 Invasionen_US
dc.titleThe Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N mice strain on Escherichia coli LF82 Invasion
dc.typeArticleen_US

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