INDIRECT SHOOT REGENERATION FROM IN VITRO-DERIVED ROOT AND LEAF EXPLANTS OF HYPERICUM SPECTABILE

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dc.contributor.authorIsikalan, Cigdem
dc.contributor.authorAkbas, Filiz
dc.contributor.authorNamli, Sureyya
dc.contributor.authorKarakus, Pinar
dc.date.accessioned2024-04-24T17:44:54Z
dc.date.available2024-04-24T17:44:54Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractAn applicable regeneration protocol through in vitro indirect organogenesis of Hypericum spectabile was developed. Leaf and root explants taken from in vitro grown seedlings were used as explants for callus induction. The explants were inoculated onto Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzilaminopurin (BAP; 0.5, 1.0, 1.5 mg l(-1)) and 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5, 1.0, 2.0 mg l(-1)). Callus initiation was observed in all media evaluated from leaf and root explants after two weeks (about 10-15 days) of culture. The best callus induction was observed in leaf explants in the treatment containing 1.0 mg l(-1) BAP + 2.0 mg l(-1) 2.4-D was compact-granular and green in color, while in the other treatments the calli were yellowish-light green and friable (loose). For shoot formation, calli were transferred into different BAP concentrations (0.25, 0.5, 1.0, 1.5 mg l(-1)) separately. Numerous shoots were obtained all media tested. Although the highest number of shoots produced on medium supplemented with 1.5 mg l(-1) BAP (25.15 shoots per explant), the best result was observed in the treatment containing 0.5 mg l(-1) BAP in terms of shoot development and morphogenic properties. The number of shoots produced was 22.75 per explant in these medium.en_US
dc.identifier.endpage2698en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue10Aen_US
dc.identifier.startpage2693en_US
dc.identifier.urihttps://hdl.handle.net/11468/22341
dc.identifier.volume20en_US
dc.identifier.wosWOS:000296690500006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCallus Inductionen_US
dc.subjectHypericum Spectabileen_US
dc.subjectIndirect Regenerationen_US
dc.subjectLeaf Explanten_US
dc.subjectPgrsen_US
dc.titleINDIRECT SHOOT REGENERATION FROM IN VITRO-DERIVED ROOT AND LEAF EXPLANTS OF HYPERICUM SPECTABILEen_US
dc.titleINDIRECT SHOOT REGENERATION FROM IN VITRO-DERIVED ROOT AND LEAF EXPLANTS OF HYPERICUM SPECTABILE
dc.typeArticleen_US

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