INDIRECT SHOOT REGENERATION FROM IN VITRO-DERIVED ROOT AND LEAF EXPLANTS OF HYPERICUM SPECTABILE
[ X ]
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An applicable regeneration protocol through in vitro indirect organogenesis of Hypericum spectabile was developed. Leaf and root explants taken from in vitro grown seedlings were used as explants for callus induction. The explants were inoculated onto Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzilaminopurin (BAP; 0.5, 1.0, 1.5 mg l(-1)) and 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5, 1.0, 2.0 mg l(-1)). Callus initiation was observed in all media evaluated from leaf and root explants after two weeks (about 10-15 days) of culture. The best callus induction was observed in leaf explants in the treatment containing 1.0 mg l(-1) BAP + 2.0 mg l(-1) 2.4-D was compact-granular and green in color, while in the other treatments the calli were yellowish-light green and friable (loose). For shoot formation, calli were transferred into different BAP concentrations (0.25, 0.5, 1.0, 1.5 mg l(-1)) separately. Numerous shoots were obtained all media tested. Although the highest number of shoots produced on medium supplemented with 1.5 mg l(-1) BAP (25.15 shoots per explant), the best result was observed in the treatment containing 0.5 mg l(-1) BAP in terms of shoot development and morphogenic properties. The number of shoots produced was 22.75 per explant in these medium.
Açıklama
Anahtar Kelimeler
Callus Induction, Hypericum Spectabile, Indirect Regeneration, Leaf Explant, Pgrs
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
20
Sayı
10A
