Kolesistektomi yapılan hastaların safra örneklerinin mikrobiyolojik değerlendirilmesi
Yükleniyor...
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Antibiyotik ve Kemoterapi Derneği
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Kolesistektomi, genel cerrahi kliniklerinde uygulanan en yaygın gastrointestinal operasyonlardan biridir. Bu çalışmada
akut veya kronik kolesistit nedeniyle kolesistektomi yapılan hastalardan alınan safra örneklerinde üreyen mikroorganizmaların
saptanması ve antibiyotik duyarlılıklarının belirlenmesi amaçlanmıştır. Üreyen aerob bakterilerin identifikasyonu ve antibiyotik duyarlılıkları Phoenix (Becton Dickinson Diagnostic Instrument Systems, Spark, Md, USA) otomatize sisteminde; anaerob bakterilerin identifikasyonu ise mini API (bioMerioux-Fransa) sisteminde gerçekleştirilmiştir.
İncelenen 108 safra örneğinin 36’sında (% 33) üreme saptanmıştır. Bu 36 örneğin 26’sında (% 72) Gram negatif
çomaklar (12 Escherichia coli, 8 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa, 2 Enterobacter cloacae); 8’inde (% 22)
Gram pozitif koklar (4 koagülaz negatif stafilokok, 2 Enterococcus faecalis, 2 Staphylococcus aureus) ve 2’sinde (% 6) anaerob
bakteri (Bacteroides fragilis) üremiştir. Hiçbir örnekte mantar ürememiş ve miks infeksiyon görülmemiştir. İzole edilen Gram
negatif bakterilere karşı amikasin (% 100), Gram pozitif bakterilere karşı ise vankomisin (% 100) en etkili antibiyotikler olarak
bulunmuştur.
Kolesistektomi sırasında alınan örneklerin mikrobiyolojik incelenmesi, post-operatif gelişen infeksiyonların tanı ve
tedavisini kolaylaştırmaktadır. Safra kültürünün değerlendirilmesi ve izole edilen etkene uygun antibiyotiğin başlanması
tedavi süresini kısaltacaktır.
Cholecystectomy is one of the most common gastrointestinal operations performed in general surgical units. The aim of this study was to determine the microorganisms and their antibiotic susceptibilities that were isolated from bile samples of patients who had cholecystectomy because of acute or chronic cholecystitis. The identification and antibiotic susceptibility of the isolated aerobic bacteria were performed by Phoenix (Becton Dickinson Diagnostic Instrument Systems, Spark, Md, USA) automated system and the identification of anaerobic bacteria was performed by mini API (bioMerioux-France) system. Of 108 bile samples examined, 36 (33 %) were culture positive. 26 (72 %) of which were Gram negative bacteria (12 Escherichia coli, 8 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa, 2 Enterobacter cloacae); 8 (22 %) were Gram positive cocci (4 coagulase negative staphylococci, 2 Enterococcus faecalis, 2 Staphylococcus aureus) and 2 (6 %) were anaerobic bacteria (Bacteroides fragilis). No fungi was isolated and there was no mixed infection. The most effective antibiotics against Gram negative and Gram positive bacteria were amikacin (100 %) and vankomycin (100 %), respectively. The microbiologic examination of the samples obtained during cholecystectomy, facilitates the diagnosis and treatment of post-operative infections. The evaluation of the bile culture and starting the appropriate antibiotic according to the isolated agent will shorten the treatment duration.
Cholecystectomy is one of the most common gastrointestinal operations performed in general surgical units. The aim of this study was to determine the microorganisms and their antibiotic susceptibilities that were isolated from bile samples of patients who had cholecystectomy because of acute or chronic cholecystitis. The identification and antibiotic susceptibility of the isolated aerobic bacteria were performed by Phoenix (Becton Dickinson Diagnostic Instrument Systems, Spark, Md, USA) automated system and the identification of anaerobic bacteria was performed by mini API (bioMerioux-France) system. Of 108 bile samples examined, 36 (33 %) were culture positive. 26 (72 %) of which were Gram negative bacteria (12 Escherichia coli, 8 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa, 2 Enterobacter cloacae); 8 (22 %) were Gram positive cocci (4 coagulase negative staphylococci, 2 Enterococcus faecalis, 2 Staphylococcus aureus) and 2 (6 %) were anaerobic bacteria (Bacteroides fragilis). No fungi was isolated and there was no mixed infection. The most effective antibiotics against Gram negative and Gram positive bacteria were amikacin (100 %) and vankomycin (100 %), respectively. The microbiologic examination of the samples obtained during cholecystectomy, facilitates the diagnosis and treatment of post-operative infections. The evaluation of the bile culture and starting the appropriate antibiotic according to the isolated agent will shorten the treatment duration.
Açıklama
Anahtar Kelimeler
Kolesistit, Safra kültürü, Bile culture, Cholecystitis
Kaynak
ANKEM Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
25
Sayı
4
Künye
Nergiz, Ş., Akpolat, N. ve Gül. K. (2011). Kolesistektomi yapılan hastaların safra örneklerinin mikrobiyolojik değerlendirilmesi. ANKEM Dergisi, 25(4), 227-231.
