Effects of some drugs on rat erythrocyte 6-phosphogluconate dehydrogenase

Basit Öğe Kaydı
dc.contributor.authorÇiftçi, M
dc.contributor.authorBeydemir, S
dc.contributor.authorYilmaz, H
dc.contributor.authorBakan, E
dc.date.accessioned2024-04-24T17:47:32Z
dc.date.available2024-04-24T17:47:32Z
dc.date.issued2002
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe in vitro and in vivo effects of some drugs on rat erythrocytes 6-phosphogluconate dehydrogenase were investigated in this study. Rat erythrocyte 6-phosphogluconate dehydrogenase was partially purified with ammonium sulfate precipitation. The enzyme activity was determined by Beutler's method. Some drugs such as ampicillin, amikacin sulfate, and netilmicin sulfate inhibited the enzyme activity in in vitro conditions, while metamizole activated it. The I-50 values of the inhibiting drugs were 66.2, 5.836, and 0.963 mM, respectively. For the drugs having low I-50 values (drug concentrations which produce 50% inhibition) (amikacin sulfate and netilmicin sulfate), in vivo studies were performed in rats (Sprague-Dawley). Amikacin sulfate at 64 mg/kg inhibited the enzyme activity significantly (p < 0.05) 2 h after dosing. Netilmicin sulfate at 6.4 mg/kg also inhibited the enzyme significantly (p < 0.05) 4 h after dosing. Amikacin sulfate and netilmicin sulfate inhibited rat erythrocyte 6-phospogluconate dehydrogenase both in vivo and in vitro. The enzyme was inhibited in vitro by ampicillin and activated in vitro by metamizole.en_US
dc.identifier.endpage280en_US
dc.identifier.issn1230-6002
dc.identifier.issue3en_US
dc.identifier.pmid12398160
dc.identifier.startpage275en_US
dc.identifier.urihttps://hdl.handle.net/11468/22576
dc.identifier.volume54en_US
dc.identifier.wosWOS:000177272200010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherPolish Acad Sciences Inst Pharmacologyen_US
dc.relation.ispartofPolish Journal of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject6-Phospogluconate Dehydrogenaseen_US
dc.subjectErythrocytesen_US
dc.subjectRaten_US
dc.subjectDrugsen_US
dc.titleEffects of some drugs on rat erythrocyte 6-phosphogluconate dehydrogenaseen_US
dc.titleEffects of some drugs on rat erythrocyte 6-phosphogluconate dehydrogenase
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu