Purification and characterization of alkaline proteinase from alkalophilic Bacillus sp.

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dc.contributor.authorMuderriszade, A
dc.contributor.authorEnsari, NY
dc.contributor.authorAguloglu, S
dc.contributor.authorOtludil, B
dc.date.accessioned2024-04-24T16:19:09Z
dc.date.available2024-04-24T16:19:09Z
dc.date.issued2001
dc.departmentDicle Üniversitesien_US
dc.description.abstractAlkaline proteinase was purified from Bacillus sp. isolated from soil. The pH optimum was 11.5 at 37 degreesC. Calcium divalent cation was effective in stabilizing the enzyme, especially at higher temperatures. The proteolytic activity was inhibited by the specific serine proteinase inhibitor PMSF (phenylmethylsulfonyl fluoride), and ions of Mg, Mn, Pb, Li, Zn, Ag, and Hg. The enzyme was stable in the presence of detergents, such as Triton-X100, Tween-80. SDS (sodium dodecyl sulfate), and EDTA (ethylenediaminetetraacetic acid), at pH 11.5 and 37 degreesC for 30 min. The optimum pH was 11.5 at 37 degreesC, and the optimum temperature was 62 degreesC at pH 11.5.en_US
dc.identifier.doi10.1023/A:1012346916124
dc.identifier.endpage577en_US
dc.identifier.issn0003-6838
dc.identifier.issn1573-8183
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-27144510361
dc.identifier.scopusqualityQ4
dc.identifier.startpage574en_US
dc.identifier.urihttps://doi.org/10.1023/A:1012346916124
dc.identifier.urihttps://hdl.handle.net/11468/16416
dc.identifier.volume37en_US
dc.identifier.wosWOS:000172177000005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherMaik Nauka/Interperiodica/Springeren_US
dc.relation.ispartofApplied Biochemistry and Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titlePurification and characterization of alkaline proteinase from alkalophilic Bacillus sp.en_US
dc.titlePurification and characterization of alkaline proteinase from alkalophilic Bacillus sp.
dc.typeArticleen_US

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