Molecular characterization of rhizospheric soil streptomycetes isolated from indigenous Turkish plants and their antimicrobial activity

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dc.contributor.authorYilmaz, Ebru Ince
dc.contributor.authorYavuz, Murat
dc.contributor.authorKizil, Murat
dc.date.accessioned2024-04-24T16:02:26Z
dc.date.available2024-04-24T16:02:26Z
dc.date.issued2008
dc.departmentDicle Üniversitesien_US
dc.description.abstractAs part of a research program whose aim is to determine the diversity of streptomycetes in order to discover new bioactive secondary metabolites, rhizosphere soils of three indigenous plants were analyzed. A total of 55 actinomycetes were isolated using three different medium from the samples. The rhizospheric soil of the plant Aethionema dumanii gave the highest number of actinomycetes, i.e., 42% versus 27% and 31% for the soils from Salvia aytachii and Achillea ketenoglui, respectively. The AIA is the most favorable medium for the isolation of the actinomycetes from different rhizospheric soils. 16S rDNA sequence analysis revealed that while some isolates belong to different cluster groups such as Streptomyces lydicus, S. rochei, S. microflavus, S. griseoflavus, S. albidoflavus and S. violaceusniger, the majority of the sequences did not considerable clustered with the member of different Streptomyces groups. The in vitro antimicrobial activities of the crude organic and aqueous extracts of isolates were screened using a disc diffusion assay against a panel of bacteria and C. albicans. A total of 22 isolates showed antimicrobial activity. The antibacterial action of the extracts is more pronounced on Gram-positive than on Gram-negative bacteria in most cases. About 18% of the actinomycetes showed also antifungal activity. Study of the influence of two different culture media on production of bioactive molecules showed that the higher antimicrobial activity was obtained in M2 when compared to TSB. The results from this study provide evidence that the streptomycetes in the rhizosphere soils could be promising sources for antimicrobial bioactive agents.en_US
dc.identifier.doi10.1007/s11274-007-9628-8
dc.identifier.endpage1470en_US
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-45849091551en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1461en_US
dc.identifier.urihttps://doi.org/10.1007/s11274-007-9628-8
dc.identifier.urihttps://hdl.handle.net/11468/14791
dc.identifier.volume24en_US
dc.identifier.wosWOS:000256915600027
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofWorld Journal of Microbiology & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectIdentificationen_US
dc.subjectRhizosphere Soilen_US
dc.subjectSelective Isolationen_US
dc.subjectStreptomycetesen_US
dc.subject16s Rrna Geneen_US
dc.titleMolecular characterization of rhizospheric soil streptomycetes isolated from indigenous Turkish plants and their antimicrobial activityen_US
dc.typeArticleen_US

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