Expression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasma

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dc.contributor.authorUcar, Eyyup Hakan
dc.contributor.authorHitit, Mustafa
dc.contributor.authorKose, Mehmet
dc.contributor.authorAtli, Mehmet Osman
dc.date.accessioned2024-04-24T17:18:08Z
dc.date.available2024-04-24T17:18:08Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn the current study, we aimed to assess the expression levels of two circulating microRNAs (miRNA) (oar-miR-485-5p and oar-miR-493-5p) in the ovine plasma during the peri-implantation. After mating, we collected the plasma samples from a total of 8 ewes on day 22 of pregnancy (P22; n = 4) and day 22 of the estrous cycle (C22; n=4). We used mature miRNA sequences for oar-miR-485-5p and oar-miR-493-5p out of one hundred fifty, which were retrieved from our microarray results of previous study. We showed that the miRNA expression of oar-miR-485-5p and oar-miR-493-5p were upregulated in P22 (P<0.05) when compared to C22. Those two miRNAs targeted 311 target genes in the peri-implantation period of pregnancy. Furthermore, we revealed 151 GO/pathway terms in biological process (BP) and 25 GO/pathway terms in molecular function (MF), while we demonstrated 13 GO/pathway terms in cellular component (CC). We revealed three hub genes as interleukin 2 (IL2), interleukin 18 (IL18), and C -X-C Motif Chemokine Ligand 10 (CXCL10). In conclusion, both miR-485-5p and oar-miR-493-5p have the potential to be a biomarker to understand peri-implantation of the ovine pregnancy in the aspect of pregnancy -reflected changes in maternal plasma.en_US
dc.description.sponsorshipDepartment of Biomedical Sciences, College of Veterinary Medicine; Scientific and Technological Research Council of Turkey (TUBITAK) [214O643]en_US
dc.description.sponsorshipThe authors thank Dr. Aydin Guzeloglu (Department of Biomedical Sciences, College of Veterinary Medicine) for the support. The study was financially funded by Scientific and Technological Research Council of Turkey (TUBITAK) projects (214O643) to MK.en_US
dc.identifier.doi10.1590/1984-3143-AR2023-0115
dc.identifier.issn1806-9614
dc.identifier.issn1984-3143
dc.identifier.issue1en_US
dc.identifier.pmid38510567
dc.identifier.urihttps://doi.org/10.1590/1984-3143-AR2023-0115
dc.identifier.urihttps://hdl.handle.net/11468/18630
dc.identifier.volume21en_US
dc.identifier.wosWOS:001179867800001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBrazilian Coll Animal Reproductionen_US
dc.relation.ispartofAnimal Reproduction
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicrornaen_US
dc.subjectPlasmaen_US
dc.subjectOar-Mir-485-5pen_US
dc.subjectOar-Mir-493-5pen_US
dc.subjectOvineen_US
dc.titleExpression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasmaen_US
dc.titleExpression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasma
dc.typeArticleen_US

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