Micropropagation of Pistacia lentiscus L. from axenic seedling-derived explants

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dc.contributor.authorYildirim, Hakan
dc.date.accessioned2024-04-24T16:18:22Z
dc.date.available2024-04-24T16:18:22Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractPistacia lentiscus L. (Anacardiaceae). a deciduous forest tree, is important multipurpose Pistacia tree. Today, the major limitation facing the widespread expansion of commercial lentisk plantations is the shortage of superior plants primarily because of difficulties experienced in propagating this species by using the traditional vegetative propagation methods. Shoot cultures were established from aseptically germinated seedlings of lentisk. Factors including the different N-6-benzyladenine (BA) concentrations, the combination of cytokinin and other growth regulators, media and antioxidants were assessed and optimized for in vitro shoot proliferation. Full strength MS medium with Gamborg's vitamins containing 30 gl(-1) sucrose, 100 mg l(-1) PVP, 1 mg l(-1) BA and 7 g l(-1) agar resulted in multiple shoot (bud) initiation at the rate of 2.7 +/- 0.17 shoot (4.18 +/- 0.17 bud) per explant in 28 days of culture. Moreover, with the use of in vitro proliferated axenic cultures subcultured at least twice, the effects of auxins and mineral medium strength were also assessed for root induction. Efficient rooting (92%) was achieved in a medium containing 1 mg l(-1) indole-3-butyric acid (IBA). The method developed for plant acclimatization was satisfactory because a high percentage of plant survival (83.33%) in the growth room was obtained and the regenerated plantlets resumed growth after 4 months (96%). The method described will be useful for rapid multiplication of lentisk for commercial exploitation. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK - The Scientific and Technical Research Council of Turkey [TBAG-110T941]; Dicle University [DUBAP-10-ZF-26]en_US
dc.description.sponsorshipThe study was funded by grant #TBAG-110T941 from TUBITAK - The Scientific and Technical Research Council of Turkey and Dicle University The Scientific Research Projects # DUBAP-10-ZF-26 #. The authors are also grateful to Tansu AVCI for providing the seeds of Pistacia lentiscus L.en_US
dc.identifier.doi10.1016/j.scienta.2012.01.020
dc.identifier.endpage35en_US
dc.identifier.issn0304-4238
dc.identifier.issn1879-1018
dc.identifier.scopus2-s2.0-84856795812en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage29en_US
dc.identifier.urihttps://doi.org/10.1016/j.scienta.2012.01.020
dc.identifier.urihttps://hdl.handle.net/11468/16039
dc.identifier.volume137en_US
dc.identifier.wosWOS:000302521500004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofScientia Horticulturaeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLentisken_US
dc.subjectMastic Treeen_US
dc.subjectMedium Compositionen_US
dc.subjectOrganogenesisen_US
dc.titleMicropropagation of Pistacia lentiscus L. from axenic seedling-derived explantsen_US
dc.typeArticleen_US

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