Harnessing silk fibroin microparticles for metformin delivery: A novel approach to treating corneal neovascularization

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dc.contributor.authorPolat, Heybet Kerem
dc.contributor.authorAytekin, Eren
dc.contributor.authorKarakuyu, Nasıf Fatih
dc.contributor.authorÇaylı, Yağmur Akdağ
dc.contributor.authorÇalamak, Semih
dc.contributor.authorDemirci, Nazire
dc.contributor.authorMokhtare, Behzad
dc.date.accessioned2024-04-24T17:56:09Z
dc.date.available2024-04-24T17:56:09Z
dc.date.issued2024
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Patoloji Ana Bilim Dalıen_US
dc.description.abstractCorneal neovascularization (CV) poses significant challenges in ophthalmology, often leading to impaired vision and discomfort. Silk fibroin microparticles have emerged as promising candidates for drug delivery applications, owing to their biocompatibility, biodegradability, and controlled release characteristics. In the current research, metformin-loaded silk microparticles were developed, and their potential for treating corneal neovascularization was evaluated. Silk microparticles were prepared using silk fibroin and polyvinyl alcohol (PVA) at various weight ratios, facilitating phase separation to create a porous structure conducive to drug loading. Metformin (MT), a widely used antidiabetic agent with potential anti-angiogenic properties, was incorporated into the silk microparticles at different concentrations. The formulation termed TB (0.3 % MT loaded Microparticles) emerged as the most promising candidate was identified through comprehensive in vitro evaluations. In in vitro cytotoxicity studies, it was determined that all formulations provided cell viability above 80%, however, in permeability studies and encapsulation efficiency studies, TB was determined to be more effective than other formulations. TB demonstrated a particle size of approximately 8.9 ± 3.6 ?m and an encapsulation efficiency of 96.3 ± 2.1%. In vitro release studies revealed that TB exhibited a burst release of 62% within the initial two days, followed by sustained release over a period of 14 days, consistent with Peppas-Sahlin kinetics. Mathematical modeling further corroborated the diffusion mechanism underlying drug release from TB microparticles. In vivo studies on rats and histochemical analyzes on corneas showed that TB exhibited efficacy comparable to dexamethasone, a standard treatment for CV, despite being administered once daily. Histological analyses of corneal tissues revealed reduced neovascularization and inflammation in the TB-treated group, underscoring its therapeutic potential. Overall, our findings highlight the promise of metformin-loaded silk fibroin microparticles as a novel therapeutic approach for managing CV, offering sustained drug release and enhanced efficacy in treating this challenging ocular condition. © 2024en_US
dc.identifier.citationPolat, H. K., Aytekin, E., Karakuyu, N. F., Çaylı, Y. A., Çalamak, S., Demirci, N. ve diğerleri. (2024). Harnessing silk fibroin microparticles for metformin delivery: A novel approach to treating corneal neovascularization. Journal of Drug Delivery Science and Technology, 96, 1-11.
dc.identifier.doi10.1016/j.jddst.2024.105625
dc.identifier.issn1773-2247
dc.identifier.scopus2-s2.0-85189791325
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2024.105625
dc.identifier.urihttps://hdl.handle.net/11468/23323
dc.identifier.volume96en_US
dc.indekslendigikaynakScopus
dc.institutionauthorMokhtare, Behzad
dc.language.isoenen_US
dc.publisherEditions de Santeen_US
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCorneal neovascularen_US
dc.subjectMetforminen_US
dc.subjectMicroparticleen_US
dc.subjectPeppas-sahlinen_US
dc.subjectSilk fibroinen_US
dc.titleHarnessing silk fibroin microparticles for metformin delivery: A novel approach to treating corneal neovascularizationen_US
dc.titleHarnessing silk fibroin microparticles for metformin delivery: A novel approach to treating corneal neovascularization
dc.typeArticleen_US

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