In vitro antioxidant, anticholinesterase, tyrosinase activity studies, and LC-MS/MS simultaneous determination of 37 bioactive compounds in Indigofera heterantha

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dc.contributor.authorUllah, Zain
dc.contributor.authorAli, Saqib
dc.contributor.authorHussain, Atarad
dc.contributor.authorOzturk, Mehmet
dc.contributor.authorERTAS, Abdulselam
dc.contributor.authorAlamzeb, Muhammad
dc.contributor.authorRashid, Mamoon Ur
dc.date.accessioned2024-04-24T16:18:20Z
dc.date.available2024-04-24T16:18:20Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe flowering plant Indigofera heterantha generally known as Indigo of Himalayan, was extracted with nhexane, ethyl acetate, and methanol by using Soxhlet apparatus. The extracts were studied for their antioxidants and enzyme inhibition activities. Against DPPH free radical-scavenging activity methanol extract was found moderately active (IC50: 158.41 +/- 1.1.66 mu g/mL). The ethyl acetate and n-hexane extracts exhibited the best ABTS radical scavenging activity (IC50: 31.32 +/- 2.50 and 52.0 +/- 0.89 mu g/mL respectively). Against the metal chelating activity, n-hexane extract was active IC50: 52.36 +/- 0.95 mu g/mL. The n-hexane and ethyl acetate extracts demonstrated the best b-carotene antioxidant activity (IC50: 22.32 +/- 0.84 and 60.66 +/- 0.35 mu g/mL respectively). Against acetylcholinesterase (AChE), ethyl acetate and methanol extracts were moderate active (IC50: 133.58 +/- 0.870, and 137.97 +/- 0.97 mu g/mL respectively). The ethyl acetate extract showed Butyrylcholinesterase (BChE) inhibitory activity with IC50 value of 121.9 +/- 1.13 mu g/mL. Against tyrosinase activity, ethyl acetate and n-hexane extracts activity with an IC50 value of 79.25 +/- 0.15 and 131.48 +/- 0.88 mu g/mL respectively. The phenolic contents of the methanolic extract of I. heterantha were also determined by using LC-MS/MS. The major constituents were Quinic acid (57,333.04 mu g/g), malic acid (1135.16 mu g/g), gallic acid (201.13 mu g/g), isoquercitrin (113.63 mu g/g), Rutin (92.97 mu g/g), and Salicylic acid (40.45 mu g/g). Keeping in view the overall biological activities of I. heterantha, it might be used in food industries and pharmaceuticals as a potential functional food ingredient.(c) 2022 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK [TUBITAK-BIDEB-2215]en_US
dc.description.sponsorshipThe authors are grateful to the Department of Chemistry, Mohi-Ud-Din Islamic University, Nerian Sharif, Azad Jammu and Kashmir-Pakistan and Department of Chemistry, Mugla Sitki Kocman Univer-sity, Mugla, Turkey, for providing basic laboratory facilities. One of the author Z. Ullah, also like to thank TUBITAK for supporting the grant, namely, PhD. Fellowship Programme for Foreign Citizens (TUBITAK-BIDEB-2215) .en_US
dc.identifier.doi10.1016/j.sajb.2022.05.012
dc.identifier.endpage545en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85130596206
dc.identifier.scopusqualityQ1
dc.identifier.startpage537en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2022.05.012
dc.identifier.urihttps://hdl.handle.net/11468/16008
dc.identifier.volume148en_US
dc.identifier.wosWOS:000806370000017
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSouth African Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIndigofera Heteranthaen_US
dc.subjectAntioxidant Activityen_US
dc.subjectAnticholinesterase Activityen_US
dc.subjectTyrosinase Inhibition Assayen_US
dc.subjectLc-Msen_US
dc.subjectMsen_US
dc.titleIn vitro antioxidant, anticholinesterase, tyrosinase activity studies, and LC-MS/MS simultaneous determination of 37 bioactive compounds in Indigofera heteranthaen_US
dc.titleIn vitro antioxidant, anticholinesterase, tyrosinase activity studies, and LC-MS/MS simultaneous determination of 37 bioactive compounds in Indigofera heterantha
dc.typeArticleen_US

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