Evaluation of Silybum marianum seed extract and vitamin B6 derivatives on methylglyoxal and sugar-induced oxidative DNA damage

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dc.contributor.authorInceoren, N.
dc.contributor.authorToptanci, B. Ceken
dc.contributor.authorKizil, G.
dc.contributor.authorKizil, M.
dc.date.accessioned2024-04-24T17:18:04Z
dc.date.available2024-04-24T17:18:04Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstractReducing sugars are known to generate reactive oxygen species (ROS), mainly by means of the glycation reaction. The hydroxyl radical, a prominent entity of ROS, is known to alter cellular DNA and induces damage to DNA, and plays a role in diseases such as diabetes mellitus. In this study, the oxidative damage of DNA induced by the lysine/Fe3 thorn /MG reaction was investigated. Silybum marianum seeds extract (SlyE), standard silymarin (Sly), and vitamin B6 derivatives, pyridoxal-5-phosphate (PLP), pyridoxamine (PM), and pyridoxine (P) in reversing glycation-induced damage in DNA were evaluated. In addition, different sugars and sugar phosphates were incubated with plasmid pBR 322 DNA to control and compare their harmful effects. Our results revealed that SlyE protected lysine/Fe3 thorn /MG induced oxidative DNA damage more effectively than Sly. Vitamins, on the other hand, prevented this DNA damage in the order of PLP>P>PM. The DNA altering and damaging intensity of sugars and sugar phosphates tested increased considerably in the following order: Ribose-5-phosphate > fructose-6-phosphate > ribose > fructose > fructose-1,6 biphosphate > glucose-6 phosphate > glucose. The results show that the lysine/Fe3 thorn /MG glycation reaction can cause oxidative damage of DNA through a mechanism involving hydroxyl radicals. It also provides evidence that ribose-5-phosphate and fructose and its phosphate metabolites can alter DNA more rapidly in vitro than glucose and its phosphate metabolites.en_US
dc.description.sponsorshipDicle University Scientific Research Projects Coordinator (DUBAP) [Fen 15008]en_US
dc.description.sponsorshipThis work was supported by grants from the Dicle University Scientific Research Projects Coordinator (DUBAP-Project No: Fen 15008).en_US
dc.identifier.doi10.1556/066.2022.00138
dc.identifier.endpage51en_US
dc.identifier.issn0139-3006
dc.identifier.issn1588-2535
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85148532626
dc.identifier.scopusqualityQ3
dc.identifier.startpage40en_US
dc.identifier.urihttps://doi.org/10.1556/066.2022.00138
dc.identifier.urihttps://hdl.handle.net/11468/18587
dc.identifier.volume52en_US
dc.identifier.wosWOS:001040496600004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherAkademiai Kiado Zrten_US
dc.relation.ispartofActa Alimentaria
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSilybum Marianumen_US
dc.subjectMethylglyoxalen_US
dc.subjectHydroxyl Radicalen_US
dc.subjectDna Cleavageen_US
dc.subjectOxidative Dna Damageen_US
dc.titleEvaluation of Silybum marianum seed extract and vitamin B6 derivatives on methylglyoxal and sugar-induced oxidative DNA damageen_US
dc.titleEvaluation of Silybum marianum seed extract and vitamin B6 derivatives on methylglyoxal and sugar-induced oxidative DNA damage
dc.typeArticleen_US

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