A comparative investigation of the effects of Resveratrol and dental pulp delivered mesenchimal stem cells on rat tibia bone defect healing

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dc.contributor.authorAgin, Hatice Demircan
dc.contributor.authorGunes, Nedim
dc.contributor.authorGuler, Ridvan
dc.date.accessioned2025-02-22T14:08:36Z
dc.date.available2025-02-22T14:08:36Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractResveratrol (3,4,5-trihydroxystilbene), an antioxidant compound, has a natural phytoalexin structure and also has many properties such as anti-inflammatory, antineoplastic and antiplatelet. In addition, mesenchymal stem cells isolated from various tissues are considered as a potential cell source for bone regenerative therapies. The present study aims to examine the effects of Resveratrol and dental pulp- derived mesenchymal stem cells on new bone formation in rats, both isolated and combined, by immunohistochemical methods. Twenty eigth Spraque Dawley male rats were used in the study. The rats were divided into four groups with seven rats in each group; the control group (Group 1) (n=7), the Systemic Resveratrol group (Group 2) (n=7), the Stem cell group (Group 3) (n=7), the Stem cell + Systemic Resveratrol group (Group 4) (n=7). A defect was opened on the tibia bones of the rats in all groups with a trephane bur (diameter of 3 mm and a length of 4 mm). After the 4-week experiment, all rats were sacrificed following the experimental protocols specific to each group. The specimens of tibia were subjected to histomorphological examination in fixative solutions. Values of inflammation, connective tissue formation, osteoclastic activity, osteoblast values, new bone formation, BMP2 and BMP4 expression levels obtained for all groups were evaluated by statistical analysis. Compared to the control group, new bone formation and osteoblastic activity were found to be significantly higher in the Stem cell group and Stem cell + Systemic Resveratrol group. ( P =0.001) Additionally, new bone formation in the Systemic Resveratrol group was found to be significantly lower than in the Stem cell + Systemic Resveratrol group. ( P =0.006) No significant difference was observed between other groups. ( P >0.05) According to the results of the study, it was observed that Stem cell + Resveratrol treatment was more effective than isolated Resveratrol or isolated stem cell treatment applications, it induced the development of more bone trabeculae, decrease inflammation and increased the number of osteoblasts involved in bone formation. In the light of these data, it was concluded that the combined use of Resveratrol and Stem cells is more effective on the healing of bone defects than their isolated use.en_US
dc.description.sponsorshipDicle University Scientific Research Projects Coordination Office [DIdot;Scedil;.20.007]en_US
dc.description.sponsorshipThis study is supported by Dicle University Scientific Research Projects Coordination Office with project number D & Idot;& Scedil;.20.007.en_US
dc.identifier.doi10.52973/rcfcv-e34372
dc.identifier.issn0798-2259
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85196023329en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.52973/rcfcv-e34372
dc.identifier.urihttps://hdl.handle.net/11468/29508
dc.identifier.volume34en_US
dc.identifier.wosWOS:001278422800001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherUniv Zulia, Facultad Ciencias Veterinariasen_US
dc.relation.ispartofRevista Cientifica-Facultad De Ciencias Veterinariasen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectBone defecten_US
dc.subjectResveratrolen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectraten_US
dc.subjectexperimental studyen_US
dc.titleA comparative investigation of the effects of Resveratrol and dental pulp delivered mesenchimal stem cells on rat tibia bone defect healingen_US
dc.typeArticleen_US

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