Application of disposable biosensor for nivolumab–DNA interaction using pencil graphite electrode

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dc.authorid0000-0002-8114-4940en_US
dc.authorid0000-0002-0868-2769en_US
dc.authorid0000-0001-5792-419Xen_US
dc.contributor.authorAslan, Mehmet
dc.contributor.authorAydın, Fırat
dc.contributor.authorAslan, Ferhat
dc.contributor.authorLevent, Abdulkadir
dc.date.accessioned2024-03-11T12:52:50Z
dc.date.available2024-03-11T12:52:50Z
dc.date.issued2023en_US
dc.departmentDicle Üniversitesi, Fen Fakültesi, Kimya Bölümüen_US
dc.description.abstractAbstract: In this study, the interaction of the anticancer drug Nivolumab (NIVO) with dsDNA was investigated using differential pulse (DPV) technique by monitoring the changes in the oxidation signal of adenine and guanine bases on PG electrode. Surface characterization of the PG (pencil graphite) electrode was carried out using cyclic voltammetry, electrochemical impedance spectrometry techniques and scanning electron microscope, Energy Dispersive X-ray. DPV parameters, the effect of pH on the electrode surface, stirring speed, dsDNA immobilization time, immobilized dsDNA concentrations, NaCl concentration, NIVO concentration interacting with dsDNA were optimized with PG electrode. With the determined parameters, NIVO–dsDNA interaction in PBS (pH 3.0) supporting electrolyte solution was elucidated. In the NIVO–dsDNA interaction using UV technique, the K binding constant and free energy of binding value was found to be 8.93 × 104 and 6.754 kcal mol–1, respectively. It was determined that the data obtained by electrochemical method were in parallel with the data obtained by spectrometric method. The results obtained electrochemically with high sensitivity, low cost, fast, simple, low detection limit will be the first example of NIVO–dsDNA interaction using PG electrode.en_US
dc.identifier.citationAslan, M., Aydın, F., Aslan, F. ve Levent, A. (2023). Application of disposable biosensor for nivolumab–DNA interaction using pencil graphite electrode. Russian Journal of Electrochemistry, 59(11), 941-953.en_US
dc.identifier.doi10.1134/S1023193523110046
dc.identifier.endpage953en_US
dc.identifier.issn1023-1935
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85176949117
dc.identifier.scopusqualityQ4
dc.identifier.startpage941en_US
dc.identifier.urihttps://link.springer.com/article/10.1134/S1023193523110046
dc.identifier.urihttps://hdl.handle.net/11468/13568
dc.identifier.volume59en_US
dc.identifier.wosWOS:001105120200007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAslan, Mehmet
dc.institutionauthorAydın, Fırat
dc.language.isoenen_US
dc.publisherPleiades Publishingen_US
dc.relation.ispartofRussian Journal of Electrochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticanceren_US
dc.subjectDNA interactionen_US
dc.subjectNivolumaben_US
dc.subjectPGEen_US
dc.titleApplication of disposable biosensor for nivolumab–DNA interaction using pencil graphite electrodeen_US
dc.titleApplication of disposable biosensor for nivolumab–DNA interaction using pencil graphite electrode
dc.typeArticleen_US

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