Comparison of different fixatives effects in histochemical stainings of peripheral nerve tissue

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dc.contributor.authorDörtbudak, Muhammet Bahaeddin
dc.contributor.authorDemircioğlu, Muhammed
dc.contributor.authorŞeker, Uğur
dc.contributor.authorDemircioğlu, İsmail
dc.date.accessioned2025-02-22T14:10:55Z
dc.date.available2025-02-22T14:10:55Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractA pathological condition in the peripheral nerve tissue, which provides the connection between the organism and the external environment, negatively affects the standard of living. The nerve tissue histotechnology is of serious importance both for scientific studies and for clinical diagnosis. The fixation, which is one of the leading procedures for histological examination of tissues, aims to preserve tissue morphology. Another essential part of the histological examination is staining process. This study, it was aimed to determine the fixative that provides optimal histological appearance in peripheral nerve tissue. Therefore, various histochemical stainings of tissues fixed with some fixatives used in practice were compared. Sciatic nerves from each rat (n=7) used in the study were fixed with different fixatives and histochemical staining was performed. In histological examination, cellular (nucleus-cytoplasm) and intercellular morphological details, staining intensity and distribution were evaluated. At the end of the study, formaldehyde was found to be the most ideal fixing agent for all stains. Although Bouin and Carnoy fixatives differed according to the staining type, their fixation quality was similar in general. Glutaraldehyde did not give as good results as other fixatives in all stainings. This study is an important technical reference for clinical and experimental studies. © 2024 Cellular and Molecular Biology Association. All rights reserved.en_US
dc.identifier.doi10.14715/cmb/2024.70.12.12
dc.identifier.endpage94en_US
dc.identifier.issn0145-5680
dc.identifier.issue12en_US
dc.identifier.pmid39799492en_US
dc.identifier.scopus2-s2.0-85214817632en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage88en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2024.70.12.12
dc.identifier.urihttps://hdl.handle.net/11468/29891
dc.identifier.volume70en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCellular and Molecular Biology Associationen_US
dc.relation.ispartofCellular and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_Scopus_20250222
dc.subjectFixationen_US
dc.subjectHistochemical stainingen_US
dc.subjectHistotechnologyen_US
dc.subjectNerve tissueen_US
dc.subjectSciatic nerveen_US
dc.titleComparison of different fixatives effects in histochemical stainings of peripheral nerve tissueen_US
dc.typeArticleen_US

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