Mass shoot proliferation of pistacia khinjuk stocks using temporary immersion bioreactor system (TIS)

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dc.contributor.authorTilkat, Engin
dc.contributor.authorSüzerer, Veysel
dc.contributor.authorErsali, Yusuf
dc.contributor.authorHoşer, Ayşe
dc.contributor.authorKılınç, Fatih Mehmet
dc.contributor.authorTilkat, Emine Ayaz
dc.contributor.authorAkdemir, Hülya
dc.contributor.authorOnay, Ahmet
dc.date.accessioned2024-04-24T17:56:50Z
dc.date.available2024-04-24T17:56:50Z
dc.date.issued2014
dc.departmentDicle Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractA temporary immersion bioreactor system (TIS), compared to semi-solid systems has many advantages such as (1) the system can be controlled by an automation system; (2) a greater number of explants could be used at the same time and (3) there is no need to use an organic gelling agent. In this study, we aimed to develop a method suitable for mass shoot proliferation of P. khinjuk Stocks. Shoot tips and nodal segments of in vitro grown axenic regenerates derived from seedlings of Pistacia khinjuk Stocks were used as material. In both semi-solid and liquid medium experiments, an MS medium supplemented with 1 mg/L BA and 30 g/L sucrose was used. Various immersion frequencies (2, 4, 8, 16 or 20 h) and immersion times (5, 10 or 15 min) were tested on the TIS system. Repeated trials for immersion frequencies at short time intervals (10 min/2 h, 10 min/4 h etc.) showed hyper hydrated explants up to 100%, while this problem was not observed in immersions with relatively longer time intervals (10 min/20 h). The highest shoot forming capacity index (3.12) and the minimum hyper hydration rate (8%) were obtained in shoot tips with 10 min immersion frequency every 16 h. The protocol presented here, presents the initial stage of mass propagation of P. khinjuk Stocks cultures.en_US
dc.identifier.citationTilkat, E., Süzerer, V., Ersali, Y., Hoşer, A., Kılınç, F. M., Tilkat, E. A. ve diğerleri. (2014). Mass shoot proliferation of pistacia khinjuk stocks using temporary immersion bioreactor system (TIS). Acta Horticulturae, 1028, 145-152.
dc.identifier.doi10.17660/actahortic.2014.1028.24
dc.identifier.endpage152en_US
dc.identifier.isbn9789462610132
dc.identifier.issn0567-7572
dc.identifier.scopus2-s2.0-84899521068en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage145en_US
dc.identifier.urihttps://doi.org/10.17660/actahortic.2014.1028.24
dc.identifier.urihttps://hdl.handle.net/11468/23680
dc.identifier.volume1028en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherInternational Society for Horticultural Scienceen_US
dc.relation.ispartofActa Horticulturaeen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLiquid cultureen_US
dc.subjectMicropropagationen_US
dc.subjectPistachioen_US
dc.subjectRootstocksen_US
dc.subjectSemi-solid mediumen_US
dc.titleMass shoot proliferation of pistacia khinjuk stocks using temporary immersion bioreactor system (TIS)en_US
dc.typeConference Objecten_US

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