Effect of different transport temperatures on in vitro maturation of oocytes collected from frozen-thawed sheep ovaries

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dc.contributor.authorOzdas, Ozen Banu
dc.contributor.authorBaran, Alper
dc.contributor.authorTas, Muzaffer
dc.contributor.authorCirit, Umut
dc.contributor.authorDemir, Kamber
dc.contributor.authorBacinoglu, Suleyman
dc.contributor.authorPabuccuoglu, Serhat
dc.date.accessioned2024-04-24T17:21:11Z
dc.date.available2024-04-24T17:21:11Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of this study was to determine the effects of 2 different transport temperatures on the in vitro maturation of oocytes collected from frozen-thawed sheep ovaries. Sheep ovaries were transferred into saline at temperatures of 4 degrees C and 32 degrees C. After the 2 experimental groups (A: fresh cortex, B: frozen-thawed cortex) were formed, each group was divided into 2 subgroups (group A1: 4 degrees C, group A2: 32 degrees C [control]; group B1: 4 degrees C, group B2: 32 degrees C). The cortexes were dissected into slices 1-3 mm thick and pieces of 0.5 cm(2). For groups B1 and B2, 1-2 cortex pieces were placed in cryogenic vials containing 1 mL of freezing medium modified with Earle's salts (TCM-199) and supplemented with 10% fetal calf serum (FCS) (FCS + 2.5 M ethylene glycol + 0.1 M sucrose). The vials were then cooled to 7 degrees C at 2 degrees C/min and held at 7 degrees C for 10 min for manual seeding. The temperature was then lowered by 0.3 degrees C/min to -35 degrees C and thereafter by -10 degrees C/min to -75 degrees C. Vials were plunged into -196 degrees C liquid nitrogen and stored. Cortexes were thawed at 37 degrees C. Collected oocytes were matured in their own groups in 700 mu L of TCM-199 (supplemented with luteinizing hormone, follicle-stimulating hormone, pyruvate, and FCS) for 23 h in a gas mixture of 5% CO2, 5% O-2, and 90% N-2 at 38.8 degrees C. After maturation, oocytes were fixed in acetic acid and ethyl alcohol (1: 3) for 48 h. Oocytes were stained with aceto-orcein and then examined. At the end of the study, maturation rates for reaching metaphase I (MI) were similar in all groups (group A1: 30.76%, group A2: 38.09%, group B1: 30.65%, and group B2: 33.33%). The rates at which metaphase II (MII) was reached were 18.58%, 34.69%, 7.25%, and 6.48%, respectively. The best development was seen in group A2 (P < 0.001). Sheep oocytes obtained from fresh and frozen-thawed cortexes reached the MII stage if transported at 4 degrees C.en_US
dc.description.sponsorshipIstanbul University Research Fund [UDP-14403]en_US
dc.description.sponsorshipThis study was supported by the Istanbul University Research Fund, UDP-14403.en_US
dc.identifier.doi10.3906/vet-1104-24
dc.identifier.endpage19en_US
dc.identifier.issn1300-0128
dc.identifier.issue1en_US
dc.identifier.startpage15en_US
dc.identifier.trdizinid140950
dc.identifier.urihttps://doi.org/10.3906/vet-1104-24
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/140950
dc.identifier.urihttps://hdl.handle.net/11468/19396
dc.identifier.volume37en_US
dc.identifier.wosWOS:000313947600004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSheepen_US
dc.subjectOocyteen_US
dc.subjectMaturationen_US
dc.subjectOvary Cortexen_US
dc.subjectTransport Temperatureen_US
dc.titleEffect of different transport temperatures on in vitro maturation of oocytes collected from frozen-thawed sheep ovariesen_US
dc.titleEffect of different transport temperatures on in vitro maturation of oocytes collected from frozen-thawed sheep ovaries
dc.typeArticleen_US

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