In vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm

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dc.contributor.authorAkdemir, Huelya
dc.contributor.authorSuzerer, Veysel
dc.contributor.authorTilkat, Engin
dc.contributor.authorYildirim, Hakan
dc.contributor.authorOnay, Ahmet
dc.contributor.authorCiftci, Yelda Ozden
dc.date.accessioned2024-04-24T16:10:37Z
dc.date.available2024-04-24T16:10:37Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractAs genetic erosion of pistachio (Pistacia vera L.) has been occurring in the Mediterranean, Central and West Asia and North Africa, experiments were conducted to conserve two cultivars ('Atli' and 'Siirt') of mature pistachio germplasm by assessing both medium-and long-term conservation techniques. In medium-term conservation, our results showed that it was feasible to conserve both cultivars in the form of either microshoots or encapsulated shoot apices up to 12 months at 4 degrees C in the dark. As regards long-term conservation, encapsulation-dehydration and droplet-vitrification techniques were assessed for cryopreservation of cold-hardened and osmoprotected shoot apices of mature 'Atli' cultivar. Among the methods tested, 13.6% of regrowth was achieved with incubation of explants in the droplets of vitrification solution for 150 min at 0 degrees C followed by direct immersion in liquid nitrogen (LN), rapidly thawed and then cultured on Murashige and Skoog's (MS) medium containing 1 mg L-1 BA and 0.5 mg L-1 GA(3). The developed droplet-vitrification technique appeared as a promising procedure for long-term preservation of shoot apices of mature pistachio germplasm. Moreover, assesment of genetic fidelity by Random Amplified Polymorphic DNA analysis (RAPD) revealed out high levels of genetic stability between donor plant and cryopreserved plants (similarity indexes between 0.959 and 0.973) after they were subcultured for at least 3 months. The detected low level of genetic instability could be due to the toxic effect of PVS2 and regeneration phase. The optimized conservation techniques, especially slow growth storage, could be applied to preserve other Pistacia species.en_US
dc.description.sponsorshipTUBITAK, the Scientific and Technological Research Council of Turkey [209T030]; Gebze Institute of Technology [2009-A10]en_US
dc.description.sponsorshipThis study was funded by a grant (209T030), from TUBITAK, the Scientific and Technological Research Council of Turkey. The study was also partially funded by a grant (2009-A10) from Gebze Institute of Technology.en_US
dc.identifier.doi10.1007/s13562-012-0109-2
dc.identifier.endpage51en_US
dc.identifier.issn0971-7811
dc.identifier.issn0974-1275
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84871916565
dc.identifier.scopusqualityQ2
dc.identifier.startpage43en_US
dc.identifier.urihttps://doi.org/10.1007/s13562-012-0109-2
dc.identifier.urihttps://hdl.handle.net/11468/14970
dc.identifier.volume22en_US
dc.identifier.wosWOS:000316296700006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringer Indiaen_US
dc.relation.ispartofJournal of Plant Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDroplet-Vitrificationen_US
dc.subjectEncapsulation-Dehydrationen_US
dc.subjectGenetic Fidelityen_US
dc.subjectMature Pistachioen_US
dc.subjectSlow Growth Storageen_US
dc.subjectSynthetic Seeden_US
dc.titleIn vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasmen_US
dc.titleIn vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm
dc.typeArticleen_US

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